Thin layer chromatography can be used to monitor the progress of a reaction, identify compounds present in a given mixture, and determine the purity of a substance. Specific examples of these applications include: analyzing ceramides and fatty acids, detection of pesticides or insecticides in food and water, analyzing the dye composition of fibers in forensics, assaying the radiochemical purity of radiopharmaceuticals, or identification of medicinal plants and their constituents

1. Thin Layer Chromotography
By Dr. Robert D. Craig, Ph.D
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2. Thin layer safety
• Please do not walk around with sample jar

3. Please do not let the solvent front
sit too long!!

4. You will have the wrong Rf value

5. Please only tap the capillary on the
plate

6. Please use the correct side of the plate

7. The instructors will do the UV analysis

8. Good luck!!!