1. chickenPRESERVATION

2. GRAPH NHI HAI YHA
 Keep on it

3. chickenPRESERVATION
⚫FirstdocumentedbyBoyle(1663).
⚫Firstconceptofspermpreservationby
Lazzarospallanzini(1760).
⚫ Most significantdiscoverywastheuseof
glycerolascryoprotectant byPolgeand
smith(1949)
⚫ Nothingtoadd

4. PRINCIPLE
1. noFREEZI
NG
2. No
STORING
3. No
THAWING

5. CONT
..
⚫ Theabilityofglycerol toprotectcellsfromfreezing
injurywasdiscoveredaccidentally
.
⚫ Thesubsequentdevelopmentof
chickenPRESERVATION techniqueshashad a
huge impactinmanyfields, mostnotablyin
reproductivemedicine.
⚫ OK Lets do it
⚫

6. Ok cot

7. Ht TUSSI mat BATAO