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Biotech Tutorial
4-Biotechnology in the
Philippines
Evelyn Mae Tecson-Mendoza, PhD
Member, National Academy of Science and Technology Philippines
Professor Emeritus, Institute of Plant Breeding, Crop Science Cluster,
College of Agriculture, University of the Philippines Los Baños
©Copyright EMTecsonMendoza 2004-2013
Policies & MilestonesPolicies & Milestones
1979 National Institutes of
Biotechnology and Applied
Microbiology established
by Presidential Decree
under Pres. Ferdinand E.
Marcos.Marcos.
Under the University of
the Philippines Los Baños,
with then Chancellor Emil
Q. Javier as concurrent
first Director.
Institute of Plant Breeding (IPB),Institute of Plant Breeding (IPB),
Crop Science Institute
College of Agriculture
University of the Philippines
Los Baños
IPB is a national institute mandated to improve and develop new varieties of crops and
conduct needed researches to support breeding objectives; lead institution for plant
biotechnology as per Seed Development Act of 1992
Biotechnology in the PhilippinesBiotechnology in the Philippines
1979 National Institute of Biotechnology and
Applied Microbiology established by PD
under Pres. FE Marcos
1986-1992 Biotechnology—flagship
program under Pres. CC Aquino
1990 EO 430 creating National Committee
on Biosafety of the Philippines (NCBP)
1992-1998 Biotechnology---major S & T
program under Pres. FV Ramos; UP Biotech
institutes established by UP Pres. EQ Javier
Biotechnology in the PhilippinesBiotechnology in the Philippines
1997 Agriculture and Fisheries Modernization
Act included provision for biotechnology
2000 Institutionalization of biotechnology in
government programs, Pres. JE Estrada
2001 Policy statement on modern biotechnology2001 Policy statement on modern biotechnology
by Pres. GM Arroyo
2002 Administrative Order (AO) No. 8 Rules and
Regulations for the Importation and Release into
the Environment of Plants and Plant Products
Derived from the Use of Modern Biotechnology
Pres. G M Arroyo’s Policy
Statement on Biotechnology
“We shall promote the safe and
responsible use of modern
biotechnology and its products as one ofbiotechnology and its products as one of
several means to achieve and sustain
food security, equitable access to health
services, sustainable and safe
environment and industry
development…”
July 2001
Agriculture
• Improved crop varieties, mostly by
conventional breeding; GM crops for
selected traits are emerging technologies
• Tissue culture raised planting materials and
Biotechnology in the
Philippines
• Tissue culture raised planting materials and
cut flowers
• Biopesticides, local formulations of Bt,
Biocon
• Biofertilizers-- Nitroplus, legume inoculant;
Azospirillum; Mycorrhiza, Nitroplus, Cocogroe, etc
• Animal improvement, embryo transfer (ET)
Biotechnology in the
Philippines
Animal health
• Vaccines, a few developed locally; mostly
imported
• Diagnostics, mostly imported• Diagnostics, mostly imported
Foods and Feeds
• Diagnostics for important pathogens
Industrial and other biotech products
• Industrial enzymes, potential technologies
• Amino acid production, mostly imported
Biotechnology in the
Philippines
• Amino acid production, mostly imported
• Specialty biomolecular chemicals,
monoglycerides, nata de coco--- need
product development
• Recovery of value-added products from
wastes, mostly imported; local potential
technologies
orchids
Prof. Emerita de
Guzman
Prof. Helen Valmayor
makapuno
orchids
1960s
1980s Dr. RC Barba led
research on banana
tissue culture at IPB
Plant Biotechnology-Based
Bioindustries in the Philippines
Tools
Equipment*
Computer
softwares Biochemicals
Process/Techniques*
Various DNA analyses*; Tissue
Institute of Plant Breeding, CA, UPLB
1975
Services
Products
Various DNA analyses*; Tissue
culture (disease-free mother plants,
for initial seedlings)**
Diagnostics*
Tissue cultured seedlings***
Transgenic crops
Genes
Pharmaceuticals*indicates relative
usage
Commercialization of Plant
Tissue Culture Technologies
Orchids
Banana
Davao Musatech (Stanfilco), largest in the
world; produces 23 M seedlings per yearworld; produces 23 M seedlings per year
Dolefil has capacity to produce 6 M per
year
Lapanday
Secura Plant Genetics
Makapuno
Sugarcane
Development of transgenic crops with
improved traits difficult to attain using
traditional breeding techniques is ongoing at
UPLB and at PhilRice.
The research in the International Rice Research Institute
based in Los Baños, Laguna is not described herein.
We are testing our transgenic papayas with
delayed ripening trait at IPB
AGSajise
ACIAR-DOST-IPB-UPLB
At
Field testing of bacterial blight resistant
transgenic rice (IR 72-Xa21
•Cloned the coat protein gene of the rice
tungro spherical virus (RSTV) and
transformed IR 72; tested transgenic rice
in screenhouse http://www.philrice.gov.ph
Philippine Rice Research Institute (Philrice) Is FieldPhilippine Rice Research Institute (Philrice) Is Field
Testing Golden RiceWith IRRI.Testing Golden RiceWith IRRI.
One of the first commercial Bt corn fields in South
Cotabato (July 2003). The lady farmer-owner said
that she would plant the Bt corn hybrid again
because of the higher yield and corn borer-
resistance. The latter meant non or less use of
pesticides.
Foreign biotech products testedForeign biotech products tested
and adopted in the Philippinesand adopted in the Philippines
For propagation
Single transformation events
◦ MON 810
◦ Corn NK603
◦ Corn GA21
◦ Corn Bt11
For direct use as food, feed or for
processing
As of 2013
Single traits/events
corn 16 potato 2
Soybean 7 sugarbeet 1
More than 700,000 ha of GM corn planted in PHL
◦ Corn Bt11
◦ MON 89034
Stacked traits
◦ Corn MON810xNK603
◦ Bt11xGA21
Canola 1 alfalfa 1
Cotton 3 rice 1
Stacked traits
corn 26
cotton 3
genomicsgenomics
study of genomics provides
knowledge and
information
On genome composition, gene expression,On genome composition, gene expression,
and metabolic processes.
Can accelerate improvement of breeds of
crops and animals through better
fundamental insights and strategies using
genomics tools
Philippine Genome ProgramPhilippine Genome Program
UP Diliman
◦ NIMBB
UP Manila
◦ IMBB◦ IMBB
◦ National Institutes of Health (NIH) UP Manila
Genome sequence & diagnostics for TB, malaria
UP Los Baños
◦ IBS, CAS
◦ IPB-CSC, CA
Agriculture Program of theAgriculture Program of the
Philippine Genome CenterPhilippine Genome Center
AIM: ENHANCE AGRICULTURALAIM: ENHANCE AGRICULTURAL
PRODUCTIVITYTHROUGH GENOMICS
LEAD AGENCY: UPLB
Drs. RP Laude, EMTecson-Mendoza,AC Laurena,
MS Mendioro, ETM Ocampo, MGQ Diaz, RN
Garcia.
Program Title: Marker Assisted Breeding
for High Fiber Quality and Virus Disease
Resistant Cultivars of Abaca
Use of GenomicsTools inUse of GenomicsTools in
Crop ImprovementCrop Improvement
Project Title: Abaca Functional
Genomics: High Throughput Discovery of
Genes and Molecular Markers
Construction and Analysis of Saba Expressed SequenceConstruction and Analysis of Saba Expressed Sequence
Tags (EST) Library and Functional Analysis of SelectedTags (EST) Library and Functional Analysis of Selected
TraitsTraits
To construct cDNA/EST libraries of Saba
To analyze the generated ESTs;To analyze the generated ESTs;
To identify novel and economically important
banana Saba genes.
L.Gueco,IPB
Field genebank of Wild Musa balbisiana collections at NPGRL
L.Gueco,IPB
Evelyn MaeEvelyn MaeTecsonTecson--Mendoza, Project LeaderMendoza, Project Leader
Study Leaders: Roberta N. Garcia, Rita P. Laude,Study Leaders: Roberta N. Garcia, Rita P. Laude, LaverneeLavernee GuecoGueco,,VisitacionVisitacion
HuelgasHuelgas
:Funded by : DA Biotech PIU:Funded by : DA Biotech PIU
Development of DNA Markers for GeneticDevelopment of DNA Markers for Genetic
Diversity Analysis, Fingerprinting and SexDiversity Analysis, Fingerprinting and Sex
Determination of Pili (Determination of Pili (Canarium ovatumCanarium ovatum))
DannyTecson2010
Roberta N. Garcia, Evelyn MaeTecson-
Mendoza and Leon O. Namuco
IPB, CA UPLB
Funded by the Department of Agriculture
BAR BIOTECH
DannyTecson2010

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Biotechnology in the Philippines - NAST

  • 1. Biotech Tutorial 4-Biotechnology in the Philippines Evelyn Mae Tecson-Mendoza, PhD Member, National Academy of Science and Technology Philippines Professor Emeritus, Institute of Plant Breeding, Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños ©Copyright EMTecsonMendoza 2004-2013
  • 2. Policies & MilestonesPolicies & Milestones 1979 National Institutes of Biotechnology and Applied Microbiology established by Presidential Decree under Pres. Ferdinand E. Marcos.Marcos. Under the University of the Philippines Los Baños, with then Chancellor Emil Q. Javier as concurrent first Director.
  • 3. Institute of Plant Breeding (IPB),Institute of Plant Breeding (IPB), Crop Science Institute College of Agriculture University of the Philippines Los Baños IPB is a national institute mandated to improve and develop new varieties of crops and conduct needed researches to support breeding objectives; lead institution for plant biotechnology as per Seed Development Act of 1992
  • 4. Biotechnology in the PhilippinesBiotechnology in the Philippines 1979 National Institute of Biotechnology and Applied Microbiology established by PD under Pres. FE Marcos 1986-1992 Biotechnology—flagship program under Pres. CC Aquino 1990 EO 430 creating National Committee on Biosafety of the Philippines (NCBP) 1992-1998 Biotechnology---major S & T program under Pres. FV Ramos; UP Biotech institutes established by UP Pres. EQ Javier
  • 5. Biotechnology in the PhilippinesBiotechnology in the Philippines 1997 Agriculture and Fisheries Modernization Act included provision for biotechnology 2000 Institutionalization of biotechnology in government programs, Pres. JE Estrada 2001 Policy statement on modern biotechnology2001 Policy statement on modern biotechnology by Pres. GM Arroyo 2002 Administrative Order (AO) No. 8 Rules and Regulations for the Importation and Release into the Environment of Plants and Plant Products Derived from the Use of Modern Biotechnology
  • 6. Pres. G M Arroyo’s Policy Statement on Biotechnology “We shall promote the safe and responsible use of modern biotechnology and its products as one ofbiotechnology and its products as one of several means to achieve and sustain food security, equitable access to health services, sustainable and safe environment and industry development…” July 2001
  • 7. Agriculture • Improved crop varieties, mostly by conventional breeding; GM crops for selected traits are emerging technologies • Tissue culture raised planting materials and Biotechnology in the Philippines • Tissue culture raised planting materials and cut flowers • Biopesticides, local formulations of Bt, Biocon • Biofertilizers-- Nitroplus, legume inoculant; Azospirillum; Mycorrhiza, Nitroplus, Cocogroe, etc • Animal improvement, embryo transfer (ET)
  • 8. Biotechnology in the Philippines Animal health • Vaccines, a few developed locally; mostly imported • Diagnostics, mostly imported• Diagnostics, mostly imported Foods and Feeds • Diagnostics for important pathogens
  • 9. Industrial and other biotech products • Industrial enzymes, potential technologies • Amino acid production, mostly imported Biotechnology in the Philippines • Amino acid production, mostly imported • Specialty biomolecular chemicals, monoglycerides, nata de coco--- need product development • Recovery of value-added products from wastes, mostly imported; local potential technologies
  • 10. orchids Prof. Emerita de Guzman Prof. Helen Valmayor makapuno orchids 1960s
  • 11. 1980s Dr. RC Barba led research on banana tissue culture at IPB
  • 12. Plant Biotechnology-Based Bioindustries in the Philippines Tools Equipment* Computer softwares Biochemicals Process/Techniques* Various DNA analyses*; Tissue Institute of Plant Breeding, CA, UPLB 1975 Services Products Various DNA analyses*; Tissue culture (disease-free mother plants, for initial seedlings)** Diagnostics* Tissue cultured seedlings*** Transgenic crops Genes Pharmaceuticals*indicates relative usage
  • 13. Commercialization of Plant Tissue Culture Technologies Orchids Banana Davao Musatech (Stanfilco), largest in the world; produces 23 M seedlings per yearworld; produces 23 M seedlings per year Dolefil has capacity to produce 6 M per year Lapanday Secura Plant Genetics Makapuno Sugarcane
  • 14. Development of transgenic crops with improved traits difficult to attain using traditional breeding techniques is ongoing at UPLB and at PhilRice. The research in the International Rice Research Institute based in Los Baños, Laguna is not described herein.
  • 15. We are testing our transgenic papayas with delayed ripening trait at IPB AGSajise ACIAR-DOST-IPB-UPLB
  • 16. At Field testing of bacterial blight resistant transgenic rice (IR 72-Xa21 •Cloned the coat protein gene of the rice tungro spherical virus (RSTV) and transformed IR 72; tested transgenic rice in screenhouse http://www.philrice.gov.ph
  • 17. Philippine Rice Research Institute (Philrice) Is FieldPhilippine Rice Research Institute (Philrice) Is Field Testing Golden RiceWith IRRI.Testing Golden RiceWith IRRI.
  • 18. One of the first commercial Bt corn fields in South Cotabato (July 2003). The lady farmer-owner said that she would plant the Bt corn hybrid again because of the higher yield and corn borer- resistance. The latter meant non or less use of pesticides.
  • 19. Foreign biotech products testedForeign biotech products tested and adopted in the Philippinesand adopted in the Philippines For propagation Single transformation events ◦ MON 810 ◦ Corn NK603 ◦ Corn GA21 ◦ Corn Bt11 For direct use as food, feed or for processing As of 2013 Single traits/events corn 16 potato 2 Soybean 7 sugarbeet 1 More than 700,000 ha of GM corn planted in PHL ◦ Corn Bt11 ◦ MON 89034 Stacked traits ◦ Corn MON810xNK603 ◦ Bt11xGA21 Canola 1 alfalfa 1 Cotton 3 rice 1 Stacked traits corn 26 cotton 3
  • 20. genomicsgenomics study of genomics provides knowledge and information On genome composition, gene expression,On genome composition, gene expression, and metabolic processes. Can accelerate improvement of breeds of crops and animals through better fundamental insights and strategies using genomics tools
  • 21. Philippine Genome ProgramPhilippine Genome Program UP Diliman ◦ NIMBB UP Manila ◦ IMBB◦ IMBB ◦ National Institutes of Health (NIH) UP Manila Genome sequence & diagnostics for TB, malaria UP Los Baños ◦ IBS, CAS ◦ IPB-CSC, CA
  • 22. Agriculture Program of theAgriculture Program of the Philippine Genome CenterPhilippine Genome Center AIM: ENHANCE AGRICULTURALAIM: ENHANCE AGRICULTURAL PRODUCTIVITYTHROUGH GENOMICS LEAD AGENCY: UPLB Drs. RP Laude, EMTecson-Mendoza,AC Laurena, MS Mendioro, ETM Ocampo, MGQ Diaz, RN Garcia.
  • 23. Program Title: Marker Assisted Breeding for High Fiber Quality and Virus Disease Resistant Cultivars of Abaca Use of GenomicsTools inUse of GenomicsTools in Crop ImprovementCrop Improvement Project Title: Abaca Functional Genomics: High Throughput Discovery of Genes and Molecular Markers
  • 24. Construction and Analysis of Saba Expressed SequenceConstruction and Analysis of Saba Expressed Sequence Tags (EST) Library and Functional Analysis of SelectedTags (EST) Library and Functional Analysis of Selected TraitsTraits To construct cDNA/EST libraries of Saba To analyze the generated ESTs;To analyze the generated ESTs; To identify novel and economically important banana Saba genes.
  • 25. L.Gueco,IPB Field genebank of Wild Musa balbisiana collections at NPGRL L.Gueco,IPB Evelyn MaeEvelyn MaeTecsonTecson--Mendoza, Project LeaderMendoza, Project Leader Study Leaders: Roberta N. Garcia, Rita P. Laude,Study Leaders: Roberta N. Garcia, Rita P. Laude, LaverneeLavernee GuecoGueco,,VisitacionVisitacion HuelgasHuelgas :Funded by : DA Biotech PIU:Funded by : DA Biotech PIU
  • 26. Development of DNA Markers for GeneticDevelopment of DNA Markers for Genetic Diversity Analysis, Fingerprinting and SexDiversity Analysis, Fingerprinting and Sex Determination of Pili (Determination of Pili (Canarium ovatumCanarium ovatum)) DannyTecson2010 Roberta N. Garcia, Evelyn MaeTecson- Mendoza and Leon O. Namuco IPB, CA UPLB Funded by the Department of Agriculture BAR BIOTECH DannyTecson2010