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In the example above, a segment of DNA is represented. Three possible PstI restriction sites exist. The numbers above the line represent the size of the respective fragments created after digestion with the restriction enzyme a) A band pattern is presented in lane 1 of the gel electrophoresis. Which if any of the three Pst I site is polymorphic in this case. b) Draw in lane 2 the band pattern if the PstI #1 site was destroyed in both copies of a diploid organism. c) Draw in lane 3 the band pattern if the PstI #2 site was destroyed in only one of the two copies of a diploid organism. d) Which change would explain the pattern in lane 4 (the 2 kb band is double)? Explain briefly.

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In the example above, a segment of DNA is represented. Three possible PstI restriction sites exist. The numbers above the line represent the size of the respective fragments created after digestion with the restriction enzyme a) A band pattern is presented in lane 1 of the gel electrophoresis. Which if any of the three Pst I site is polymorphic in this case. b) Draw in lane 2 the band pattern if the PstI #1 site was destroyed in both copies of a diploid organism. c) Draw in lane 3 the band pattern if the PstI #2 site was destroyed in only one of the two copies of a diploid organism. d) Which change would explain the pattern in lane 4 (the 2 kb band is double)? Explain briefly

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In the example above a segment of DNA is represented Three.pdf

  • 1. In the example above, a segment of DNA is represented. Three possible PstI restriction sites exist. The numbers above the line represent the size of the respective fragments created after digestion with the restriction enzyme a) A band pattern is presented in lane 1 of the gel electrophoresis. Which if any of the three Pst I site is polymorphic in this case. b) Draw in lane 2 the band pattern if the PstI #1 site was destroyed in both copies of a diploid organism. c) Draw in lane 3 the band pattern if the PstI #2 site was destroyed in only one of the two copies of a diploid organism. d) Which change would explain the pattern in lane 4 (the 2 kb band is double)? Explain briefly