This document outlines guidelines for the care and use of laboratory animals. It discusses establishing quarantine procedures for new animals, providing veterinary care and disease surveillance, maintaining high standards of hygiene, and using anesthesia, analgesia and humane euthanasia practices. It also addresses maintaining suitable housing facilities with proper temperature, humidity and ventilation control, keeping detailed records, establishing standard operating procedures, training personnel, and transporting animals in a way that minimizes stress and risk of infection or injury. The overarching goals are to promote the humane treatment of research animals and avoid unnecessary pain or distress.

1. NSTITUTEOF PHARMACEUTICAL EDUCATION AND RESEARCH BORGAON (MEGHE)
WARDHA - 442001

2. CONTENTS
INTRODUCTION
GOALS
VETERINARY CARE
QUARANTINE
SURVEILLANCE
PERSONAL HYGIENE
ANAESTHESIA & EUTHANASIA
MISCELLANEOUS

3. INTRODUCTION
COMMITTEE FOR THE PURPOSE OF CONTROL AND SUPERVISION OF EXPERIMENTS ON
ANIMALS (CPCSEA)
STATUTORY BODY FORMED BY THE ACT OF THE INDIAN PARLIAMENT UNDER THE PREVENTION
OF CRUELTY TO ANIMALS ACT, 1960
FORMED IN 1964
REVIVED IN 1998, UNDER THE COMMITTED CHAIRPERSONSHIP OF MENEKA GANDHI.

4. GOALS
TO PROMOTE THE HUMANE CARE OF ANIMALS USED IN RESEARCH.
TO AVOID UNNECESSARY PAIN BEFORE DURING AND AFTER EXPERIMENT.
TO PROVIDE SPECIFICATIONS THAT WILL ENHANCE ANIMALS WELL BEING AND QUALITY OF
RESEARCH.

5. VETERINARY CARE
ADEQUATE VETERINARY CARE MUST BE PROVIDED
AND IT IS THE RESPONSIBILITY OF A VETERINARIAN OR A PERSON WHO HAS TRAINING OR
EXPERIENCE IN LABORATORY ANIMAL SCIENCES AND MEDICINE.
OBSERVE REGULARLY FOR SIGN OF ILLNESS, INJURY, OR ABNORMAL BEHAVIOUR.

6. QUARANTINE
SEPARATION OF NEWLY RECEIVED ANIMALS FROM THOSE ALREADY IN THE FACILITY UNTIL THE
HEALTH AND POSSIBLY THE MICROBIAL STATUS OF NEWLY RECEIVED ANIMAL HAVE BEEN
DETERMINE.
A MINIMUM DURATION OF QUARANTINE FOR SMALL ANIMAL-1 WEEK AND FOR LARGER
ANIMAL-6 WEEK.

7. SURVEILLANCE, DIAGNOSIS, TREATMENT
AND CONTROL OF DISEASE
ALL ANIMAL SHOULD BE OBSERVED FOR SIGNS OF ILLNESS, INJURY, OR ABNORMAL
BEHAVIOUR BY ANIMAL HOUSE STAFF
ANIMALS THAT SHOW SIGNS OF A CONTAGIOUS DISEASE SHOULD BE ISOLATED FROM HEALTHY
ANIMALS IN THE COLONY.

8. PERSONAL HYGIENE
ANIMAL CARE STAFF MAINTAIN A HIGH STANDARD OF PERSONAL CLEANLINESS.
USE DISPOSABLE GEAR SUCH AS GLOVES, MASKS, HEAD COVERS, COATS, COVERALLS AND SHOE
COVERS.
PERSONNEL SHOULD NOT BE PERMITTED TO EAT, DRINK, SMOKE OR APPLY COSMETIC IN
ANIMAL ROOMS.

9. ANESTHESIA
IT MUST ALSO BE ENSURED THAT THE ANAESTHESIA IS GIVEN FOR THE FULL DURATION OF
EXPERIMENT AND AT NO STAGE THE ANIMAL IS CONSCIOUS TO PERCEIVE PAIN DURING THE
EXPERIMENT.
SEDATIVES, ANALGESICS AND ANAESTHETICS SHOULD BE USED TO CONTROL PAIN OR DISTRESS
UNDER EXPERIMENT.

10. EUTHANASIA
EUTHANASIA IS RESORTED TO EVENTS WHERE AN ANIMAL IS REQUIRED TO BE SACRIFICED ON
TERMINATION OF AN EXPERIMENT OR OTHERWISE FOR ETHICAL REASONS.
THE PROCEDURE SHOULD BE CARRIED OUT QUICKLY AND PAINLESSLY IN AN ATMOSPHERE FREE
FROM FEAR OR ANXIETY.

11. PHYSICAL FACILITIES
BUILDING MATERIALS - MOISTURE-PROOF, FIRE-RESISTANT, SEAMLESS MATERIALS ARE MOST
DESIRABLE PEST RESISTANCE.
CORRIDOR - WIDE ENOUGH TO FACILITATE THE MOVEMENT OF PERSONNEL AS WELL AS
EQUIPMENT AND SHOULD BE KEPT CLEAN.
UTILITIES - WATER LINES, DRAIN PIPES AND ELECTRICAL CONNECTION.
ANIMAL ROOM DOORS - RUST, VERMIN AND DUST PROOF AND PROVIDED WITH AN OBSERVATION
WINDOW.
FLOORS - SMOOTH, MOISTURE PROOF, NON-ABSORBENT, SKID-PROOF.

12. WALLS & CEILINGS - FREE OF CRACKS,
STORAGE AREAS - SEPARATE STORAGE AREAS SHOULD BE DESIGNED FOR FEED, BEDDING,
CAGES AND MATERIALS NOT IN USE.
EXPERIMENTAL AREA - SEPARATE AREA FROM THE PLACE WHERE ANIMALS ARE HOUSED.

13. ENVIRONMENT
TEMPERATURE AND HUMIDITY CONTROL :- AIR CONDITIONING IS EFFECTIVE IN REGULATING
ENVIRONMENTAL PARAMETERS.
TEMPERATURE RANGE- 18 TO 29°C (64.4 TO 84.2°F)
RELATIVE HUMIDITY RANGE- 30% TO 70%
VENTILATION :-THE ANIMAL FACILITY AND HUMAN OCCUPANCY AREAS SHOULD BE
VENTILATED SEPARATELY.

14. RECORD KEEPING
1) THE ANIMAL HOUSE SHOULD MAINTAIN FOLLOWING RECORDS :
2) ANIMAL HOUSE PLANS, WHICH INCLUDES TYPICAL FLOOR PLAN, ALL FIXTURES ETC.
3) ANIMAL HOUSE STAFF RECORD.
4) HEALTH RECORD OF STAFF & ANIMALS.
5) BREEDING, STOCK, PURCHASE AND SALES RECORDS.
6) RECORDS OF EXPERIMENTS CONDUCTED WITH THE NUMBER OF ANIMALS USED
7) DEATH RECORD.

15. STANDARD OPERATING PROCEDURE
SOP SHOULD CONTAIN FOLLOWING ITEMS :-
NAME OF AUTHOR
TITLE OF SOP
DATE OF PREPARATION
REFERENCE OF PREVIOUS SOP OBJECTIVES
DETAILED INFORMATION OF THE INSTRUMENTS USED IN RELATION WITH ANIMALS.

16. PERSONNEL AND TRAINING
THE STAFF MUST BE PROVIDED WITH ALL REQUIRED PROTECTIVE CLOTHING (MASKS, APRONS,
GLOVES AND GUMBOOTS AND OTHER FOOTWEAR) WHILE WORKING IN ANIMAL ROOMS.
REGULAR MEDICAL CHECK-UP IS ARRANGED FOR THE WORKERS.
PERSONS WORKING IN ANIMAL HOUSE DO NOT EAT, DRINK, SMOKE IN ANIMAL ROOM

17. TRANSPORT OF LAB ANIMALS
THE MODE OF TRANSPORT,
THE CONTAINERS,
THE ANIMAL DENSITY IN CAGES,
FOOD AND WATER DURING TRANSIT,
PROTECTION FROM INFECTION, INJURIES AND STRESS.

18. REFERENCES
S.K GUPTA, PHARMACOLOGICAL & TOXICOLOGICAL SCREENING METHODS
HTTPS://WWW.PCI.NIC.IN/PDF/15.PDF
KULKARNI S K, COMMON LABORATORY ANIMALS (1999) HANDBOOK OF EXPERIMENTAL
PHARMACOLOGY, VALLABH PRAKASHAN, DELHI 3:11-18.

19. THANK YOU.