Presented at Nordic Green II conference, April 2010

1. BioIsoprene™: A C5 Platform for
Production of Branched Chain
Hydrocarbons for use in Biochemical
and Biofuel Applications
Gregory Bohlmann
April 28, 2010
Nordic Green II
Conference
Palo Alto, CA

2. Genencor at a Glance….
We put Biotechnology to work… developing & Industrial
producing enzymes for industrial applications. Enzymes
Animal Feed Enzymes
Cellulosic Enzymes
History traced to 1982
Food Enzymes
May 2005: Division of Danisco Fabric & Household
Care
Industrial Biotechnology Focus Grain Processing
Textiles Enzymes
Over 250 commercial products
Proven innovator: ~3,500+ owned &
licensed patents and patent Biochemicals
Lysine
applications Threonine
> $800 MM USD annual turnover 1,3 Propanediol
Tryptophan
Indigo
~ 1,200 employees worldwide H CH3 Biotin
C C Ascorbic Acid
> 5 million liters of fermentation 1,3 Propanediol
H2C CH2
capacity in 14 plants around the world BioIsoprene™
BioIsoprene™
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3. Isoprene: a Chemist’s View
2-methyl-1,3-butadiene (C5H8)
• Colorless liquid
• Volatile (boiling point 34oC)
• Insoluble in water
Forms dimers and trimers Readily polymerizes
[ [
[
[
trans-1,4-polymer cis-1,4-polymer
(synthetic rubber)
[
[
[
[
1,2-polymer 1,2-polymer
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4. BioIsoprene™ Molecule:
Genencor’s Unique C5 Platform
Tires
Biochemicals
Purification
Adhesives
High Purity
BioIsoprene™ monomer
Specialty Polymers
H CH3
C C
H2C CH2
Isoprene
Monomer Gasoline
Renewable Microbial Strain Large Scale Gas Phase
C10 Isomers
Feedstocks Development Fermentation Recovery
C15 Isomers
+
+ Other Fuels & Blends
Diesel
Biofuels BioIsoFuel™ Examples Jet Fuel
Chemical
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5. We add to the biofuels sector a new way to
produce a hydrocarbon blend stock.
MeOH
Methane Syngas Cellulosic
EtOH EtOH
C1 Dimethyl
ether
bio-H2 C2
Long C-chain
Families
Biopropane
Clean
Diesel Fatty Acids C3
Propanol
Algae-based
Biofuels
Biobutanol
>C6 C4
Isobutylene
to fuel
Isobutanol to
C6 C5 isobutylene
Furfural derivatives
C5 Isoprene Monomer
(Hemiterpene) Platform
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C20 Di- and higher terpenes
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6. BioIsoprene™ derived C10 & C15 fuels
compare favorably to gasoline and diesel:
Gasoline Comparison Diesel Comparison
Cellulosic BioIsoFuelTM BioIsoFuelTM
Variable Gasoline BioButanol #2 Diesel BioDiesel
Ethanol C10 C15
Energy Content
[Low heating value 116,090 76,330 99,800 131,600 128,450 119,550 125,200
(BTU/gallon)]
Energy Content
100.0% 65.8% 86.0% 113.4% 100.0% 93.1% 97.5%
(Comparison)
Target Blending
100% 10% 15% 50% 100% 100% 100%
(V/V)
Requirement for
New No Yes No No No No No
Infrastructure
Feed Sugar / Sugar /
Crude Oil Biomass Biomass Crude Oil Biomass
Stock Biomass Biomass
C10 & C15 BioIsoFuelsTM:
• Pure hydrocarbons
• High energy content compared to other biofuels
• Value in market linked to energy content
• ~80% reduction in GHG emissions compared to traditional petroleum-based fuels
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7. BioIsoprene™ Enables Biorefinery
Approach:
BioIsoprene™ process, developed jointly by
Genencor and The Goodyear Tire & Rubber Company
BioIsoprene™ Production:
• rDNA
• Biomass-based
• Gas Phase Product
Establishes C5 Platform (5-carbon molecule) to
produce valuable products:
• A Business Platform Chemical
• Biochemicals: Tires; Adhesives; Specialty
Elastomers
• Biofuels: Liquid transportation Fuels; Jet Fuels
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