Necrosis is “Irreversible death of cells or tissues within a living organism, typically as a result of injury, infection, or inadequate blood supply”. It is a pathological process characterized by the breakdown of cellular components and the release of cellular contents, which can lead to inflammation and further tissue damage. Type of cell death, that is associated with loss of membrane integrity and leakage of cellular contents culminating in dissolution of cells, largely resulting from the degradative action of enzymes on lethally injured cells. Leaked cellular contents often promote a local host reaction, called inflammation. Enzymes responsible for digestion of cell, may be derived from lysosomes of the dying cells themselves and from lysosomes of leukocytes (that are recruited as a part of inflammatory reaction to dead cells. Leakage of intracellular proteins through the damaged cell membrane and ultimately into the circulation provides a means of detecting tissue-specific necrosis using blood or serum samples. E.g. Cardiac muscle, contains a unique isoform of the enzyme creatine kinase and of the contractile protein troponin. Hepatic bile duct epithelium contains the enzyme alkaline phosphatase. Hepatocytes contain transaminases. Irreversible injury and cell death in these tissues elevate the serum levels of these proteins, which makes them clinically useful markers of tissue damage.