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Bioprinting and bionks a new paradigm for 3 d organ development

This presentation covers a brief introduction to 3D bioprinting and bioinks. Also give a glimpse of techniques, technology, types of bioprinting.

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Bioprinting and bionks a new paradigm for 3 d organ development

  1. 1. Presented by – Sushant B. Jadhav (18PBT206) Under the guidance of – Prof. Vandana B. Patravale 1
  2. 2.  The aim of 3D bioprinting is to construct, in vitro, tissues, organs and other biological systems that mimic their native counterparts  The materials used consist of natural and synthetic polymers, living cells, drugs, growth factors  3D bioprinting is an interdisciplinary field, requiring knowledge from developmental biology, stem cell science, chemistry, computer science, and materials science 2
  3. 3. Deciding application Selection of cells Selection of biomaterial Formulating bioink Deciding technique of bioprinting Fabrication process Computer program Bioprinting Validation and application 3
  4. 4. 2. Bioprinting technology Droplet- based Inkjet bioprinting Acoustic- droplet- ejection bioprinting Micro- valve bioprinting Extrusion- based Laser-induced forward transfer Stereolitho- graphy 4
  5. 5. Design Factors and the capabilities of Bioprinting Design Considerations in Bioprinting Bioprinting Fabrication Procedures Methods to enhance Bioprinting 5
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  7. 7. 7  Shape and Resolution  Material Heterogeneity 1. Heterogeneous Cells 2. Heterogeneous Biomaterials 3. Graduated Growth Factors  Cellular-Material Remolding Dynamism
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  9. 9. 9 Bioprintin g Fabricatio n Procedure s Direct Printin g In- Process Crosslin king Hybrid Printin g Indirec t Printin g Post- Process Crosslin king
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  11. 11.  Integrating Native and Engineered Tissue  Coordinating Cell-Material Interaction  Smart Biomaterials 1. Self-Assembly Materials 2. Stimulus Responsive Hydrogel  Power Simulation 11
  12. 12. 12 4. Bio- inks Hydrogels Photocrosslin kable inks Thermo- responsive inks Microcarriers Cell aggregates Cell pellets Tissue spheroids Tissue strands Decellularize d matrix components
  13. 13. 13 Natural and naturally- derived polymers Synthetic polymers Gelatin Pluronics Collagen poly(N- isopropylacrylamide) (PNIPAAM) Elastin Fibroin Fibrin Alginate Hyaluronic acid (HA)
  14. 14.  Functionalization for improving mechanical integrity  Functionalization for improving printability  Functionalization for enhancing biocompatibility and bioactivity 14
  15. 15. 15 Figure :Schematic summary of various methods of functionalization
  16. 16. Tissue engineering and regenerative medicine Transplantation and clinical applications Drug testing and high-throughput screening Cancer research 16
  17. 17. 7.1 Pluronic F127 hydrogel characterization and biofabrication in cellularized constructs for tissue engineering applications  Emilia Gioffredi et. al. developed a method for printing cellularised scaffolds from thermosensitive hydrogels. 17 Figure : A, B) FEG-SEM micrographs of a four-layered hydrogel scaffold (needle Ø 200 μm, fiber spacing 600 μm); C, D) transversal sections of the fabricated fibers.
  18. 18. 7.2 Tailoring mechanical properties of decellularized extracellular matrix bioink by vitamin B2-induced photo- crosslinking  Jinah Jang et. al. developed two-step process to solidified bio- ink. 18 Figure : Schematic illustration of a two-step crosslinking mechanism that applies concurrent crosslinking of vitamin-B2-induced covalent crosslinking and thermal crosslinking
  19. 19.  Bioprinting is one of the promising technology expanding its horizon  Integration of bioprinting techniques to enhance the printing process is a good strategy  Bio-inks are core of bio-printing  Different bio-inks with different bio-printing qualities thus we need to choose one according to our need  There is no idle bio-ink for printing thus each bio-ink can be functionalized for increasing its property according to need of technique  Synchronization of bioprinting and bioinks can definitely aid researchers to develop new applications of it 19
  20. 20.  Gudapati H, Dey M, Ozbolat I., 2016, “A comprehensive review on droplet-based bioprinting: Past, present and future”, Volume 102, Pages 20-42.  Ji Eun Kim, Soo Hyun Kim, Youngmee Jung, 2016, “Current status of three-dimensional printing inks for soft tissue regeneration”, Volume 13, Pages 636-646.  Jia Min Lee and Wai Yee Yeong, 2016, “Design and Printing Strategies in 3D Bioprinting of Cell-Hydrogels: A Review”, Adv. Healthcare Mater., Volume 5, Pages 2556-2565.  Ilze Donderwinkel, Jan C. M. van Hest and Neil R. Cameron, 2017, “Bio-inks for 3D bioprinting: recent advances and future prospects”, Polym. Chem., Volume 8, Pages 4451-4471.  Ozbolat IT, Yu Y., 2013, “Bioprinting toward organ fabrication: challenges and future trends”, IEEE Trans Biomed Eng, Volume 60, Pages 691-699. 20
  21. 21. 21 THANK YOU