1.
1 2 3 4 1
MIX
BIOGLYPHICS
VISUAL LAB PROTOCOLS. CREATED USING A SIMPLE DRAG & DROP WEB APP.
100% OPEN SOURCE. COMPATIBLE WITH LAB AUTOMATION SOFTWARE.
42 C 0 C
37ºC
A 2014 GENSPACE IGEM PROJECT

2.
2
Right
now
lab
protocols
are
usually
wri4en
in
text.
Example:

3.
3
There
are
4
problems
with
text
protocols.
1.
Poor
Consistency
Each
person
wri0ng
a
protocol
phrases
things
a
bit
differently
and
focuses
on
different
content.
For
a
beginner
in
the
lab
this
can
be
confusing.
“Spin
down”
vs.
“Centrifuge”
2.
Poor
VisualizaBon
It
can
be
hard
for
a
beginner
in
a
lab
to
connect
the
text
of
a
protocol
with
clear
ac0ons
they
need
to
perform.
3.
Low
Efficiency
Wri0ng
text
protocols
is
not
very
0me
efficient.
You
need
to
spend
0me
deciding
word
choice
and
what
level
of
detail
to
cover.
4.
Machine
Illegible
Because
the
text
of
protocols
is
not
consistent
in
language
or
formaHng,
computer
programs
that
run
lab
automa0on
soJware
are
not
able
to
use
it.

4.
4
What
if...
Instead
of
this. You
had
this.

5.
5
MAKING
THE
DREAM
REAL:
WE
GOT
THE
COMMUNITY
INVOLVED
On
7/7/14
we
hosted
a
public
event
-­‐
The
BioGlyphics
Iconathon.
We
brought
together
graphic
designers
and
ar0sts
from
all
over
the
NYC
metro
area
and
invited
them
to
design
BIOGLYPHICS
at
a
event
hosted
in
a
Brooklyn
community
work
space.

6.
6
BIOGLYPHICS: A GENSPACE PROJECT 7/3/14
IGEM TRANSFORMATION PROTOCOL
An
Early
PURPOSE
Prototype MATERIALS
This procedure allows you to insert a BioBrick part (from the IGEM distribution kit) into cells. The IGEM distrubution kit contains an array of
wells that contain plasmids. These plasmids contain the DNA for the BioBrick part. This plasmid will be inserted into the cell and then the cell
will be plated on a petri dish.
1
2
B
C
3
W
A
competent
cells
IGEM
distribution
w/ DNA
petri dish
w/ LB agar
& appropriate
antibiotic
2 ml tube
SOC
media
deionized
water
RFP
control
50μL 2μL MIX 2μL 2μL
1 A W A 2 A
42 C
1 2 3 4 1
MIX
B B B
30 MIN 1 MIN
0 C
200μL
A A 3
A
5 MIN
0 C
A
2 HOURS
37ºC
SHAKE OR TUMBLE
A
200μL
A C
SPREAD
C
13 HOURS
AGAR SIDE UP
37ºC
C
PROCEDURE
1 2 3 4 5
6 7 8 9 10
11 12 13
The
complete
IGEM
transforma0on
protocol
in
BIOGLYPHICS.

7.
50μL 2μL MIX 2μL 2μL
1 A W A 2 A
2 HOURS
7
Why
We
Like
It.
100%
consistent.
Each
protocol
follows
the
same
logical
format.
Easy
to
understand
and
visualize.
Materials
and
Procedures
are
visually
defined
just
like
IKEA
instruc0ons.
Fast
&
easy
to
create.
Simple
drag
and
drop
web/
android/iOS
app
interface.
Will
be
100%
machine
readable
for
lab
automaBon
so^ware.
Making
a
protocol
in
this
format
will
automa0cally
build
machine
code
behind
the
scenes.
MATERIALS
1
2
B
SOC
media
A competent
cells
C
3
W
IGEM
distribution
w/ DNA
petri dish
w/ LB agar
& appropriate
antibiotic
2 ml tube
deionized
water
RFP
control
42 C
1 2 3 4 1
MIX
B B B
30 MIN 1 MIN
0 C
200μL
A A 3
A
5 MIN
0 C
A
37ºC
SHAKE OR TUMBLE
A
200μL
A C
SPREAD
C
13 HOURS
AGAR SIDE UP
37ºC
C
PROCEDURE
1 2 3 4 5
6 7 8 9 10
11 12 13

8.
8
Our
Dream
is...
To
create
a
fully
featured
online
community
and
repository
of
visual
laboratory
protocols.
Imagine
a
Thingiverse.com
or
allrecipies.com
for
visual
laboratory
protocols.

9.
Drag
and
drop
web
app
for
creaBng
a
BIOGLYPHICS
protocol.
Simply
drag
and
drop
the
correct
icons
and
then
fill
in
the
details.
In
under
2
mins
you
can
build
a
complete
protocol.

10.
10
It
works
just
like
IKEA
InstrucBons
STEP
1:
DEFINE
YOUR
MATERIALS
We
have
a
test
tube
of
Soc
Media
defined
as
1
STEP
2:
CREATE
YOUR
PROCEDURE
Use
procedure
icons
and
materials
to
create
your
protocol.
We
have
an
empty
2ml
Tube
defined
as
A
We
are
pipeHng
5
microliters
of
1
into
A.
We
centrifuge
A
for
30
sec.
We
incubate
&
shake
A
for
24
hours.

11.
11
When
you
create
a
visual
protocol...
The
web
app
will
automaBcally
generate
lab
automaBon
code.
The
code
you
see
here
is
an
early
a4empt
at
translaBng
Bioglyphics
input
into
Pr-­‐Pr

12.
BIOGLYPHICS:
A 3 STAGE PROCESS OF DEVELOPMENT
12
STAGE
1
Proof
of
concept
and
basic
early
prototype.
Ini0al
development
of
Bioglyphic
icons
and
explora0on
into
lab
automa0on
programming.
STAGE
2
Raise
funding
and
find
dedicated
volunteers
for
the
full
development
of
the
web
applica0on
and
online
community.
STAGE
3
Spread
BioGlyphics
to
other
community
labs,
teachers,
and
bio
makers
across
the
country.
WE
ARE
HERE 2015-­‐2016 Late
2016