1. MICROBIOLOGY FALL 2011

2. Office Hrs. MW 11-12; STC 239 Tue 10-1; NEWDL 312 Independence Rd. Laboratory M/W 12-3 Lecture MW 3-4:15

3. Syllabus

5. Dasek et al. 2000

7. Wild Animals Involved in MPXV Transmission (USA, 2003) Gambian Giant Rat (Crycetomys sp.) Prairie Dog (Cynomis sp.)

8. Ghana TX WI IL * IA 15/4 09/04 Entry and Distribution Route of Crycetomys sp . and Cynomys sp. Involved in MPXV Outbreak (USA, 2003) Source: MMWR 52 (23), CDC, 2003. 15/4

9. Index case in Marshfield, 26 May 2003: Disseminated lesions.

10. Distribution of MPXV Cases in the USA WI IL IN OH KS MO Number of cases = 87 WI = 38 IN = 24 IL = 19 OH = 4 KS = 1 MO = 1 Source: MMWR 52 (23), CDC, 2003.

13. Microbiology

14. Microbiology

23. DISEASE Occurrence of Disease

33. Toxins – Exo and Endotoxins

52. Prokaryotes / Eukaryotes Size of cell 1-10 u m diameter 10-100 u m diameter Nucleus No nuclear membrane True nucleus Membrane bound organelles absent present Flagella 2 protein building blocks complex

53. Prokaryotes / Eukaryotes Glycocalyx Slime layer Absent Cell Wall Usually present Complex When present, simple Plasma membrane No CHO and lack sterols Sterols and CHO present Cytoplasm No cytoskeleton cytoskeleton

54. Prokaryotes / Eukaryotes Ribosomes Small size 70s Large 80s; small 70s Chromosome Circular, lacks histones Linear, with histones Cell division Binary fission mitosis Sexual reproduction No meiosis meiosis

98. E.M Pili on E. coli – N. gonorrhea

151. COMPARISON OF GRM+VE /GRM-VE CELL WALLS

160. FLUID MOSAIC MEMBRANE Davison & Danelli

166. Bacterial Chromosome