2. • Ozone is the tri-atoms allotrope form of the oxygen, it is a highly reactive
gas and it is also very oxidant;
• It is however also very unstable ant it tends to return to its molecular
form ,which is more stable, from which it is generated, that is oxygen in a
variable time (about 30-40minutes ) depending on the concentration , the
temperature and the air characteristics (humidity, the presence of
volatile organic substances etc..)
• Ozone can be produced by different methods. The most effective involves
the passage of air to oxygen through an electric field of appropriate
voltage.
• The electrical discharge excites the electrons of O2 allowing the
separation of the atoms , which can combine with others so forming
ozone
What is ozone?1
3. 2
Ozone is one of the most oxidizing compounds ,( its power is up to 10 times higher if
compared to other products containing chlorine), for this reason it has is a very broad
antimicrobial power , it is active against bacteria, fungus, viruses, protozoa and bacterial
spores.
Thanks to its high oxidizing power , it is capable of damaging the membrane and the cells
wall; microorganisms die for lysis or disintegration of the cellular compounds.
This mechanism is much more rapid than the one caused by other disinfectants, which
must first of all penetrate the cell membrane before being able to start their action.
Also for its action against spores , the lesions of the outer coating of the spores , which
protect the internal structures , would be essential.
To all these advantages , another very important one must be added:
Ozone does not leave any residue, smell or taste ,neither in water nor on surfaces with
which it comes in contact, not even on those of natural origin or not treated such as
wood ; it can so be used in gaseous or aqueous form without rinsing
Why using ozone?
5. La soluzione OZONO: ecco i vantaggi4
No cost due to the use of drinking water for the disinfection process
No cost of disinfectants
No environmental impact
Reduction of the energy costs, because the process does not
move water but only air ,so the pumps consume less
The personnel must not be present during the process and
must not prepare any solution or other disinfectants. No loss
of time to rinse
1
2
3
4
5
7. BRETTANOMYCES a study6
Tab. 1 Risultati nelle barrique ad un mese dal primo trattamento
Posizione
prelievo
Barrique 1
trattata (UFC/
dm2)
Barrique 2
non trattata
(UFC/dm2)
Barrique 3
testimone
(UFC/dm2)
A 78000 480000 0
B 18000 600000 0
C 680000 1550000 0
Barrique piuttosto contaminata
Tab. 1 Risultati nel vino ad un mese dal primo trattamento
Barrique 1
trattata
Barrique 2
non trattata
Barrique 3
testimone
Carica di
Brettanomyces
(cell/ml)
40 1010 0
Barrique piuttosto contaminata
Tab. 3 Risultati del secondo trattamento
Posizione
prelievo
Barrique 1
prima
trattamento
(UFC/dm2)
Barrique 2
dopo
trattamento
(UFC/dm2)
Barrique 4
prima
trattamento
(UFC/dm2)
Barrique 4
dopo
trattamento
(UFC/dm2)
A 78000 850 8200 60
B 18000 380 3400 10
C 680000 10300 12000 420
Barrique mediamente contaminata
MANUELA CEROSIMO
VINCENZO DEL PRETE
ADOLFO PAGLIARA
EMILIA GARCIA MORUNO
C.R.A – Istituto sperimentale per
l’Enologia - Asti
9. 8 Disinfection bottling machine
As far as the treatment of bottling machines
is concerned , many tests have been done
comparing the peroxyacetic acid to the
ozonated water, these tests have
demonstrated that there is a difference of a
decimal reduction , average of 2 orders of
greatness in favor of the treatment with
ozone , it also determines in many cases a
residual charge of zero.
The percentage of elimination of the
bacterial charge was equal to 99,9999%
passing from a value of 1.435 UFC/ml of a
sample not treated, to a value of 0,0012
UFC/ml for the sample treated with ozone.
14. Exposing with ozone; LS Means
Current effect: F(3, 68)=19,857, p=,00000
Effective hypothesis decomposition
Vertical bars denote 0,95 confidence intervals
A
B
B
C
no ozone For 1 hour For 2 hour For 3 hour
Exposing with ozone
0
5
10
15
20
25
30
35
40
45
CFUFungi
Ozone can be used to treat air and so to reduce the presence of
fungus in the air , so the spores , which deposit on the product
are only a few and so the product delivered to the
customers ,develops less mustiness
Problem: abatement fungus/bacteria in the air13
15. Environmental fungus growth
in the plates with the substrate
selective fungus 1 h after
treatment with ozone
Environmental bacteria growth in
the plates with the substrate
selective bacteria after 1 hour of
treatment with ozone
Air treatment with Ozone14
16. Ozone in the Cip and to disinfect bottles15
CONCLUSIONS
The use of a CIP system with ozonated
water was more efficient for microbial load
reduction than the use of peracetic acid
alone or the combined use of soda and
peracetic acid. Sanitation with ozonated
water also tends to be much faster
because contact time is reduced and
washing is not necessary.