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Learn about the ApeKI restriction enzyme recognition sequence and cleavage pattern

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a. Go to www.neb.com to learn more about this enzyme. b. Write below the (double-strand) recognition sequence for this enzyme, indicating the 5' end of each strand: c. On the recognition sequence shown above, insert two arrows to show where the enzyme cuts. d. This recognition sequence includes a "W". What does W mean? e. Write below the two "pieces" of DNA that result when this sequence has been cut with ApeKI. Show the 5' ends of each strand on each piece of DNA: f. Does this enzyme generate 5 ' overhangs, 3 ' overhangs, or does it generate blunt ends? enzyme (and how much better is that buffer than the other)?.

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a. Go to www.neb.com to learn more about this enzyme. b. Write below the (double-strand) recognition sequence for this enzyme, indicating the 5' end of each strand: c. On the recognition sequence shown above, insert two arrows to show where the enzyme cuts. d. This recognition sequence includes a "W". What does W mean? e. Write below the two "pieces" of DNA that result when this sequence has been cut with ApeKI. Show the 5' ends of each strand on each piece of DNA: f. Does this enzyme generate 5 ' overhangs, 3 ' overhangs, or does it generate blunt ends? enzyme (and how much better is that buffer than the other)?

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Learn about the ApeKI restriction enzyme recognition sequence and cleavage pattern

  • 1. a. Go to www.neb.com to learn more about this enzyme. b. Write below the (double-strand) recognition sequence for this enzyme, indicating the 5' end of each strand: c. On the recognition sequence shown above, insert two arrows to show where the enzyme cuts. d. This recognition sequence includes a "W". What does W mean? e. Write below the two "pieces" of DNA that result when this sequence has been cut with ApeKI. Show the 5' ends of each strand on each piece of DNA: f. Does this enzyme generate 5 ' overhangs, 3 ' overhangs, or does it generate blunt ends? enzyme (and how much better is that buffer than the other)?