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  2. WHY IT IS DONE The urine is analysed for:  Daily excretion of substances e.g urea, creatinine and vitamin C  Poisons e.g heavy metals  Drugs e.g morphine, sulphonamides  Mycobacterium tuberculosis  Sex hormones , adrenal and pituitary glands
  3. CONT….  Estimation of excretion of radioactive hormones.  In case of total parenteral nutrition (TPN) all the urine collected during 24 hrs is sent to the laboratory twice a week to obtain the excretion rate of urea and electrolytes
  4. PREPARATION  To prevent contamination of the urine, the bedpan or urinal must be scrupulously clean.  A woman patient is asked to wash her vulva before passing the urine, and not to contaminate the urine with faeces or toilet paper.  A large sterile bottle and funnel are covered with a sterile towel and kept in a safe place for receiving the urine.
  5. PROCEDURE  The patient is asked to empty his or her bladder at 8 a.m. on two successive days. The first specimen is discarded but all the urine passed after that, including the second 8 a.m. specimen, is poured into the large sterile means of the funnel.
  6. CONT….  If the urine is being collected for the assay of hormones, 10 ml of concentrated HCl is poured into the bottle, and during the period of collection the bottle is kept in a bucket of ice water and chips.