2nd picture is COVID gel picture. It's sideways and should be right side up given is a picture of gel electrophoresis in wich different DNA samples are loaded in different wells of gel. The DNA fragments migrated according to there size with the largest remaking closest to the well. Module II: Staining Agarose Gels Using FlashBlue TM 2. 5. 1. DILUTE 10mL of 10x concentrated FlashBlue 1N with 90mL of water in a flask and MIX well. 2. REMOVE the agarose gel and casting tray from the electrophoresis chamber. SLIDE the gel off of the casting tray into a small, clean gel- staining tray. 3. COVER the gel with the 1x FlashBlue M stain solution. STAIN the gel for 5 minutes. For best results, use an orbital shaker to gently agitate the gel while staining. STAINING THE GEL FOR LONGER THAN 5 MINUTES WILL REQUIRE EXTRA DESTAINING TIME. 4. TRANSFER the gel to a second small tray. COVER the gel with water. DESTAIN for at least 20 minutes with gentle shaking (longer periods will yield better results). Frequent changes of the water will accelerate destaining. 5. Carefully REMOVE the gel from the destaining liquid. VISUALIZE results using a white light visualization system. DNA will appear as dark blue bands on a light blue background. 6. ESTIMATE the base pair length of each fragment by comparing the distance each fragment traveled from the well to the bottom of the gel to the distance that each Standard DNA Marker fragment migrated. For a more accurate size calculation you can use a standard curve (see Appendix C). 7. Fill out the table below..

1. 2nd picture is COVID gel picture. It's sideways and should be right side up
given is a picture of gel electrophoresis in wich different DNA samples are loaded in different wells
of gel. The DNA fragments migrated according to there size with the largest remaking closest to
the well.
Module II: Staining Agarose Gels Using FlashBlue TM 2. 5. 1. DILUTE 10mL of 10x concentrated
FlashBlue 1N with 90mL of water in a flask and MIX well. 2. REMOVE the agarose gel and casting
tray from the electrophoresis chamber. SLIDE the gel off of the casting tray into a small, clean gel-
staining tray. 3. COVER the gel with the 1x FlashBlue M stain solution. STAIN the gel for 5
minutes. For best results, use an orbital shaker to gently agitate the gel while staining. STAINING
THE GEL FOR LONGER THAN 5 MINUTES WILL REQUIRE EXTRA DESTAINING TIME. 4.
TRANSFER the gel to a second small tray. COVER the gel with water. DESTAIN for at least 20
minutes with gentle shaking (longer periods will yield better results). Frequent changes of the
water will accelerate destaining. 5. Carefully REMOVE the gel from the destaining liquid.
VISUALIZE results using a white light visualization system. DNA will appear as dark blue bands on
a light blue background. 6. ESTIMATE the base pair length of each fragment by comparing the
distance each fragment traveled from the well to the bottom of the gel to the distance that each
Standard DNA Marker fragment migrated. For a more accurate size calculation you can use a
standard curve (see Appendix C). 7. Fill out the table below.