This document describes various next generation sequencing products from BioChain including: 1) CancerSeq characterized cancer samples for NGS with genetic information on 48 cancer genes. 2) mRNA and small RNA sample prep kits for NGS with directional libraries and compatibility with FFPE samples. 3) NGS testing services including extraction, library prep, sequencing and data analysis using their collection of over 300,000 frozen and FFPE blocks.

1. Sample Preparation for
Diagnostics & Life Science
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2. Next Generation
Sequencing Products

3. CancerSeq-Characterized Cancer FFPE tissue samples
by Next Generation Sequencing (NGS)
Sample and Services for Next Generation Sequencing
Cancer Gene Panel-NGS Testing Service
RapidSeq-Directonal mRNA Sample Prep Kit for NGS
RapidSeq-Small RNA Sample Prep Kit for NGS
Sample Preparation for
Diagnostics & Life Science
www.biochain.com
1-888-762-2568 (Main)
order@biochain.com (Order)

4. BioChain’s CancerSeq™
genetically characterized FFPE blocks give you
and your data more power
Blocks and Slides available for:
- Breast Cancers
- Colon Cancers
- Lung Cancers
- Melanomas
- Other Cancers (via request)*
Cancer Gene Panel Includes:
The Illumina TruSeq® Amplicon - Cancer Panel
(TSACP) to characterize 48 cancer genes,
including BRAF, KRAS, EGFR, ALK, and Tp53.
Features
- Tissue from a wide variety of sources, Whole FFPE blocks, slides, and DNA
- Suitable for immunohistochemistry, in situ hybridization assays,
and molecular applications
ABL1 ERBB4 JAK2 PIK3CA
AKT1 FBXW7 JAK3 PTEN
ALK FGFR1 KDR PTPN11
APC FGFR2 KIT RB1
ATM FGFR3 KRAS RET
BRAF FLT3 MET SMAD4
CDH1 GNA11 MLH1 SMARCB1
CDKN2A GNAQ MPL SMO
CSF1R GNAS NOTCH1 SRC
CTNNB1 HNF1A NPM1 STK11
EGFR HRAS NRAS TP53
ERBB2 IDH1 PDGFRA VHL
CATALOG # PRODUCT NAME SIZE
T2235086-SB CancerSeq™ Paraffin Tissue Tumor Block: Breast 1 Block
T2235090-SB CancerSeq™ Paraffin Tissue Tumor Block: Colon 1 Block
T2235152-SB CancerSeq™ Paraffin Tissue Tumor Block: Lung 1 Block
T2235218A-SB CancerSeq™ Paraffin Tissue Tumor Block: Skin Melanoma 1 Block
- Make sense out of ambiguous IHC results by knowing the genetics
- Buy just the blocks with the right genetic background
- Search our databases for blocks with novel mutations
- Take advantage of NGS without expensive infrastructure

5. NGS Samples and
Services
As a provider of tissues and nucleic acids derived from them,
BioChain is uniquely positioned to extract DNA and RNA from the
most challenging tissues, and perform re-sequencing and RNAseq.
Tissues:
We have over 300,000 Frozen and FFPE blocks to collect DNA and RNA
from. You may also contract with us to procure rare materials for your
sequencing project.
Extraction:
We have provided high quality DNA and RNA from the most challenging
tissues including FFPE blocks, cartilage, and brain for over 15 years.
Library preparation:
We are Experienced with all Illumina library preparation kits as well as
our proprietary RapidSeqTM small RNA library prep kit.
Illumina MiSeq NGS:
With special expertise in RNAseq and resequencing with Trueseq
Custom and Cancer Amplicon panels, we handle a wide variety of
sequencing projects.
Data analysis and consultation:
We can help with experimental design through variant calling and anno-
tation.

6. Cancer Driver Gene Panel
NGS Testing Service
One - Step Shop Cancer Sequencing Test
Services from Beginning to End
NGS Leading Platform
-
- Greater than 35 kb of cancer
consensus targets including BRAF,
KRAS & EGFR
- 212 amplicons in one tube; 48 genes
Unrivaled Multiplexing
- Up to 96 sample pooling on MiSeq
- >90% specificity and uniformity
- Detect low frequency variants (<5%)
Unparalleled Workflow
- FFPE-enabled with sample QC Kit
- Automated paired end sequencing
with MiSeq
Industry Standard Delivery
- Cost-effective solution
- Quick turn-around time
ABL1 EGFR GNAS MLH1 RET
AKT1 ERBB2 HNF1A MPL SMAD4
ALK ERBB4 HRAS NOTCH1 SMARCB1
APC FBXW7 IDH1 NPM1 SMO
ATM FGFR1 JAK2 NRAS SRC
BRAF FGFR2 JAK3 PDGFRA STK11
CDH1 FGFR3 KDR PIK3CA TP53
CDKN2A FLT3 KIT PTEN VHL
CSF1R GNA11 KRAS PTPN11
CTNNB1 GNAQ MET RB1
48 Cancer Biomarker
AATTCCGGCCGATCAGGGG
TTTACACCAATGGGACCATTAC
CCAAAGGCCTTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGG
AATTCCGGCCGATCAGGGGTTTACACCAATGGGACCATTACC
CAAAGGCC
TTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGAATTCCGGCCGATCAGGGGTTTACACCAAT
GGTTTACACCAATGGGACCATTACCAAC

7. Directional mRNA
Sample Prep Kit
Sample Prep System for mRNA Sequencing
Features
• Low RNA Input
Requires as little as 50 ng of total RNA input
• Directional
Increased useful number of reads
• < 1 Hour Hands-on Time
Streamlined workflow
Library construction in < 7 hours
• All-in-One Kit
Includes all necessary enzymes and plastics
• FFPE RNA Compatible
High Quality Libraries without
Artifacts or Contaminations
Bioanalyzer Trace of Constructed Library
of mRNA from corn
Description
The RapidSeq Directional mRNA Sample Prep kit provides strand-specific cDNA synthesis
with reduced costs and increased sensitivity.
Directionality Assessment
Comparison with TruSeq in Prokaryotic Cells
mRNA Input (ng) Sample Type % Sense
100 (Competitor I Kit) FFPEmRNA (1 year) 97.85%
100 (RapidSeq Kit) FFPEmRNA (1 year) 97.88%
500 (Competitor I Kit) FFPEmRNA (10 years) 97.59%
500 (RapidSeq Kit) FFPEmRNA (10 years) 97.57%
Parameter TruSeq RapidSeq
Coverage Rate 44.00% 65.00%
rRNA Contamination with 23s, 16s, and 5s
removal
2.70% 1.50%
tRNA contamination 64.00% 57.00%
Unknown sequence percentage 5.00% <1%
Directional Percentage 74% 77.50%
Average Quality Score (range from 0 to 40,
with 40 being the highest quality)
26 37
CATALOG # PRODUCT NAME
KS073012 RapidSeq High Yield Directional mRNA Sample Prep Kit - Without Aligner
KS073012 I-IV RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligners 1-48
K2012008 MagSeq mRNA Purification Kit
AATTCCGGCCGATCAGGGGTTTACACCAATGGGACCATTACCCAAAGGCCTTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGGTGGCCATGGAACCTTGGAC
AATTCCGGCCGATCAGGGGTTTACACCAATGGGACCATTACCCAAAGGCCTTAACCAAGGCCTTAATTCAGACTTCCAGGCCCCAGGTGGCCATGGAACCTTGGACCCATTTAGAACAG

8. Small RNA Sample
Prep Kit Everything you need in one box
Applications
• Small RNA discovery and quantification
• miRNA expression profiling
• miRNA related functional assessments
Description
The RapidSeq Small RNA Sample Prep Kit
is a cost effective and reliable solution for small
RNA library preparation using Illumina’s
sequencing platform. Our reagents are master
mixed for optimal performance and simple
experiment setup, which minimizes human error.
RapidSeq can be used for a variety of studies
involving small RNA libraries.
Construct Pure Libraries
with no Artifacts or Contamination
Bioanalyzer Trace of FFPE microRNA Library
Highly Consistent and
Reproducible Results
Normalized R2 Plot of miRNA counts
from Two Replicate Libraries
CATALOG # PRODUCT NAME
KS071012-I RapidSeq Small RNA Sample Prep Kit - With Aligner 1-12
KS071012-II RapidSeq Small RNA Sample Prep Kit - With Aligner 13-24
KS071012-III RapidSeq Small RNA Sample Prep Kit - With Aligner 25-36
KS071012-IV RapidSeq Small RNA Sample Prep Kit - With Aligner 37-48
Save time
Less than 1 hour hands-on (7 hours with incubations).
Avoid purchasing headaches
Includes all necessary enzymes and plastics.
Don’t worry about RNA quantity
Good results with as little as 1 ng or as much as 10 µg of total RNA.
Have FFPE RNA? No problem!
FFPE RNA compatible

9. NGS Related
Products
T2235086-SB CancerSeq Parafin Tissue Tumor Block: Breast 1 Block
T2235090-SB CancerSeq Parafin Tissue Tumor Block: Colon 1 Block
T2235152-SB CancerSeq Parafin Tissue Tumor Block: Lung 1 Block
T2235218A-SB CancerSeq Parafin Tissue Tumor Block: Skin Melanoma 1 Block
KS073012-I RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 1-12 1 Kit
KS073012-II RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 13-24 1 Kit
KS073012-III RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 25-36 1 Kit
KS073012-IV RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 37-48 1 Kit
KS073012 RapidSeq High Yield Directional mRNA Sample Prep Kit - Without Aligner 1 Kit
KS073012-T
RapidSeq High Yield Directional mRNA Sample Prep Kit, Trial version enough
for 2 rxn. Customer select aligners
1 Kit
KS074012-I RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 1-12 1 Kit
KS074012-II RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 13-24 1 Kit
KS074012-III RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 25-36 1 Kit
KS074012-IV RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 37-48 1 Kit
KS074012 RapidSeq High Yield Small RNA Sample Prep Kit - Without Aligners 1 Kit
KS074012-T
RapidSeq High Yield Small RNA Sample Prep Kit, Trial version enough for 2 rxn.
Customer select aligners
1 Kit
KS075012-I RapidSeq MasterMix Directional mRNA Sample Prep Kit - With Aligner 1-12 1 Kit
KS075012-II RapidSeq MasterMix Directional mRNA Sample Prep Kit - With Aligner 13-24 1 Kit
KS075012-III RapidSeq MasterMix Directional mRNA Sample Prep Kit - With Aligner 25-36 1 Kit
KS075012-IV RapidSeq MasterMix Directional mRNA Sample Prep Kit - With Aligner 37-48 1 Kit
KS075012 RapidSeq Mastermix Directional mRNA Sample Prep Kit - Without Aligners 1 kit
KS071012-I RapidSeq MasterMix Small RNA Sample Prep Kit - With Aligner 1-12 1 Kit
KS071012-II RapidSeq MasterMix Small RNA Sample Prep Kit - With Aligner 13-24 1 Kit
KS071012-III RapidSeq MasterMix Small RNA Sample Prep Kit - With Aligner 25-36 1 Kit
KS071012-IV RapidSeq MasterMix Small RNA Sample Prep Kit - With Aligner 37-48 1 Kit
KS071012 RapidSeq MasterMix Small RNA Sample Prep Kit - Without Aligner 1 Kit
CATALOG # DESCRIPTION UNITS

10. Other Related
Products
D1B34999-G02 Control Genomic DNA - Bovine Female 100 µg
D1B34999-G01 Control Genomic DNA - Bovine Male 100 µg
D1C34999-G02 Control Genomic DNA - Chicken Female 100 µg
D1C34999-G01 Control Genomic DNA - Chicken Male 100 µg
D1534999-Cy-G02 Control Genomic DNA - Cynomolgus Monkey Female 100 µg
D1534999-Cy-G01 Control Genomic DNA - Cynomolgus Monkey Male 100 µg
D1734999-G02 Control Genomic DNA - Dog Female 100 µg
D1734999-G01 Control Genomic DNA - Dog Male 100 µg
D1G34999-G02 Control Genomic DNA - Guinea Pig Female 100 µg
D1G34999-G01 Control Genomic DNA - Guinea Pig Male 100 µg
D1H34999-G02 Control Genomic DNA - Hamster Female 100 µg
D1H34999-G01 Control Genomic DNA - Hamster Male 100 µg
D1234999-G02 Control Genomic DNA - Human Female 100 µg
D1234999-G01 Control Genomic DNA - Human Male 100 µg
D1334999-G02 Control Genomic DNA - Mouse Female 100 µg
D1334999-G01 Control Genomic DNA - Mouse Male 100 µg
D1934999-G02 Control Genomic DNA - Porcine Female 100 µg
D1934999-G01 Control Genomic DNA - Porcine Male 100 µg
D1834999-G02 Control Genomic DNA - Rabbit Female 100 µg
D1834999-G01 Control Genomic DNA - Rabbit Male 100 µg
D1434999-G02 Control Genomic DNA - Rat Female 100 µg
D1434999-G01 Control Genomic DNA - Rat Male 100 µg
D1534999-G02 Control Genomic DNA - Rhesus Monkey Female 100 µg
D1534999-G01 Control Genomic DNA - Rhesus Monkey Male 100 µg
R1234010-50 Total RNA - Human Adult Normal Tissue: Bladder 50 µg
R1234035-50 Total RNA - Human Adult Normal Tissue: Brain 50 µg
R1234086-50 Total RNA - Human Adult Normal Tissue: Breast 50 µg
R1234090-50 Total RNA - Human Adult Normal Tissue: Colon 50 µg
R1234090-P Total RNA - Human Adult Normal Tissue 5 Donor Pool: Colon 50 µg
R1234122-50 Total RNA - Human Adult Normal Tissue: Heart 50 µg
R1234142-50 Total RNA - Human Adult Normal Tissue: Kidney 50 µg
R1235010-10 Total RNA - Human Tumor Tissue: Bladder 10 µg
R1235023-10 Total RNA - Human Tumor Tissue: Bone 10 µg
R1235086-50 Total RNA - Human Tumor Tissue: Breast 50 µg
R1235090-50 Total RNA - Human Tumor Tissue: Colon 50 µg
R1235142-50 Total RNA - Human Tumor Tissue: Kidney 50 µg
R1235149-50 Total RNA - Human Tumor Tissue: Liver 50 µg
Control Genomic DNA
Total RNA

12. Sample Preparation for Diagnostics & Life Science
www.biochain.com
1-888-762-2568 (Main)
order@biochain.com (Order)