Potentially environmentally harmful subsidies far exceed finance for biodiversity conservation, and progress toward reducing them has been limited. The objective is to develop guidance for identifying and assessing subsidies harmful to biodiversity by drawing on existing national assessments. These vary in scope, sector coverage, and methodology. A proposed framework includes scoping subsidies, assessing impacts on biodiversity, and prioritizing reforms. Next steps are to expand the comparative analysis of assessments and update guidance for national identification of harmful biodiversity subsidies.