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BY
OGUOYIBO .J. SISAN
Outline
 Background
 Bioremediation
 Enzymes
 Major enzymes used in bioremediation
 Case Study
 Advantages of biorem...
Background
A large number of enzymes
from bacteria, fungi, and
plants are involved in the
biodegradation of toxic
organic...
Bioremediation
Enzymes
Enzymes are biological catalysts that facilitate the
conversion of substrates into products by providing
favourab...
Fig 2:Mechanism of enzyme action
Enzymes Used In Bioremediation
(Contd)
Microbialperoxidases
Microbial
oxidoreductases
 Microbial Laccases
Microbial Ox...
Case Study of Enzymes Used in
Bioremediation
Very effective in the bioremediation of PAHs
 LIPs catalyse reactions in th...
Horseradish Peroxidase
 HRP is a proxidase that is secreted by the root hairs
of the horseradish plant
 Catalyse oxidati...
Manganese Peroxidase
MnP is a
hydrogen peroxide
dependent enzyme,
but it can only
oxidize organics
when in the
presence o...
Manganese Peroxidase(Contd.)
MnP oxidizes Mn(II) to Mn(III), which acts as an
obligatory oxidation intermediate for the o...
Advantages of Bioremediation
It is relatively expensive
No building needs to be dug up and removed
The method has been ...
Limitations of Bioremediation
Bacteria direction difficult to control
Lots of germs will be spread around
Most people a...
Conclusion
Bioremediation is a powerful tool to clean up
contaminated sites.
Regardless of which aspect is used, it offe...
References
R. S. Peixoto, A. B. Vermelho, and A. S. Rosado; 2011;
Petroleum-Degrading Enzymes: Bioremediation and New
Pro...
Enzymes and their roles in the bioremediation
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Enzymes and their roles in the bioremediation

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Enzymes and their roles in the bioremediation

  1. 1. BY OGUOYIBO .J. SISAN
  2. 2. Outline  Background  Bioremediation  Enzymes  Major enzymes used in bioremediation  Case Study  Advantages of bioremediation  Limitations of bioremediation  Conclusion  References
  3. 3. Background A large number of enzymes from bacteria, fungi, and plants are involved in the biodegradation of toxic organic pollutants. Bioremediation is the use of living organism for the recovery or clean up of contaminated medium. Environment Atmosphere Earth Water Space Fig.1: contaminated medium
  4. 4. Bioremediation
  5. 5. Enzymes Enzymes are biological catalysts that facilitate the conversion of substrates into products by providing favourable conditions that lower the activation energy of the reaction. They have active sites. They function based on their classification according to the E.C Number system.
  6. 6. Fig 2:Mechanism of enzyme action
  7. 7. Enzymes Used In Bioremediation (Contd) Microbialperoxidases Microbial oxidoreductases  Microbial Laccases Microbial Oxygenases Monooxygenases Microbial dioxygenases
  8. 8. Case Study of Enzymes Used in Bioremediation Very effective in the bioremediation of PAHs  LIPs catalyse reactions in the presence of hydrogen peroxide Lignin peroxidase obtained from Phanerochaete chrysosporium is effective against methylene blue and azure B dyes Lignin Peroxidase
  9. 9. Horseradish Peroxidase  HRP is a proxidase that is secreted by the root hairs of the horseradish plant  Catalyse oxidation of phenols, biphenols, anilines and benzidines etc.
  10. 10. Manganese Peroxidase MnP is a hydrogen peroxide dependent enzyme, but it can only oxidize organics when in the presence of Mn(Il). . hanism of MnP Figure 3: Mechanism of Manganese Peroxidase
  11. 11. Manganese Peroxidase(Contd.) MnP oxidizes Mn(II) to Mn(III), which acts as an obligatory oxidation intermediate for the oxidation of of various compounds. The Mn(IlI) ions migrate away from the enzyme and start the oxidation of the lignin and other compounds. compounds.
  12. 12. Advantages of Bioremediation It is relatively expensive No building needs to be dug up and removed The method has been used effectively on oil spills and clean ups in the past As long as there is toxic waste, the bacteria continues to feed on it.
  13. 13. Limitations of Bioremediation Bacteria direction difficult to control Lots of germs will be spread around Most people are concerned that these bacteria may be able to make people sick.  It is limited to those compounds that are biodegradable
  14. 14. Conclusion Bioremediation is a powerful tool to clean up contaminated sites. Regardless of which aspect is used, it offers a safer and more cost effective way to clean up contaminated sites. It’s advantages generally outnumber its disadvantages owing to the number of sites that have been cleaned up using this method and its increasing popularity.
  15. 15. References R. S. Peixoto, A. B. Vermelho, and A. S. Rosado; 2011; Petroleum-Degrading Enzymes: Bioremediation and New Prospects; Volume 2011; 1-7. Prezi.com Snehal S. Menhon slide share https://www.khanacademy.org https://www.scribd.com http://www.slideshare.net/vanithagopal/bioremediation- 41934065 http://www.kolumbus.fi/ilona.barlund/martinsprojects.htm l

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