Recommandé
Recommandé
Contenu connexe
Similaire à Institute for Plant Sciences Update 8.29.17
Similaire à Institute for Plant Sciences Update 8.29.17 (20)
Dernier
Dernier (20)
Institute for Plant Sciences Update 8.29.17
- 1. Plant Sciences Update Tuesday, August 29, 2017 11:00 a.m. – 12:00 p.m. PFEN 241 (Deans Auditorium)
- 2. Introduction Karen Plaut Interim Dean, College of Agriculture Mitch Tuinstra, Scientific Director, Institute for Plant Sciences
- 3. About • Component of Purdue Moves, a series of university initiatives announced by President Mitch Daniels in 2013 • More than $20M invested in plant sciences since fall 2013 • Brings together multidisciplinary research and education • Developing phenotyping technology to enhance research, teaching, extension • Renamed the Institute for Plant Sciences to align with the other Life Science Institutes 3
- 4. Pipeline • Expand basic plant biology • Automate phenotyping to link genes with observable traits • Integrate data streams using robust analytic platform • Move technology from research to commercialization 4
- 5. Center for Plant Biology Clint Chapple Director, Center for Plant Biology
- 6. Center for Plant Biology Update Expanding research and education in basic plant biology
- 7. What is CPB? • Established through Purdue Moves to expand research and education in basic plant biology • Interdepartmental community of 32 faculty who share an interest in basic plant biology • Enhance graduate student training in basic plant sciences • Support research of participating members through collaboration, mentoring, and discussion • Renamed from Center for Molecular Agriculture to CPB to reflect its mission within the Institute for Plant Sciences 7
- 8. New Hires • 10 new faculty members – seven on campus, two arriving this semester, and one arriving January 2018 • Six – Department of Botany and Plant Pathology • Three – Department of Biochemistry • One – joint appointment in Department of Entomology and Department of Forestry and Natural Resources 8
- 9. Membership • Finalized membership agreements and faculty applications • 32 faculty from seven departments and two colleges • 10 members hired under Institute for Plant Sciences 9
- 10. Progress • Biweekly faculty meetings to discuss next steps and future initiatives • CPB Symposium associated with the Midwest ASPB meeting • Web presence and graduate student recruitment material being developed • Reduced rates for CPB faculty – Bindley Bioscience Center • CPB Seminar Series co-hosted with departments – starting in September • CPB Seed Grant program – proposals due in September and funding announcements in October 10
- 11. Molecular Agriculture Summer Institutes and Educational Initiatives Amy Jones Plant Sciences Recruitment and Outreach Coordinator
- 12. 12 Undergraduate MASI Communication Mentorship 7 8 9 8 2014 2015 2016 2017 Participants Funding for 10 students Funding Breakdown 1/3 1/3 1/3 Office of Academic Programs Faculty Home Dept. Faculty Funds Other Programmatic Costs: OAP $4,800 total stipend ● $1,600 per entity Research and Communication 5communication lunches 2Poster Presentations 40research hrs. weekly
- 13. 13 Pre-College MASI 13 16 18 2015 2016 2017 Participants 4 6 8 2015 2016 2017 Mentors Future: 25 students 47 TOTAL Future: Sustain 8 Mentors Increase involvement 4Departments Representing College Decisions 22 College-age 18 Applied to Purdue 15 Purdue Students 8 (50%+) in Ag 4 (25%+) in Engineering 3 in other Colleges Discovery Mentorship Belonging
- 14. Other Initiatives and Looking to the Future Pre-College Funding Questions Amy Jones jones176@purdue.edu Teaching Lab SpacesLearning Community Student Participation Undergraduate
- 15. Controlled Environment Phenotyping Facility (CEPF) Julie Hickman Plant Sciences Project Manager Chris Hoagland CEPF Facility Manager
- 16. Controlled Environment Phenotyping Facility (CEPF) • End of September 2017 – building shell construction complete • Fall 2017 – equipment delivered • Approx. 7 different vendors • Week of August 21 – Conviron large growth chamber arrived; assembly over next few weeks • Late September – conveyors and camera imaging systems arrive from Netherlands • February 2018 – equipment installation and initial testing complete 16
- 17. CEPF features • Conviron Growth House • 256 plant capacity Growth House with precise environmental controls • Automated conveyors for plant growth and randomization • Argus Multi-feed Nutrient Injection • Fully automated precise weight-based water dosing and water to saturation options • Single nutrient limitation with gradient control • Imaging System for plants up to 4m high • RGB – high throughput morphology, geometry, biomass, plant size, and color • Top-view and multiple side-view cameras • Segmentation: chlorophyll fluorescence to detect plant tissues • Up to 150 plants per hour • Hyperspectral • Color, vegetative indices, physiological status, and pigmentation • Up to 70 plants per hour 17
- 18. CEPF equipment factory acceptance 18 • The equipment for the CEPF is custom made. In August, we inspected and verified the conveying and camera equipment was correct for the facility.
- 19. ISAL software demonstration at Netherlands Grower 19 • Equipment review included a sneak peak of the ISAL software being developed to image and move our plants. • This software will enable the different vendors to communicate opening doors, watering, and imaging plants. • The software will enable us to capture images and will work with other packages, including the ones were are developing here at Purdue for data graphs and analysis.
- 21. Potential Applications • Seedlings to mid sized plants • Genotype performance • Fertility and water responses • Herbicide damage • Soil contaminants • Soil microbes 21
- 22. TopView Imager 22 • Imaging cabinet for basic phenotyping • Captures eight spectral bands • Stitches Red, Green, and Blue bands to form a RGB image • Automatically segments data into parameters and histograms • Starting point for digital phenotyping
- 23. Time Lapse Image Data and Leaf Count 23 Channel Color Wavelength (nm) 0 Deep blue 450 1 green 520 2 yellow 590 3 amber 634 4 red 660 5 hyper red 730 6 infra red 870 7 white 400-700 Leaf count
- 24. Parameter Data plant nr length (mm) width (mm) center x center y grid row grid col plant area (mm2) plant convex hull area (mm2) longest radial (mm) roundness avg hue avg sat avg val plant 1 140.46 619.419 1442 224 0 0 218778 367050 387.243 232.997 65.485 8.11198 124.632 plant 2 213.285 619.419 1646 407 0 0 469879 614042 358.576 67.0578 58.9705 8.94594 115.557 plant 3 163.005 619.419 1544 277 0 0 297110 443456 371.716 166.096 57.8946 8.69574 121.652 plant 4 20.6042 55.4963 1358 1781 0 0 3451.65 4591.14 36.9934 36.3893 30.8044 69.3358 60.5648 plant 5 77.1741 91.8922 1701 1859 0 0 12079.4 34709.3 65.5914 256.655 29.874 63.5456 64.4642 plant 6 25.8489 46.5033 2052 1592 0 0 3935.48 5922.76 28.9074 53.5608 28.3247 71.6823 71.8321 24 • Automatically segments masked image • Plant characteristic data for each plant • Calculates a mean for each Hue Saturation and Value (HSV) • Histogram for HSV and gray scale • Provides repeatable objective measurements • User friendly interface Segmented data from Topview imager
- 25. Indiana Corn and Soybean Innovation Center Jason Adams Advance Phenotyping & Plant Processing Facility Manager
- 26. ICSC Users • Approx. 40 different labs or groups have used the facility • During the winter and spring the seed processing areas became very active • Threshing and shelling • Plant and seed grinding • Seed treating • Seed counting and weighing • Root washing and scanning • Summer has brought in higher numbers to the imaging and sensor development areas • Pilot work area • PhenoRover development • Break room for ICSC and ACRE users • Meeting space for labs or groups 26
- 27. ICSC Tours 27 180 individual tours with a total of over 3300 guests • Over 40 groups were student groups, including Bourlaug Scholars, MASI, FFA, 4-H, Mandela Washington Fellows and Ivy Tech • Individuals or groups from over 20 countries have walked through ICSC
- 28. New and Donated Equipment 28 • Ear Photometry Box with the capability to make approximately 30 different ear and kernel measurements • 2 Root washing stations for washing root samples down prior to measurements • 1 large ice machine for research use • 2 Li-COR 6400 Portable Photosynthesis System
- 29. Meeting and Classroom Space 29 • Student Innovation Hub now has projector and large screen • Three small conference rooms equipped with phones; one also equipped with a monitor • Data command converted into dual purpose room; now equipped with projector, large screen, and 22 classroom seats
- 30. Indiana Corn and Soybean Endowment Innovation Grants - 2017 • Development of a Plant Tattoo for Managing Crop Stress – d development of chromatic biosensors for heat stress and ABA content • Chongli Yuan, Chemical Engineering, Alexander Wei, Chemistry • Development of a Universal Method for High-Throughput Automated Root Phenotyping to Identify Root Traits in Soybean • Gordon McNickle and Anjali Iyer-Pascuzzi - Botany and Plant Pathology, Jian Jin- Ag and Biological Engineering 30
- 31. 2017 Precision Measurement Zone & Contracted Flights Rick Westerman Data Analyst Bioinformatics Specialist
- 32. First full year • Flights from May 2017 through the present • Our own 5-band multispectral camera (RedEdge) • Our own flying platforms 32 3DR Solo
- 33. Contracted flights • ~100 acres • All maize • Bi-weekly flights • Low cost 33 RGB NDVI (false color) Close up (brown areas are Bare spots between plots)
- 34. PMZ (Precision Measurement Zone) • ~25 acres • Wheat, sorghum, soybean, maize • Weekly flights • Some ground measurements included • No cost 34 Wheat (late season) Sorghum & Maize Soybean
- 35. Data delivery • Atlas for first pass • Rectified to GCP (ground control points) • Plot-level NDVI extraction if feasible 35
- 36. Plot NDVI extraction 36 Plot Treatment 2017-06-25 2017-07-09 2017-07-28 303 45 0.40 0.67 0.72 304 23 0.38 0.70 0.75 2017-06-25 2017-07-09 2017-07-28 (NDVI – near infared versus red)
- 37. Experiences and further work • Remainder of 2017 More flights More plot-level data Ground truthing – own, researcher, ARPA-E! Further development of data delivery methods • 2018 Same camera and flight platforms Anticipate more serving more researchers 37
- 38. Ag-Celerator Tim Peoples Managing Director, Purdue Foundry
- 39. Foster Entrepreneurship • Ag-celerator Summary • 28 involved in Launchbox excluding students • 2 rounds, 4 companies funded – Phicrobe, HyroGrow, Akanocure, Vinsense Phicrobe -designed to enhance food safety in plant based products
- 40. Round 2 – Ag-Celerator winners Akanocure - Bioinspired molecule development for agrochemicals Vinsense – decision making tool for water use in crops
- 41. Ag-Celerator Fall 2017 Timeline Sept 14 Sept 28 Oct 5 Oct 12 Oct 16- Nov 22 Nov 26 Nov 27 Dec 18 Dec 19 Questions to pvinfo@prf.org Callout Application Deadline Semi-finalists Announced Mandatory Semi-finalist Meeting Firestarter Training at the Foundry Final Materials Upload Deadline Finalists Announced Demo Day Awards Announced
- 42. ASPIRE Internship Nathan Mosier Professor, Agricultural & Biological Engineering Chair, ISA Soybean New Uses
- 43. 26 Years of ISA Student Soybean Product Innovation Competition • 16 Teams completed the 2016-2017 competition, 5 provisional patents • 50 students • Representing 20 different majors from the Colleges of Agriculture, Engineering, Pharmacy, Science, and Business (5) • Focuses on generating product ideas and proofs of concept with preliminary market analysis
- 44. Execution • Pilot program Summer of 2017 • Students who completed the contest invited to apply • 6 Student interns selected to work on 5 projects • Projects = product ideas from competition (provisional patents, other vetted concepts) • Partnership between Purdue Foundry and Laboratory of Renewable Resources Engineering/ABE for student mentoring and oversight Funding • Purdue Ag-celerator Program ASPIRE Ag Soy Product Innovation Realization and Entrepreneurship Internship Program
- 45. ASPIRE Program • FIRESTARTER: “Bootcamp” • Customer DiscoveryEntrepreneurship • Business Plan Development • Networking and Entrepreneur-in-Residence Mentoring Micky Creech Competition and ASPIRE Coordinator 2017 ASPIRE Interns
- 46. ASPIRE Ag-Celerator Demo Day • 6 Teams pitched their companies to the Ag-Celerator Selection Committee on August 18, 2017. • Recommendations for funding were made (official announcements forthcoming) NeoSeed SoyaMein Absoybeancy Soy Metal Adsorbent SoyPoofession FiltraSoy
- 47. Questions? 47