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DEPARTMENT
OF
AGRIL.ENTOMOLOGY
GENERAL INFORMATION OF THE
STUDENT
NAME OF THE STUDENT :
Solunke Balram D
REGISTRATION NO : AKN-108/08
MASS PRODUCTION OF
Metarhizium anisopliae
(Deuteromycota; Hyphomycetes)
 An entomopathogenic fungus is fungal species that
can act as a parasite of insects and kills or seriously
disables them....
 Verticillium lecanii
 Beauveria bassiana
 Nomuraea rileyi
 Metarrhizium anisopliae
 Metarrhizium anisopliae is a widely distributed soil-
inhabiting fungus. The spore of M. anisopliae can be
formulated as...
rhinoceros beetle
Oryctes rhinoceros
The rhinoceros
beetle, Oryctes
rhinoceros is one of
the serious and
important pests of
coconut and has a
wide distribution...
• The adult beetle cause severe damage to the growing palms
by feeding on the tender fronds and crowns and resulting in
st...
 The chemical control measures
adopted against this pest are always
costly, tedious and have to be repeated
 Hence an ea...
The bottles are inoculated with 1 ml suspension
containing spores of the fungus with the help of a
sterile injection syrin...
The bottles are inoculated in a laminar flow chamber and
rested on flat surface for 2 days or till the surface of
medium i...
The flasks are incubated at room temperature. The spores can be harvested in a
fortnight.
From a clean uncontaminated moth...
Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)
Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)
Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)
Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)
Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)
Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)
Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)
Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)
Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)
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Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)

Types of Entomopathogenic Fungi like
Verticillium lecanii
Beauveria bassiana
Nomuraea rileyi
Metarrhizium anisopliae(detailed procedure of mass production in bio control lab)

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Mass production of  Metarhizium anisopliae (Deuteromycota; Hyphomycetes)

  1. 1. DEPARTMENT OF AGRIL.ENTOMOLOGY
  2. 2. GENERAL INFORMATION OF THE STUDENT NAME OF THE STUDENT : Solunke Balram D REGISTRATION NO : AKN-108/08
  3. 3. MASS PRODUCTION OF Metarhizium anisopliae (Deuteromycota; Hyphomycetes)
  4. 4.  An entomopathogenic fungus is fungal species that can act as a parasite of insects and kills or seriously disables them. These fungi usually attach to the external body surface of insects in the form of microscopic bodies (usually asexual, mitosporic spores also called conidia).
  5. 5.  Verticillium lecanii  Beauveria bassiana  Nomuraea rileyi  Metarrhizium anisopliae
  6. 6.  Metarrhizium anisopliae is a widely distributed soil- inhabiting fungus. The spore of M. anisopliae can be formulated as dust and sprayable formulation. It is used to control termites, mosquitoes, leafhopper, beetles etc.
  7. 7. rhinoceros beetle Oryctes rhinoceros
  8. 8. The rhinoceros beetle, Oryctes rhinoceros is one of the serious and important pests of coconut and has a wide distribution and persistent occurrence in all the coconut growing areas in the country. rhinoceros beetle
  9. 9. • The adult beetle cause severe damage to the growing palms by feeding on the tender fronds and crowns and resulting in stunting of the trees. The damage to the spathe results in the loss of nuts. Young seedlings are sometimes killed outright.
  10. 10.  The chemical control measures adopted against this pest are always costly, tedious and have to be repeated  Hence an easy method utilizing the safe and specific fungus, Metarhizium anisopliae for the management of this pest is aimed at.
  11. 11. The bottles are inoculated with 1 ml suspension containing spores of the fungus with the help of a sterile injection syringe. Before inserting the needle within the sterile bottles for drawing spore suspension for inoculation, the needle of the syringes and collar region of the bottles are flamed (Bunsen burner). Coconut water (40 ml) contained in 375 ml side wise flat liquor bottles plugged with cotton plug are sterilized in batches of 9-10 bottles in 12 liters pressure cooker for 20 minutes at 15 psi.
  12. 12. The bottles are inoculated in a laminar flow chamber and rested on flat surface for 2 days or till the surface of medium is fully covered by the olive green sporulated fungus. The whole culture is crushed thoroughly in an ordinary mixer and used in the field. From a single average size coconut 5-6 bottles of cultures can be made.
  13. 13. The flasks are incubated at room temperature. The spores can be harvested in a fortnight. From a clean uncontaminated mother culture in slant loopful quantities of M. anisopliae spores are transferred aseptically. The flasks are allowed to cool and taken to laminar flow chamber for inoculation The conical flasks are plugged with cotton and autoclaved for 20 min at 15 psi. Carrot cut into small pieces (40 g) is washed in potable water and transferred to conical flask (250 ml) and 15 ml of distilled water is added.

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Types of Entomopathogenic Fungi like Verticillium lecanii Beauveria bassiana Nomuraea rileyi Metarrhizium anisopliae(detailed procedure of mass production in bio control lab)

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