Undergraduate Thesis Defense Slides
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This document summarizes an experiment testing the effects of single gene mutations on density-dependent sex differentiation in the fern Ceratopteris richardii. 11 mutant strains and a wild type strain were grown under controlled conditions and the number and sex of gametophytes were counted. Most mutant strains showed similar trends of increasing male percentage with density as the wild type. However, two strains, stl2 and rum1, showed significant differences in male percentage but normal growth, suggesting an altered response to the sex pheromone for those strains. The results provide insight into how genetic mutations can influence sex determination mediated by population density cues.
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Undergraduate Thesis Defense Slides
- 1. Density-Dependent Sexual Differentiation in Single-Nuclear Mutants of Ceratopteris richardii Heather E. Jordan Biology Department College of Health and Natural Sciences Lake Superior State University Sault Ste. Marie, MI 49783
- 9. Summary of Strains Investigated GenotypeCode Generic Name RNWT 1 Wild Type abr48 Abscisic Acid Tolerant cp Polka Dot det30 Non-Etiolated fdr1 FUDR Tolerant her1 Maleless him1 Highly Male rum1 Rumpled slo1 Slo-Mo Sperm stl2 Salt Tolerant zzz1 Sleepy Sperm
- 10. 06/03/09 Methods & Materials
- 12. 06/03/09 Experimental setup of the culture dome for each genotype:
- 13. Temperature: 28 o C pH = 6 Photoperiod : 24 hour
- 17. 06/03/09
- 19. 06/03/09 Results
- 20. 06/03/09 Could there be a trend? 14.5 89.9 cp 17.6 135.9 zzz1 13.5 136.3 det30 98.8 140.8 him1 13.9 157.1 slo1 0.00 211.9 her1 15.4 249.3 stl2 36.5 249.3 abr48 28.9 255.2 fdr1 19.8 281. 2 rum1 34.6 360.9 RNWT1 Mean % Male Mean # of Gametophytes Strain
- 21. 06/03/09 F Values 22.12 26.90 4.01 RNWT1 vs. cp 13.26 15.77 4.01 RNWT1 vs. zzz1 21.10 16.66 4.01 RNWT1 vs. det30 295.66 16.38 4.01 RNWT1 vs. him1 24.69 13.54 4.01 RNWT1 vs. slo1 87.16 6.06 4.01 RNWT1 vs. her1 18.30 2.92 4.01 RNWT1 vs. stl2 0.16 3.18 4.01 RNWT1 vs. abr48 1.51 2.80 4.01 RNWT1 vs. fdr1 11.39 1.66 4.01 RNWT1 vs. rum1 Mean % Male Mean # Gametophytes Fcrit
- 22. 06/03/09 The percentage of males increases with a rise in the population density across each strain.
- 23. 06/03/09 The overall composition of each genotype sample: