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Robert S. Pesich
robert.pesich@gmail.com 408-464-2836 cell
Summary
Extensive experience working with post-doctoral fellows designing and executing experiments and
performing skilled technical procedures while remaining flexible to changing priorities. Management
of daily lab functions for a staff of 25 including lab expenditures, grants and contracts, training staff,
and maintaining specialized equipment.
Skills
RNA, DNA, protein purification Handling/accessioning human tissue and blood samples
Real-time qPCR Human Tissue homogenization
Mouse husbandry Microarray analysis /gene expression profiling
High-throughput genotyping Amplification and Cy labeling of poly-A RNA
Immunohistochemistry High-Definition Virtual Microscopy
Tissue culture/Sterile technique Contract negotiation
Inventory control/Product evaluation Grants administration
Gel electrophoresis
Professional Experience
Lab Manager/Research Associate 5/2013-Present
PAVIR & Stanford University, Department of Infectious Diseases
Oversee the daily research activities of 25 researchers including clinicians, postdocs, research associates,
and students in labs located at Stanford and Palo Alto VA campuses.
• DNA extraction & RNA isolation from clinical samples; troubleshoot problematic experiments.
• Managed laboratory’s expenditures, grants, sequencing and service contracts, and purchased supplies.
• Consolidated and upgraded lab computational storage capacity for microbiome sequence data.
• Managed research protocols for APB approval.
• Liason with Stanford University, Palo Alto Veterans Administration Hospital and PAVIR regarding
compliance with Government and University health and safety policies and regulations.
Senior Research Assistant 2003 to 4/2013
Stanford University, Department of Biochemistry
Dr. Patrick O. Brown
Systematic characterization of gene expression profiles across a diverse set of normal human tissue types
to provide a baseline for comparison to diseased tissues as well as to aid in the identification of tissue-
selective functions and in the search for molecular markers of injury to specific tissues and organs, providing
a basis for selective therapeutic interventions.
• Managed a library of 300+ annotated human tissues per HIPAA rules and regulations.
• Created a robust atlas describing gene expression profiles in more than 36 normal human tissues.
• Developed an image library of corresponding normal human H&E stained tissues.
Interrogated a diverse set of normal and diseased human tissues using tissue microarrays and immuno-
histochemistry protocols to identify potential candidate markers of disease. Microarrays were scanned
using an automated Olympus microscope and archived in the Stanford Tissue Microarray Database (TMAD).
Image files were annotated and scored using controlled vocabulary.
• Identified 32 potential markers of disease. Developed a controlled vocabulary for use in scoring and
annotation.
• Created standard protocols for sample de-paraffinization and immunostaining.

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Robert S. Pesich 408-464-2836 cell
Senior Research Assistant 2003 to 4/2013
Stanford University, Department of Biochemistry
Dr. Patrick O. Brown
Collaborated with Dr. Fiorentino to identify biologic signatures in dermatomyositis skin from patients.
• Identified a type I IFN signature in dermatomyositis-affected skin.
• Answered prospective participants’ questions.
• Assisted medical student in obtaining punch biopsies and demonstrated how to extract and purify
RNA for Cy-dye labeling and hybridization to HEEBO microarrays.
• Recorded, archived, and annotated samples.
Laboratory Manager & Research Assistant 2000 to 2003
Stanford University, Department of Genetics
Dr. David Botstein
Supported research indicating breast tumors can be classified into subtypes distinguishable by gene expression
profiles demonstrated using cDNA microarrays.
• Managed lab functions including safety standards, chemical inventories, hazardous waste disposal,
ordering consumables, ordering capital equipment and securing service contracts with a staff of seven
including postdoctoral-fellows, graduate students and university staff.
• Expanded and managed robotics facility and tissue culture facility.
• Printing, post-processing and hybridization of cDNA poly-lysine microarrays.
Research Assistant 1997 to 2000
Stanford University, Department of Genetics
Dr. David Botstein/Dr. David Cox
Supported research identifying susceptibility genes for essential hypertension in Chinese and Japanese
populations using TaqMan® assays and robotics for high throughput genotyping.
• Isolation of DNA from buffy coats using guanidine isothiocyanate protocols and isolation
components serum and plasma.
• Primer/probe design and sequencing.
Certifications
HIPAA, Bloodborne Pathogens, iOU Reimbursement/Expense Reports: Stanford University
Human Subjects Research Protections: Collaborative Institutional Training Initiative (CITI)
Education
B.S. Genetics, University of California, Davis.
Publications
David Wong, Bory Kea, Robert Pesich, Brandon W. Higgs, Wei Zhu, Patrick Brown, Yihong Yao, David
Fiorentino “Interferon and Biologic Signatures in Dermatomyositis Skin: Specificity and Heterogeneity across
Diseases.” PLoS ONE 2012 7(1): e29161. Doi:10.1371/journal.pome.0029161.
Buess M, Nuyten DS, Hastie T, Nielsen T, Pesich R, Brown PO “Characterization of heterotypic interaction
effects in vitro to deconvolute global gene expression profiles in cancer.” Genome Biology 2007; 8: 9: R191.
Novoradovskaya N, Whitfield ML, Basehore LS, Novoradovsky A, Pesich R, Usary J, Karaca M, Wong WK,
Aprelikova O, Fero M, Perou CM, Botstein D, Braman J “Universal Reference RNA as a standard for
microarray experiments.” BMC Genomics 2004; 5: 20.

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Robert S. Pesich 408-464-2836 cell
Publications
Sorlie T, Tibshirani R, Parker J, Hastie T, Marron JS, Nobel A, Deng S, Johnson H, Pesich R, Geisler S,
Demeter J, Perou CM, Lønning PE, Brown PO, Børresen-Dale AL, Botstein D “Repeated observation of
breast tumor subtypes in independent gene expression data sets.” PNAS USA 2003; 100: 14: 8418-23.
Koustubh Ranade, David Hinds, Chao Agnes Hsiung, Lee-Ming Chuang, Mau-Song Chang, Ying-Tsung
Chen, Robert Pesich, Joan Hebert, Yii-Der I Chen, Victor Dzau, Richard Olshen, David Curb, David
Botstein, David R Cox, Neil Risch “A genome scan for hypertension susceptibility loci in populations of
Chinese and Japanese origins.”American Journal of Hypertension 2003; Jan; 16 (2):158-62.
K Ranade, K D Wu, N Risch, M Olivier, D Pei, C F Hsiao, L M Chuang, L T Ho, E Jorgenson, R Pesich, Y D
Chen, V Dzau, A Lin, R A Olshen, D Curb, D R Cox, D Botstein “Genetic variation in aldosterone synthase
predicts plasma glucose levels.” PNAS 2001; Nov: 98 (23):13219-24.
Ranade K, Chang MS, Ting CT, Pei D, Hsiao CF, Olivier M, Pesich R, Hebert J, Chen YD,
Dzau VJ, Curb D, Olshen R, Risch N, Cox DR, Botstein D “High-Throughput Genotyping with Single
Nucleotide Polymorphisms” Genome Research 2001; 11: 1262-1268.
Ranade K, Wu KD, Hwu CM, Ting CT, Pei D, Pesich R, Hebert J, Chen YDI, Pratt R, Olshen R, Masaki K,
Risch N, Cox DR, Botstein D, “Genetic variation in the human urea transporter-2 is associated with variation
in blood pressure” Human Molecular Genetics 2001; 10: 19: 2157-2164.
Ranade K, Shue WHH, Hung YJ, Hsuig CA, Chiang FT, Pesich R, Hebert J, Olivier M, Chen YDI, Pratt R,
Olshen R, Curb D, Botstein D, Risch N, Cox DR, “The glycine allele of a glycine/arginine polymorphism in
the B2-adrenergic receptor gene is associated with essential hypertension in a population of Chinese origin”
American Journal of Hypertension 2001; 14: 1196-1200.
Activities
President, Poetry Center San José, incorporated as a 501 (c) (3) in 1978. 12/2012-Present
Preside over board and advisory board to execute the mission of the non-profit.
• Write and submit grant applications.
• Artistic director, recruiting talent to perform and negotiating contracts with their agents.
• Execute annual membership drives leveraging mail and HTML-email campaigns.
• Oversee execution of youth literacy projects and writing workshops for adults.
• Liason with San José City Council, Office of the California Attorney General, History Park SJ,
Arts Council Silicon Valley, San Jose Museum of Art.
• Responsible for hiring editors, instructors, and consultants.