Common Use Data System For Food Animal Genetics In Taiwan
1. Common-use Data System for Food
Animal Genetics in Taiwan
Ming-Che Wu
Taiwan Livestock Research Institute
2. A reservoir of genetic adaptability acts a buffer
against potentially harmful environmental and
economic change.
Food security and mileage (Sustainability)
3. System for Food Animal Genetics
in Taiwan
Phase I (1987~1992):
Conservation farms
●
Conservation farms
Phase II(1993~1998):
● Web site www.angrin.tlri.gov.tw
Web site
Phase III(1999~2004):
Cryopreservation Bank
●
Conservation center
Phase IV(2005~2008):
Molecular techniques
●
Research and Utilization
Genetic resources Phase V(2009~2012):
●
exchange Global Bioidentification System
4. Common-use
• DNA in food chain
• Biogenetic banks in
supporting scientific
research
• Genomic selection on
farm level
• Web-based barcode
system with food mileage
11. BioUtilization
Conservation Biodiversity
Species
Species
verification
Value add
Selection Demand
New
breed/line
Tracking
Export
Genetic
improvement
Genomic breeding
12. (source:FAO, 2007)
Global Movement of AnGR
(milk)
(pork)
(pork)
(pork)
(milk)
(milk)
(chicken meat)
(chicken egg)
(fur sheep)
13. Biogenetic resources are parts of our life.
Genetic diversity is threatened by
- genetic erosion
- habitat loss
- social value sinking
- economic setback
- food print mileage
From Farm to DNA Bank
14. Genetic
Banking
Living form
Cell/tissue
DNA/RNA/mRNA/
cDNA/protein
15. Development of miniature pig for biomedical research in Taiwan
Ming-Che Wu, PhD
Taiwan Livestock Research Institute
www.angrin.tlri.gov.tw
mcwu@mail.tlri.gov.tw
Taiwan
Lanyu
16.
17. Development of miniature pig for biomedical research in Taiwan
Ming-Che Wu, PhD
Taiwan Livestock Research Institute
www.angrin.tlri.gov.tw
mcwu@mail.tlri.gov.tw
Lanyu 200
Hybrid
Hybrid
Inbreeding Duroc
GPI-CRC-PGD Genotyped Mitsae (Lanyu 50)
Spotty Lanyu
Line (Lanyu 300) (Lanyu 100) (50% Lanyu + 50% Duroc)
18. Mitasi minipig was developed from sib-mating on hybrid of Duroc
and Lanyu breed.
1989
1990
1991
1992
1993
1994
1995
(Lanyu 50)
50
26. •K Series - Liquid nitrogen storage with controllable
temperatures between (-100°C to -196°C)
Small LN Tank
-185℃ Vapor
storage
•designed for large
vial capacities up
to 6000 vials (2 ml
vial) in box type
racks.
-80℃ Freezer
27. Bio-security
-185℃ Vapor
storage
•designed for large
vial capacities up to
6000 vials (2 ml vial) in
box type racks.
28. Gene Banking
DNA Banking
2007 Living form/Cell/Tissue
DNA/RNA/mRNA/cDNA/Protein
Cattle 6180
Water Buffalo 58
Goat 3127
Oocyte
Sheep 14
Horse 14
Rabbit 88
Swine 25129
Chicken 11650
Muscovy 151
Embryo
Duck 689
Hybrid Duck 128
Goose 431
Turkey 14
Black Swan 135
Quail 50
Total 47858
31. Impact of common-use breeding data on production efficiency
2.20
Duroc Landrace Yorkshire
2.15 Feed efficiency(Feed/Gain) from 40 to 110 kg of body weight
2.10
2.05
2.00
2000 2001 2002 2003 2004 2005 2006 2007
Birth year of boar
41. Regulation for genomic breeding - Animal Industry Act Article 17
The competent authority may dispatch inspector to examine or test the breeding
flock, breeding stock, facilities, pedigree registry and related records of breeders, and
the breeders shall not evade, interfere with or object to such examination or testing.
Breeding flock or breeding stock found to have contracted notified disease or have
hereditary disease during the aforesaid examination or testing shall be banned from
breeding. The aforesaid inspectors shall present their identification when carrying out
their duty.
Pig Cow Goat Chicken Duck Goose Deer Rabbit
Hal-1843 BLAD G6S HSP70 PRL PRL IGF2 GPI
ESR DUMP IGF2 PRLR ESR ESR XY PGD
Gene
HFABP-H CITR mtDN PRL ZW ZW D5FAD XY
A
HFABP-ad CVM ESR mtDNA mtDNA mtDNA IGF2
IGF2-In3 XY mtDNA mtDNA
IGF2-In7 GH ZW
D5FAD mtDNA
D-Loop
SW18
SW1943
SW2588
PRLR
42. DNA barcoding for pigs
DNA
Hal-1843 gene for stress (AB), ESR gene for prolificacy
(MN) and 16,613bp mtDNA for maternal origin,
especially on D-loop region (1,175bp) of 29 SNPs and
UTRn (tacacgtgcg 10bp)17~27 in various breeds of pigs.
AB+MN+18SNP+UTR+11SNP
mtDNA D-Loop
AAMNcaattgctcgtttctcag24tgctcgaaacc
A0021-02 Lanyu
T0596-01 Taoyuan AAMMcaattgcttgttttccag24tgctcgaaact
L0074-10 Landrace AAMNttgccattcaccctccaa27tgcttaaaatt
Y0150-03 Yorkshire AAMNcaattgcctgttttccag25tccccgaaacc
AANNttgccattcaccctccaa26tgcttaaaatc
D0167-05 Duroc
44. Common-use
• DNA in food chain
• Biogenetic banks in
supporting scientific
research
• Genomic selection on
farm level
• Web-based barcode
system with food mileage
45. Acknowledgements • Wu Lab
– Mr. Yun-Yu Lai
– Dr. Ren-Bao Liaw
• –
Funding Sponsors Dr. Yuchia Huang
–
– COA Dr. Ter-Yu Lin
–
– TLRI Ms. Hsiu-Rong Tsai
– Mr. Hsenn-Ben Chu
– NAIF
• Collaborators
– Prof. Hsiu-Luan Chang
– Dr. Lih-Ren Chen
– Prof. Feng-Pang Cheng
– Prof. Larry Schook, UIUC
– Prof. Harris Lewin, UIUC
– Dr. Le Thi Thuy, Vietnam
– Prof. Rangsun Parnpai, Thailand
– Dr. Libertado C. Cruz, Philippines
– Prof. Chis Maxwell, Australia
– Prof. Locksley E. McGann, Canada
– Prof. Hoon Taek Lee, Korea