Here is a summary by Andy Maloney and myself. This describes what we basically do in the lab and why we have so much fun doing it. This presentation was a blast to make and I look forward to doing many more. Credit: The song is "Missing You" by Trash80 from the album Icarus. It can be downloaded from 8bitpeoples.com.

1. A Tale of Two Labs Unzipping DNA and Molecular Motors

2. Microtubules

3. Kinesin

4. Slide preparation

5. Osmolytes

6. Part 2 DNA Unzipping

7. Unzipping DNA Components: Unzipping construct Optical Tweezers DNA to unzip Optional: proteins

8. We can unzip anything F F For plot below: Red Line – protein bound to DNA Black Line – naked DNA Black Dotted Line- predictions of protein locations Arrows – missing protein locations Unzipping can detect: Naked DNA “sequence” Changes to DNA sequence proteins bound to DNA And other possible structures

9. Shotgun DNA Mapping Algorithm matches unzipped random digestion fragments to library of simulated unzipping fragments Initial data is based on yeast genome Library of Simulated Curves Experimental Force Correct Match