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Stem node of Miniature Rose with axillary bud were used as explants. These explants cultured on MS medium supplemented with different concentrations of 2,4-D. It was found that MS medium supplemented with 0.5 mg/l 2,4-D gave the highest number of green callus. The callus cultured on MS medium supplemented with different combinations of NAA and BA to form new shoot and root. From the result, we are able to find the highest number of young shoots that were induced from callus when cultured callus on MS medium supplemented with NAA and BA. When subcultured all new shoots with the same size to MS medium supplemented with different concentrations of NAA and BA, and 2,4- D for six weeks. The result was significant difference (P≤0.5) when compared the average height of plant and percentage of root formation, but their duration time for flowering were not significant different.