1 Describe and interpret the data in Figure 21 Describe .pdf
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Worksheet Information Sheet Part 1 Miz1 Transcription Fact.pdf
1 Describe and interpret the data in Figure 21 Describe .pdf
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1) Describe and interpret the data in Figure 2.1. Describe: What was measured? What are the patterns in the data? Interpret: What can you reasonably infer or conclude from the data? For example, this will often be a statement of the data suggest that X is necessary for Y. 2) What roles do the Mizm1 DNA sequence and the Miz-1 protein seem to be playing, according to the researchers results? Explain your reasoning using model of transcriptional initiation in eukaryotes in Figure 2.2 (below). Figure 2.1: Reporter plasmid. To study how specific DNA sequences help control gene expression, researchers create reporter plasmids. A reporter plasmid is a small piece of DNA engineered in the laboratory. Researchers cut out the DNA sequence(s) they want to study and paste it in front of a gene encoding a protein that is easily detected in the laboratory. In this case, the researchers use the luciferase gene, which encodes an enzyme that catalyzes a light-emitting reaction that is easily studied in the laboratory. The researchers created a reporter plasmid that had one of their DNA sequences of interest, which they called Mizm1 ("miz motif 1"), in front of a luciferase reporter gene (shown on the left). They also created a control plasmid that did not have the Mizm1 sequence, but did have the luciferase gene shown on the right). Figure 2.1. The researchers put either the reporter plasmid (pGL3e-Mizm1) or the control plasmid (pGL3ec) into cells from a human cell line (HeLa). They compared luciferase expression in cells with each plasmid that either did (Miz-1) or did not (EV) also contain the Miz-1 protein. eaux, B. L., Burow, D., Lockwood, S. H., Yu, A., Segal, D. J., & Knoepfler, P. S.Figure 2.2: Model of transcription initiation in eukaryotes..
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1 Describe and interpret the data in Figure 21 Describe .pdf
- 1) Describe and interpret the data in Figure 2.1. Describe: What was measured? What are the patterns in the data? Interpret: What can you reasonably infer or conclude from the data? For example, this will often be a statement of the data suggest that X is necessary for Y. 2) What roles do the Mizm1 DNA sequence and the Miz-1 protein seem to be playing, according to the researchers results? Explain your reasoning using model of transcriptional initiation in eukaryotes in Figure 2.2 (below). Figure 2.1: Reporter plasmid. To study how specific DNA sequences help control gene expression, researchers create reporter plasmids. A reporter plasmid is a small piece of DNA engineered in the laboratory. Researchers cut out the DNA sequence(s) they want to study and paste it in front of a gene encoding a protein that is easily detected in the laboratory. In this case, the researchers use the luciferase gene, which encodes an enzyme that catalyzes a light-emitting reaction that is easily studied in the laboratory. The researchers created a reporter plasmid that had one of their DNA sequences of interest, which they called Mizm1 ("miz motif 1"), in front of a luciferase reporter gene (shown on the left). They also created a control plasmid that did not have the Mizm1 sequence, but did have the luciferase gene shown on the right). Figure 2.1. The researchers put either the reporter plasmid (pGL3e-Mizm1) or the control plasmid (pGL3ec) into cells from a human cell line (HeLa). They compared luciferase expression in cells with each plasmid that either did (Miz-1) or did not (EV) also contain the Miz-1 protein. eaux, B. L., Burow, D., Lockwood, S. H., Yu, A., Segal, D. J., & Knoepfler, P. S.Figure 2.2: Model of transcription initiation in eukaryotes.