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CoolCell    TM




Cell Freezing Container




                          1	
  
CoolCell

  An alcohol-free cell freezing
   container


                                            CoolCell



  Replaces “Mr. Frosty”
    An alcohol-filled freezing container


                                            Mr. Frosty


                                   2	
  
Background
  Most cell culture researchers freeze cells in a
      o
   -80 C freezer prior to storing in liquid nitrogen

                                                   o
  They need a slow freeze rate of -1 C/minute

  “Mr. Frosty” is commonly used now
     It uses 100% iso-propylalcohol (IPA) to control the freezing
                              o
      rate to approximately -1 C/minute
  “CoolCell” controls the freezing rate MORE
   CONSISTENTLY
     with no alcohol – no fluids – no maintenance – no cost

                                    3	
  
“Mr. Frosty”
  The alcohol in “Mr. Frosty” must be replaced
   every 5 uses in order to keep the freeze rate
                       o
   near the desired -1 C/minute. This means…..
    you have to keep track of how many times you use it (hassle!)
    you have to purchase alcohol (expense!)
    you have to dispose used alcohol (hassle, smell & expense!)


  More importantly, it also means that the alcohol
   level changes every time it is used
    “Mr. Frosty” results in different freezing profile if not maintained
     properly
    It is a heat sucker “Caution” can ruin your samples/probes in
     freezer
                                    4	
  
CoolCell

                                                               o
  CoolCell delivers a very consistent -1 C/minute
   freeze rate without alcohol
    Freezing profiles are extremely consistent every time
    Less variability and high repeatability in your research




                        Fusion time and cooling profiles are
                        highly reproducible from run to run


                                          5	
  
CoolCell
  CoolCell outperforms “Mr. Frosty” in cell viability
     Researchers care most about cell viability (i.e. how many cells
      are still viable after they are thawed out at a later date)




    In primary human cells, CoolCell significantly outperforms



                                  6
Additional CoolCell Advantages
  No on-going cost – COST SAVINGS!
       “Mr. Frosty” costs approx. $350/year for alcohol purchase & disposal


  Lid is easy to open – even when frozen
       “Mr. Frosty” screw cap is very difficult to open when frozen


  Not cold to the touch and not “frosty” when frozen
       “Mr. Frosty” requires special gloves to handle and is very slippery due to frost/ice
        on exterior


  Ready to use again after only 5 minutes
       “Mr. Frosty” requires about 1.5 hours for alcohol to thaw before it can be used
        again



                                            7	
  
CoolCell vs “Mr. Frosty”
                        CoolCell                                             “Mr. Frosty”
  No alcohol                                                Requires 250ml alcohol
  • No hazardous fluids                                     • Replace alcohol every 5 uses (250ml)
  • Solid core and insulation design                        • Keep track of # of uses
  • No pre-cooling required                                 • Alcohol degrades slightly each time it’s used
                                                            • Pre-cool alcohol in refrigerator
  Very consistent -1oC/min rate                             Inconsistent freeze rate
  • All vials have uniform freeze rate every time           • Each run is slightly different due to alcohol
  • Radially symmetric – design enables consistency         degradation
                                                            • Two circles of wells – different freeze rates for each
                                                            circle
  Cost savings                                              ~$338/year maintenance per unit
  • No maintenance cost                                     • Change alcohol weekly (~$6/wk)
  • Pays for itself quickly                                 • Dispose haz waste (~$0.75/wk)
                                                            • 10 units = $3,380 to use EVERY YEAR
  Lid opens easily                                          Screw cap very difficult to remove when frozen
  • Not cold to the touch when removing from the -80C       • Protective gloves required when handling frozen unit
  freezer
  Better cell viability                                     Good cell viability


  Ready to use again in 5 minutes                           Takes >1 hour for device to return to room
                                                            temp for re-use

  Low impact on freezer                                     Large thermal mass impacts local freezer area
  • 1/3 the heat impact on freezer compared to alcohol-     • Large heat capacity removed from alcohol impacts
  filled units                                              nearby samples



                                                          8	
  
CoolCell video




                 Click here




                      9	
  
Are you a CoolCustomer?




                  10	
  
Thank you


           Michelle Nemits
VP, Sales and Business Development
        Cell: 415-336-4415

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BioCision CoolCell

  • 1. CoolCell TM Cell Freezing Container 1  
  • 2. CoolCell   An alcohol-free cell freezing container CoolCell   Replaces “Mr. Frosty”   An alcohol-filled freezing container Mr. Frosty 2  
  • 3. Background   Most cell culture researchers freeze cells in a o -80 C freezer prior to storing in liquid nitrogen o   They need a slow freeze rate of -1 C/minute   “Mr. Frosty” is commonly used now   It uses 100% iso-propylalcohol (IPA) to control the freezing o rate to approximately -1 C/minute   “CoolCell” controls the freezing rate MORE CONSISTENTLY   with no alcohol – no fluids – no maintenance – no cost 3  
  • 4. “Mr. Frosty”   The alcohol in “Mr. Frosty” must be replaced every 5 uses in order to keep the freeze rate o near the desired -1 C/minute. This means…..   you have to keep track of how many times you use it (hassle!)   you have to purchase alcohol (expense!)   you have to dispose used alcohol (hassle, smell & expense!)   More importantly, it also means that the alcohol level changes every time it is used   “Mr. Frosty” results in different freezing profile if not maintained properly   It is a heat sucker “Caution” can ruin your samples/probes in freezer 4  
  • 5. CoolCell o   CoolCell delivers a very consistent -1 C/minute freeze rate without alcohol   Freezing profiles are extremely consistent every time   Less variability and high repeatability in your research Fusion time and cooling profiles are highly reproducible from run to run 5  
  • 6. CoolCell   CoolCell outperforms “Mr. Frosty” in cell viability   Researchers care most about cell viability (i.e. how many cells are still viable after they are thawed out at a later date) In primary human cells, CoolCell significantly outperforms 6
  • 7. Additional CoolCell Advantages   No on-going cost – COST SAVINGS!   “Mr. Frosty” costs approx. $350/year for alcohol purchase & disposal   Lid is easy to open – even when frozen   “Mr. Frosty” screw cap is very difficult to open when frozen   Not cold to the touch and not “frosty” when frozen   “Mr. Frosty” requires special gloves to handle and is very slippery due to frost/ice on exterior   Ready to use again after only 5 minutes   “Mr. Frosty” requires about 1.5 hours for alcohol to thaw before it can be used again 7  
  • 8. CoolCell vs “Mr. Frosty” CoolCell “Mr. Frosty” No alcohol Requires 250ml alcohol • No hazardous fluids • Replace alcohol every 5 uses (250ml) • Solid core and insulation design • Keep track of # of uses • No pre-cooling required • Alcohol degrades slightly each time it’s used • Pre-cool alcohol in refrigerator Very consistent -1oC/min rate Inconsistent freeze rate • All vials have uniform freeze rate every time • Each run is slightly different due to alcohol • Radially symmetric – design enables consistency degradation • Two circles of wells – different freeze rates for each circle Cost savings ~$338/year maintenance per unit • No maintenance cost • Change alcohol weekly (~$6/wk) • Pays for itself quickly • Dispose haz waste (~$0.75/wk) • 10 units = $3,380 to use EVERY YEAR Lid opens easily Screw cap very difficult to remove when frozen • Not cold to the touch when removing from the -80C • Protective gloves required when handling frozen unit freezer Better cell viability Good cell viability Ready to use again in 5 minutes Takes >1 hour for device to return to room temp for re-use Low impact on freezer Large thermal mass impacts local freezer area • 1/3 the heat impact on freezer compared to alcohol- • Large heat capacity removed from alcohol impacts filled units nearby samples 8  
  • 9. CoolCell video Click here 9  
  • 10. Are you a CoolCustomer? 10  
  • 11. Thank you Michelle Nemits VP, Sales and Business Development Cell: 415-336-4415