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IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 252
INVESTIGATION OF MECHANICAL PROPERTIES ON VINYLESTER
BASED BIO-COMPOSITE WITH GELATIN AS RANDOMLY
DISTRIBUTED FILLER MATERIAL
Avinash S1
, H.G.Hanumantharaju2
, Vignesh M3
, Akash S4
1
Student, dept. of Mechanical Engineering, University Visvesvaraya College of Engineering, Bangalore,
Karnataka, India
2
Faculty, dept. of Mechanical Engineering, University Visvesvaraya College of Engineering, Bangalore,
Karnataka, India
3
Student, dept. of Mechanical Engineering, University Visvesvaraya College of Engineering, Bangalore,
Karnataka, India
4
Student, dept. of Mechanical Engineering, Sir MVIT, Bangalore, Karnataka, India
Abstract
In the present work an attempt has been made to develop Bio-composite Material using raw dupion silk fiber reinforced vinylester
matrix in addition with gelatin as filler material with varying percentage using hand lay-up method. The experiments namely
tensile, flexural and hardness test were conducted on the standard samples prepared. An appreciable improvement was found in the
mechanical properties of the Bio-composite, which can be used for variety of applications in the human bone replacement.
The biocomposites were prepared in varying percentage of filler addition (0%, 10%, 20%, and 30%) and different mechanical tests
(tensile, flexure and hardness) were conducted on the samples prepared as per ASTM standards. According to literature survey the
human femur bone with tensile strength for female is 30.08±7.96MPa. In this work it was found that tensile strength of 30% gelatin
filled with Dupion silk fiber reinforced vinylester bio-composite material is 26.86 MPa.
Keywords: Implantation, Bio-composite, Dupion Silk Fiber, Gelatin.
--------------------------------------------------------------------***-------------------------------------------------------------------
1. INTRODUCTION
Engineering Materials are used in medical application to
make devices to replace a part or a function of the body in
safe, reliably economically, and physiologically
acceptable manner. A variety of devices and materials
are used in the treatment of disease or injury.
Common place examples include suture needles, plates,
teeth fillings, etc
Large numbers of polymeric materials alone and in
combination with other materials are becoming
increasingly significant in the field of biomaterials, as a
result composite materials becomes an optimum choice
for such applications.
The use of petroleum based products as constituents in
polymer matrix composite has raised concerns regarding
environmental issue and non-renewability of the resource.
Hence the problems associated with it have initiated the
efforts to develop Biocomposites. The development of
biocomposites has attracted great interests among
researchers worldwide. This is because, biocomposite
have one or more constituents that are obtained from
natural renewable resources also they may have partial or
complete degradation and they do not emit any toxic
substance during production and disposal process. By
successfully producing biocomposite, we may be able to
substitute the conventional petroleum-based plastics in
various applications.
Bio-Composite are used in biomedical applications for
drug/gene delivery and tissue engineering applications.
They often mimic the structures of the living materials
involved in the process in addition to the strengthening
properties of the matrix that was used but still providing
biocompatibility, e.g. in creating scaffolds in bone tissue
engineering.
Metals and bioceramics for biomedical application have
yielded limited successes yet substantial mismatch
between their properties and bone tissue persist, thereby
punctuating the need for tissue engineered products.
However, metals commonly induce stress shielding and
will eventually experience wear debris, ultimately leading
to implant failure [1]. For example, a fractured bone,
fixated with a rigid, non-biodegradable stainless steel
implant, has a tendency for re-fracture upon removal of
the implant. The bone does not carry sufficient load during
the healing process, because the load is carried by the
rigid stainless steel [2].
However an implant prepared from biodegradable
polymer can be engineered to degrade at a rate that will
IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 253
slowly transfer load to the healing bone [3] as shown in
Graph-1. Materials used to achieve bone regeneration are
diverse but not limited to metals, ceramics, synthetic
polymers, naturally derived polymers, and other
biocompatible substances. Success has been found by
combining these materials as a strategy to eliminate the
disadvantages of an individual material.
In the case of materials where starch is used as an additive
to a conventional plastic matrix, the polymer in contact
with the soil and/or water is attacked by the microbes. The
microbes digest the starch, leaving behind a porous,
sponge like structure with a high interfacial area, and low
structural strength. When the starch component has been
depleted, the polymer matrix begins to be degraded by an
enzymatic attack. Each reaction results in the scission of a
molecule, slowly reducing the weight of the matrix until
the entire material has been digested [4].
Graph- 1: Mechanical Strength vs Time for Bone and
Plate
The current work is an effort to utilize the natural
renewable resources to develop biocomposite material for
biomedical applications such as bone Plates, bone screws
material for both internal, external fixations. The dupion
silk fiber is a natural fiber, it is used as reinforcement in
vinyl ester resin matrix and gelatin is used as fillers.
2. PREPARATION OF BIOCOMPOSITES
2.1 Materials
Unsaturated vinylester resin was selected as matrix
material, Dupion silk fibers as reinforcement and gelatin
as filler material.
Vinyl ester resins having density 1.05 g/cm3
are becoming
increasingly important for fiber reinforced composites.
They combine the excellent mechanical, chemical and
solvent resistance properties of epoxy resins with the
properties found in the unsaturated polyester resins. The
chemical structure of vinylester resin is shown in Fig.1
Fig 1: chemical structure of a typical vinylester resin
Silk Dupioni is a type of silk fiber created with the threads
from two different silk worms. When two worms spin
their cocoons close together, the fibers get tangled up;
these naturally tangled fibers are then used together to
make the silk thread. In spite of its delicate appearance,
silk is relatively robust; the thread is rougher than regular
silk as shown in Fig.2.
Gelatin is a protein obtained by boiling skin, tendons,
ligaments, and/or bones with water. It is usually obtained
from cows or pigs. Gelatin is used in shampoos, face
masks, and other cosmetics; as a thickener for fruit
gelatins and puddings (such as Jell-O); in candies,
marshmallows, cakes, ice cream, and yogurts; on
photographic film; and in vitamins as a coating and as
capsules, and it is sometimes used to assist in “clearing”
wines.
They have good Biocompatibility, Biodegradable and its
degradation products are non-toxic.
Table-1: Material Procurement Table
Sl.
No.
MATERIALS SUPPLIERS
1
Unsaturated Vinylester
Resin
Naphtha Resins &
Chemicals (p),
Bangalore, India
2
Cobalt napthalate
accelerator
3
Methyl Ethyl Ketone
peroxide catalyst
4 Dupion Silk Fiber
Silk Exchange Office,
Bangalore, India
5 Edible Gelatin
Food world, Bangalore,
India
2.2 Technique
The laminates were prepared by the hand lay-up technique
on the rectangular box. The rectangular box of dimension
19cm×12cm is cleaned with soft brush. A layer of wax is
coated on the cleaned surface for the easy removal of the
laminate after curing.
Weighed quantity of Gelatin (fillers), Methyl ethyl ketone
peroxide (catalyst), Cobalt napthalate (accelerator) are
added to Vinyl ester resin taken in the bowl and stirred
well for uniform distribution. The weight of constituents
to be taken for each specimen is shown in Table-2 and
Table-3. The catalyst and accelerator is taken at 1ml each
for the 100 g of vinyl ester resin taken.
The first layer of Vinyl ester resin mixture is coated on the
wax, later Dupion silk fibers are placed in the mould and
the resin mixture is poured and distributed uniformly.
Same procedure is repeated until the desired thickness is
obtained. Alternate layers of resin and Dupion silk fiber
are placed.
IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 254
Fig 2: Raw dupion silk fibers
The laminate is cured under light pressure for 2 hrs,
followed by curing at room temperature for 24 hours. By
following the same procedure as said above bio-composite
material having filler composition of 0%, 10%, 20% and
30% is prepared. Fig.3 shows the final laminate.
Fig-3: Prepared biocomposite laminate
The prepared Bio-Composite materials are cut into
standard ASTM Dimensions using abrasive water jet
machine.
2.3 Sample Preparation Calculation
 Density of vinyl ester resin = 1.05 g/cm3
 Density of Gelatin = 0.98 g/cm3
 Density of silk fiber = 1.33 g/cm3
 Volume of the mold, V = 190 x 120 x 3.5 = 79800
mm3
= 79.8 cm3
= 80 cm3
Table-2: Composition of Biocomposite
Samples
% of
Resin
% of
Fiber
% of
Fillers
A 90 10 0
B 80 10 10
C 70 10 20
D 60 10 30
Table -3: Density Calculation of Samples
Mass of
Resin
(grams)
Mass of
Fiber
(grams)
Mass of
fillers
(grams)
Total
mass
(grams)
Density
(g/cm3
)
75.6 10.64 0 86.24 1.078
67.2 10.64 7.84 85.68 1.071
58.8 10.64 15.68 85.12 1.064
50.4 10.64 23.52 84.56 1.057
3. EXPERIMENTATION
Mechanical tests such as tensile test, flexural test and
hardness tests were conducted on the specimens prepared
as per the ASTM standards. The tensile test and flexural
test was conducted using J J Lloyd universal testing
machine and the hardness test was conducted using
Rockwell hardness testing machine. All the tests were
carried out at room temperature.
Fig-4: J J Lloyd UTM
3.1 Tensile Test
Tensile tests on composite specimens were carried out
according to ASTM–D 638 standard to determine tensile
strength and to observe the behavior of biocomposites
under load.
Fig -5: Standard tensile specimen dimensions
3.2 Flexural Test
Flexural tests on composite specimens were carried out
according to ASTM D-790 standard.
Fig -6: Standard flexural specimen dimensions
3.3 Rockwell Hardness Test
The Rockwell hardness is determined by the depth of the
indentation in the test material resulting from application
of a given force on a specific indenter.
4. RESULT AND DISCUSSION
In this section the results obtained from tension, Flexural
and Hardness tests are tabulated and represented
graphically to analyze the behavior of Biocomposites.
IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 255
4.1 Tensile Test
The peak load and tensile strength obtained from tensile
test conducted on the prepared specimens is tabulated in
Table -4.
Table 4: Tensile Strength readings of each trail
SAMPLE TRIALS
PEAK
LOAD
(N)
TENSILE
STRENGTH
(N/mm2)
A
313 13.55 13.55
432 17.36 17.36
B
599 23.21 33.72
291 11.82 37.70
C
771 26.24 37.70
621 20.71 28.60
D
892 27.05 26.12
976 26.68 18.33
The prepared tensile test specimens before and after the
tensile test experiment is shown in the figure-7
4.1.1 Effect of Peak Load
From Graph 2 it can be observed that at all filler content
the highest Peak Load is for 30% filler content bio-
composite with a value of 934 N.
Graph-2: Peak load vs Gelatin filler
4.1.2 Effect of Ultimate Tensile Strength
From Graph-3 it can be observed that at all filler content
the highest Ultimate Tensile Strength is for 30% filler
content bio-composite with a value of 26.86Mpa.
Graph-3: Tensile strength vs Gelatin filler
4.2 Flexural Test
The peak load and compression strength obtained from
compression test conducted on the prepared specimens is
tabulated in table-5.
Table -5: Flexural Strength readings of each trail
SAMPLE TRIALS
PEAK
LOAD (N)
FLEXURAL
STRENGTH
(N/mm2
)
A
TRIAL 1 222 37.82
TRIAL 2 217 35.13
B
TRIAL 1 118 23.33
TRIAL 2 102 19.77
C
TRIAL 1 107 17.39
TRIAL 2 69 11.26
D
TRIAL 1 31 6.13
TRIAL 2 71 12.67
The prepared flexural test specimens before and after the
flexural test experiment is shown in the figure-8
Fig-8: Flexural Specimen Before and After Test
IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 256
4.2.1 Effect of Peak Load
From Graph 4 it can be observed that at all filler content
the highest Peak load is for 0% filler content bio-
composite with a value of 219.5N
Graph-4: Peak load vs Gelatin filler
4.2.2 Effect of Flexural Strength
From Graph-5 it can be observed that at all filler content
the highest Flexural Strength is for 0% filler content bio-
composite with a value of 36.47Mpa.
Graph-5: Flexural Strength vs Gelatin Filler
4.3 Hardness Test
Hardness is a surface property and is a measure of wear
resistance on the surface of the composite. Hardness value
increases from 0% filler content to 30%.The measured
hardness number test results are shown in Table 6.
Graph-6: HRBN vs Gelatin filler
Table-6: Measured Hardness Number
SPECIMEN COMPOSITION HRBN
A
Vinylester resin + Dupion
silk fiber
15
B
Vinylester resin + Dupion
silk fiber + 10% Gelatin
20
C
Vinylester resin + Dupion
silk fiber + 20% Gelatin
24
D
Vinylester resin + Dupion
silk fiber + 30% Gelatin
28
4.4 Morphological Characteristics
Below figures show the micrograph of sample revealing
the morphology of Gelatin Granules and silk fiber
reinforcement.
Fig-9: Gelatin Granules & Silk fibers at 500 magnification
Fig-10: SEM micrograph of untreated Dupion silk fiber
The Micrographs reveal that the bonding between
reinforcement and matrix is poor and the Gelatin granules
are attracted towards the fibers since both are hydrophilic
in nature.
IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 257
5. CONCLUSION
 The present work has been undertaken, with an
objective to explore the potential of the natural fiber
polymer Bio-composites and to study the mechanical
properties of the bio-composites.
 Bio-composites can supplement and eventually
replace petroleum based composite materials in
several applications thus offering new agricultural,
environmental, manufacturing and consumer
benefits.
 In mechanical test, it was observed that 30% filler
content has better tensile properties compared to the
0%,10%,20% filler content, the flexural strength is
highest for 0% filler content, and it reduces for
10%,20%,30%.
 The hardness test results shows that 30% filler
content Biocomposites has better Hardness number.
 In this work Tensile strength of 30% Gelatin filled
Natural (Dupion silk) fiber reinforcement vinylester
composite material matches the femur bone tensile
strength.
REFERENCES
[1] H.E. Davis and J.K. Leach, Hybrid and
Composite Biomaterials in Tissue Engineering, Topics in
Multifunctional Biomaterials and Devices, Ed. N
Ashammakhi © 2008.
[2] Sangamesh Kumbar, Cato Laurencin, Meng
Deng, natural and synthetic biomedical polymers (Elsevier
Inc.)
[3] John C. Middleton, Arthur J. Tipton, Synthetic
biodegradable polymers as orthopedic devices,
Biomaterials 21 (2000) 2335-2346
[4] A. Ashwin Kumar , Karthick. K, and K. P.
Arumugam, Biodegradable Polymers and Its Applications,
International Journal of Bioscience, Biochemistry and
Bioinformatics, Vol. 1, No. 3, September 2011
[5] Dr.Shivappa, Ananda.G.K, Shivakumar.N,
“Mechanical Characterization of Rice Husk Flour
Reinforced Vinylester Polymer Composite", International
Journal of Innovative Research in Science, Engineering
and Technology, ISSN: 2319-8753, Vol. 2, Issue 11,
November 2013.
[6] M.SAKTHIVEl, S.RAMESH, "Mechanical
Properties of Natural Fibre (Banana, Coir, Sisal) Polymer
Composites", SCIENCE PARK ISSN: 2321 – 8045, Vol-
1, Issue-1, July 2013
[7] BIRSAN, I[ulian] G[abriel]; ROMAN, I[gor];
BRIA, V[asile]; UNGUREANU, V[ictor] &
CIRCIUMARU, A[drian], "STARCH - EPOXY
COMPOSITES", Annals of DAAAM for 2011 &
Proceedings of the 22nd International DAAAM
Symposium, Volume 22, No. 1, ISSN 1726-9679 ISBN
978-3-901509-83-4, 2011.
[8] Shinji Ochi, "Tensile Properties of Bamboo Fiber
Reinforced Biodegradable Plastics”, International Journal
of Composite Materials, 2012, 1-4.
[9] Munikenche Gowda T., Naidu A. C. B., and
Rajput Chayya, “Some Mechanical Properties of
Untreated Jute Fabric-Reinforced Polyester Composites”,
Journal of Composites Part A: Applied Science and
Manufacturing, vol.30, pp. 277- 284, 1999.
[10] Manikandan Nair K.C., Diwan S. M., Thomas S.,
“Tensile properties of short sisal fibre reinforced
polystyrene composites” Journal of Applied Polymer
Science, New York, vol.60, pp.1483-1497, 1996.
[11] Paramasivam T., Abdulkalam A. P. J., “On the
study of natural fibre composites”. Fibre Science and
Technology, New Delhi, vol.1, pp.85-98, 1974.
[12] Shah A. N. and Lakkad S. C., “Mechanical
Properties Of Jute-Reinforced Plastics”, Fibre Science and
Technology vol.15 pp.41-46, 1981.
[13] Pickering K.L., Li Y., Farrell R.L., & Lay M.,
“Interfacial modification of hemp fibre reinforced
composites using fungal and alkali treatment”. Biobased
Material and Bioenergy, vol.1 (1), pp.109-117, 2007.
[14] Gassan J. and Bledzki A.K., “The influence of
fiber-surface treatment on the mechanical properties of
jute-polypropylene composites”. Composites Part-A
vol.28, pp.1001-1005, 1997.
[15] K. Murali Mohan Rao, K. Mohana Rao,
"Extraction and tensile properties of natural fibers: Vakka,
date and bamboo Composite Structures" 77 (2007) 288–
295.
[16] Dr.KRDinesh1,JagadishSP, Dr. A
Thimmanagouda,Dr. Neeta Hatapaki, “Characterization
and Investigation of Tensile and Compression Test on
Sisal Fiber Reinforcement Epoxy Composite Materials
Used as Orthopaedic Implant”
BIOGRAPHIES
Mr. Avinash S received his B.E degree
in Mechanical Engineering from VTU,
India (2009), presently doing his M.E
degree in advanced materials &
technology at UVCE, Bangalore
University, India. Presently his project is
based on the field of Biomaterials,
replacement of femur bone. His research interests include
Composites, Nano-Technology and Cryogenics
Dr. H.G.Hanumantharaju presently
working as assistant professor in UVCE,
Bangalore university India. He obtained
his B.E degree in mechanical
engineering from Mysore University,
India (1991) and ME in Machine Design
from UVCE, Bangalore University
(2004). He obtained his Ph.D in the field of Bio-Materials
from Bangalore University (2012). He has guided more
than 20 ME students and currently guiding 5 research
scholars. His field of interests include bio-materials,
composites, stress analysis etc.,
Mr. Vignesh M received his B.E degree in
Mechanical Engineering from VTU
(2012), presently doing his M.E degree in
advanced materials & technology at
UVCE, Bangalore University. Presently
IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308
_______________________________________________________________________________________
Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 258
his project is based on the field of Biomaterials,
Functional Bone fixation Devices. His research interests
include Composites, Nano-Technology, Cellular Materials
and Cryogenics.
Mr. Akash.S is presently working as
ISAGO Trainee member at Menzies
aviation Bangalore, India and completed
B.E in industrial engineering and
management from Sir MVIT, Bangalore
(2014). His research interests include
Bio-Materials and industrial relation and
management.

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Investigation of mechanical properties on vinylester based bio composite with gelatin as randomly distributed filler material

  • 1. IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 252 INVESTIGATION OF MECHANICAL PROPERTIES ON VINYLESTER BASED BIO-COMPOSITE WITH GELATIN AS RANDOMLY DISTRIBUTED FILLER MATERIAL Avinash S1 , H.G.Hanumantharaju2 , Vignesh M3 , Akash S4 1 Student, dept. of Mechanical Engineering, University Visvesvaraya College of Engineering, Bangalore, Karnataka, India 2 Faculty, dept. of Mechanical Engineering, University Visvesvaraya College of Engineering, Bangalore, Karnataka, India 3 Student, dept. of Mechanical Engineering, University Visvesvaraya College of Engineering, Bangalore, Karnataka, India 4 Student, dept. of Mechanical Engineering, Sir MVIT, Bangalore, Karnataka, India Abstract In the present work an attempt has been made to develop Bio-composite Material using raw dupion silk fiber reinforced vinylester matrix in addition with gelatin as filler material with varying percentage using hand lay-up method. The experiments namely tensile, flexural and hardness test were conducted on the standard samples prepared. An appreciable improvement was found in the mechanical properties of the Bio-composite, which can be used for variety of applications in the human bone replacement. The biocomposites were prepared in varying percentage of filler addition (0%, 10%, 20%, and 30%) and different mechanical tests (tensile, flexure and hardness) were conducted on the samples prepared as per ASTM standards. According to literature survey the human femur bone with tensile strength for female is 30.08±7.96MPa. In this work it was found that tensile strength of 30% gelatin filled with Dupion silk fiber reinforced vinylester bio-composite material is 26.86 MPa. Keywords: Implantation, Bio-composite, Dupion Silk Fiber, Gelatin. --------------------------------------------------------------------***------------------------------------------------------------------- 1. INTRODUCTION Engineering Materials are used in medical application to make devices to replace a part or a function of the body in safe, reliably economically, and physiologically acceptable manner. A variety of devices and materials are used in the treatment of disease or injury. Common place examples include suture needles, plates, teeth fillings, etc Large numbers of polymeric materials alone and in combination with other materials are becoming increasingly significant in the field of biomaterials, as a result composite materials becomes an optimum choice for such applications. The use of petroleum based products as constituents in polymer matrix composite has raised concerns regarding environmental issue and non-renewability of the resource. Hence the problems associated with it have initiated the efforts to develop Biocomposites. The development of biocomposites has attracted great interests among researchers worldwide. This is because, biocomposite have one or more constituents that are obtained from natural renewable resources also they may have partial or complete degradation and they do not emit any toxic substance during production and disposal process. By successfully producing biocomposite, we may be able to substitute the conventional petroleum-based plastics in various applications. Bio-Composite are used in biomedical applications for drug/gene delivery and tissue engineering applications. They often mimic the structures of the living materials involved in the process in addition to the strengthening properties of the matrix that was used but still providing biocompatibility, e.g. in creating scaffolds in bone tissue engineering. Metals and bioceramics for biomedical application have yielded limited successes yet substantial mismatch between their properties and bone tissue persist, thereby punctuating the need for tissue engineered products. However, metals commonly induce stress shielding and will eventually experience wear debris, ultimately leading to implant failure [1]. For example, a fractured bone, fixated with a rigid, non-biodegradable stainless steel implant, has a tendency for re-fracture upon removal of the implant. The bone does not carry sufficient load during the healing process, because the load is carried by the rigid stainless steel [2]. However an implant prepared from biodegradable polymer can be engineered to degrade at a rate that will
  • 2. IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 253 slowly transfer load to the healing bone [3] as shown in Graph-1. Materials used to achieve bone regeneration are diverse but not limited to metals, ceramics, synthetic polymers, naturally derived polymers, and other biocompatible substances. Success has been found by combining these materials as a strategy to eliminate the disadvantages of an individual material. In the case of materials where starch is used as an additive to a conventional plastic matrix, the polymer in contact with the soil and/or water is attacked by the microbes. The microbes digest the starch, leaving behind a porous, sponge like structure with a high interfacial area, and low structural strength. When the starch component has been depleted, the polymer matrix begins to be degraded by an enzymatic attack. Each reaction results in the scission of a molecule, slowly reducing the weight of the matrix until the entire material has been digested [4]. Graph- 1: Mechanical Strength vs Time for Bone and Plate The current work is an effort to utilize the natural renewable resources to develop biocomposite material for biomedical applications such as bone Plates, bone screws material for both internal, external fixations. The dupion silk fiber is a natural fiber, it is used as reinforcement in vinyl ester resin matrix and gelatin is used as fillers. 2. PREPARATION OF BIOCOMPOSITES 2.1 Materials Unsaturated vinylester resin was selected as matrix material, Dupion silk fibers as reinforcement and gelatin as filler material. Vinyl ester resins having density 1.05 g/cm3 are becoming increasingly important for fiber reinforced composites. They combine the excellent mechanical, chemical and solvent resistance properties of epoxy resins with the properties found in the unsaturated polyester resins. The chemical structure of vinylester resin is shown in Fig.1 Fig 1: chemical structure of a typical vinylester resin Silk Dupioni is a type of silk fiber created with the threads from two different silk worms. When two worms spin their cocoons close together, the fibers get tangled up; these naturally tangled fibers are then used together to make the silk thread. In spite of its delicate appearance, silk is relatively robust; the thread is rougher than regular silk as shown in Fig.2. Gelatin is a protein obtained by boiling skin, tendons, ligaments, and/or bones with water. It is usually obtained from cows or pigs. Gelatin is used in shampoos, face masks, and other cosmetics; as a thickener for fruit gelatins and puddings (such as Jell-O); in candies, marshmallows, cakes, ice cream, and yogurts; on photographic film; and in vitamins as a coating and as capsules, and it is sometimes used to assist in “clearing” wines. They have good Biocompatibility, Biodegradable and its degradation products are non-toxic. Table-1: Material Procurement Table Sl. No. MATERIALS SUPPLIERS 1 Unsaturated Vinylester Resin Naphtha Resins & Chemicals (p), Bangalore, India 2 Cobalt napthalate accelerator 3 Methyl Ethyl Ketone peroxide catalyst 4 Dupion Silk Fiber Silk Exchange Office, Bangalore, India 5 Edible Gelatin Food world, Bangalore, India 2.2 Technique The laminates were prepared by the hand lay-up technique on the rectangular box. The rectangular box of dimension 19cm×12cm is cleaned with soft brush. A layer of wax is coated on the cleaned surface for the easy removal of the laminate after curing. Weighed quantity of Gelatin (fillers), Methyl ethyl ketone peroxide (catalyst), Cobalt napthalate (accelerator) are added to Vinyl ester resin taken in the bowl and stirred well for uniform distribution. The weight of constituents to be taken for each specimen is shown in Table-2 and Table-3. The catalyst and accelerator is taken at 1ml each for the 100 g of vinyl ester resin taken. The first layer of Vinyl ester resin mixture is coated on the wax, later Dupion silk fibers are placed in the mould and the resin mixture is poured and distributed uniformly. Same procedure is repeated until the desired thickness is obtained. Alternate layers of resin and Dupion silk fiber are placed.
  • 3. IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 254 Fig 2: Raw dupion silk fibers The laminate is cured under light pressure for 2 hrs, followed by curing at room temperature for 24 hours. By following the same procedure as said above bio-composite material having filler composition of 0%, 10%, 20% and 30% is prepared. Fig.3 shows the final laminate. Fig-3: Prepared biocomposite laminate The prepared Bio-Composite materials are cut into standard ASTM Dimensions using abrasive water jet machine. 2.3 Sample Preparation Calculation  Density of vinyl ester resin = 1.05 g/cm3  Density of Gelatin = 0.98 g/cm3  Density of silk fiber = 1.33 g/cm3  Volume of the mold, V = 190 x 120 x 3.5 = 79800 mm3 = 79.8 cm3 = 80 cm3 Table-2: Composition of Biocomposite Samples % of Resin % of Fiber % of Fillers A 90 10 0 B 80 10 10 C 70 10 20 D 60 10 30 Table -3: Density Calculation of Samples Mass of Resin (grams) Mass of Fiber (grams) Mass of fillers (grams) Total mass (grams) Density (g/cm3 ) 75.6 10.64 0 86.24 1.078 67.2 10.64 7.84 85.68 1.071 58.8 10.64 15.68 85.12 1.064 50.4 10.64 23.52 84.56 1.057 3. EXPERIMENTATION Mechanical tests such as tensile test, flexural test and hardness tests were conducted on the specimens prepared as per the ASTM standards. The tensile test and flexural test was conducted using J J Lloyd universal testing machine and the hardness test was conducted using Rockwell hardness testing machine. All the tests were carried out at room temperature. Fig-4: J J Lloyd UTM 3.1 Tensile Test Tensile tests on composite specimens were carried out according to ASTM–D 638 standard to determine tensile strength and to observe the behavior of biocomposites under load. Fig -5: Standard tensile specimen dimensions 3.2 Flexural Test Flexural tests on composite specimens were carried out according to ASTM D-790 standard. Fig -6: Standard flexural specimen dimensions 3.3 Rockwell Hardness Test The Rockwell hardness is determined by the depth of the indentation in the test material resulting from application of a given force on a specific indenter. 4. RESULT AND DISCUSSION In this section the results obtained from tension, Flexural and Hardness tests are tabulated and represented graphically to analyze the behavior of Biocomposites.
  • 4. IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 255 4.1 Tensile Test The peak load and tensile strength obtained from tensile test conducted on the prepared specimens is tabulated in Table -4. Table 4: Tensile Strength readings of each trail SAMPLE TRIALS PEAK LOAD (N) TENSILE STRENGTH (N/mm2) A 313 13.55 13.55 432 17.36 17.36 B 599 23.21 33.72 291 11.82 37.70 C 771 26.24 37.70 621 20.71 28.60 D 892 27.05 26.12 976 26.68 18.33 The prepared tensile test specimens before and after the tensile test experiment is shown in the figure-7 4.1.1 Effect of Peak Load From Graph 2 it can be observed that at all filler content the highest Peak Load is for 30% filler content bio- composite with a value of 934 N. Graph-2: Peak load vs Gelatin filler 4.1.2 Effect of Ultimate Tensile Strength From Graph-3 it can be observed that at all filler content the highest Ultimate Tensile Strength is for 30% filler content bio-composite with a value of 26.86Mpa. Graph-3: Tensile strength vs Gelatin filler 4.2 Flexural Test The peak load and compression strength obtained from compression test conducted on the prepared specimens is tabulated in table-5. Table -5: Flexural Strength readings of each trail SAMPLE TRIALS PEAK LOAD (N) FLEXURAL STRENGTH (N/mm2 ) A TRIAL 1 222 37.82 TRIAL 2 217 35.13 B TRIAL 1 118 23.33 TRIAL 2 102 19.77 C TRIAL 1 107 17.39 TRIAL 2 69 11.26 D TRIAL 1 31 6.13 TRIAL 2 71 12.67 The prepared flexural test specimens before and after the flexural test experiment is shown in the figure-8 Fig-8: Flexural Specimen Before and After Test
  • 5. IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 256 4.2.1 Effect of Peak Load From Graph 4 it can be observed that at all filler content the highest Peak load is for 0% filler content bio- composite with a value of 219.5N Graph-4: Peak load vs Gelatin filler 4.2.2 Effect of Flexural Strength From Graph-5 it can be observed that at all filler content the highest Flexural Strength is for 0% filler content bio- composite with a value of 36.47Mpa. Graph-5: Flexural Strength vs Gelatin Filler 4.3 Hardness Test Hardness is a surface property and is a measure of wear resistance on the surface of the composite. Hardness value increases from 0% filler content to 30%.The measured hardness number test results are shown in Table 6. Graph-6: HRBN vs Gelatin filler Table-6: Measured Hardness Number SPECIMEN COMPOSITION HRBN A Vinylester resin + Dupion silk fiber 15 B Vinylester resin + Dupion silk fiber + 10% Gelatin 20 C Vinylester resin + Dupion silk fiber + 20% Gelatin 24 D Vinylester resin + Dupion silk fiber + 30% Gelatin 28 4.4 Morphological Characteristics Below figures show the micrograph of sample revealing the morphology of Gelatin Granules and silk fiber reinforcement. Fig-9: Gelatin Granules & Silk fibers at 500 magnification Fig-10: SEM micrograph of untreated Dupion silk fiber The Micrographs reveal that the bonding between reinforcement and matrix is poor and the Gelatin granules are attracted towards the fibers since both are hydrophilic in nature.
  • 6. IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 257 5. CONCLUSION  The present work has been undertaken, with an objective to explore the potential of the natural fiber polymer Bio-composites and to study the mechanical properties of the bio-composites.  Bio-composites can supplement and eventually replace petroleum based composite materials in several applications thus offering new agricultural, environmental, manufacturing and consumer benefits.  In mechanical test, it was observed that 30% filler content has better tensile properties compared to the 0%,10%,20% filler content, the flexural strength is highest for 0% filler content, and it reduces for 10%,20%,30%.  The hardness test results shows that 30% filler content Biocomposites has better Hardness number.  In this work Tensile strength of 30% Gelatin filled Natural (Dupion silk) fiber reinforcement vinylester composite material matches the femur bone tensile strength. REFERENCES [1] H.E. Davis and J.K. Leach, Hybrid and Composite Biomaterials in Tissue Engineering, Topics in Multifunctional Biomaterials and Devices, Ed. N Ashammakhi © 2008. [2] Sangamesh Kumbar, Cato Laurencin, Meng Deng, natural and synthetic biomedical polymers (Elsevier Inc.) [3] John C. Middleton, Arthur J. Tipton, Synthetic biodegradable polymers as orthopedic devices, Biomaterials 21 (2000) 2335-2346 [4] A. Ashwin Kumar , Karthick. K, and K. P. Arumugam, Biodegradable Polymers and Its Applications, International Journal of Bioscience, Biochemistry and Bioinformatics, Vol. 1, No. 3, September 2011 [5] Dr.Shivappa, Ananda.G.K, Shivakumar.N, “Mechanical Characterization of Rice Husk Flour Reinforced Vinylester Polymer Composite", International Journal of Innovative Research in Science, Engineering and Technology, ISSN: 2319-8753, Vol. 2, Issue 11, November 2013. [6] M.SAKTHIVEl, S.RAMESH, "Mechanical Properties of Natural Fibre (Banana, Coir, Sisal) Polymer Composites", SCIENCE PARK ISSN: 2321 – 8045, Vol- 1, Issue-1, July 2013 [7] BIRSAN, I[ulian] G[abriel]; ROMAN, I[gor]; BRIA, V[asile]; UNGUREANU, V[ictor] & CIRCIUMARU, A[drian], "STARCH - EPOXY COMPOSITES", Annals of DAAAM for 2011 & Proceedings of the 22nd International DAAAM Symposium, Volume 22, No. 1, ISSN 1726-9679 ISBN 978-3-901509-83-4, 2011. [8] Shinji Ochi, "Tensile Properties of Bamboo Fiber Reinforced Biodegradable Plastics”, International Journal of Composite Materials, 2012, 1-4. [9] Munikenche Gowda T., Naidu A. C. B., and Rajput Chayya, “Some Mechanical Properties of Untreated Jute Fabric-Reinforced Polyester Composites”, Journal of Composites Part A: Applied Science and Manufacturing, vol.30, pp. 277- 284, 1999. [10] Manikandan Nair K.C., Diwan S. M., Thomas S., “Tensile properties of short sisal fibre reinforced polystyrene composites” Journal of Applied Polymer Science, New York, vol.60, pp.1483-1497, 1996. [11] Paramasivam T., Abdulkalam A. P. J., “On the study of natural fibre composites”. Fibre Science and Technology, New Delhi, vol.1, pp.85-98, 1974. [12] Shah A. N. and Lakkad S. C., “Mechanical Properties Of Jute-Reinforced Plastics”, Fibre Science and Technology vol.15 pp.41-46, 1981. [13] Pickering K.L., Li Y., Farrell R.L., & Lay M., “Interfacial modification of hemp fibre reinforced composites using fungal and alkali treatment”. Biobased Material and Bioenergy, vol.1 (1), pp.109-117, 2007. [14] Gassan J. and Bledzki A.K., “The influence of fiber-surface treatment on the mechanical properties of jute-polypropylene composites”. Composites Part-A vol.28, pp.1001-1005, 1997. [15] K. Murali Mohan Rao, K. Mohana Rao, "Extraction and tensile properties of natural fibers: Vakka, date and bamboo Composite Structures" 77 (2007) 288– 295. [16] Dr.KRDinesh1,JagadishSP, Dr. A Thimmanagouda,Dr. Neeta Hatapaki, “Characterization and Investigation of Tensile and Compression Test on Sisal Fiber Reinforcement Epoxy Composite Materials Used as Orthopaedic Implant” BIOGRAPHIES Mr. Avinash S received his B.E degree in Mechanical Engineering from VTU, India (2009), presently doing his M.E degree in advanced materials & technology at UVCE, Bangalore University, India. Presently his project is based on the field of Biomaterials, replacement of femur bone. His research interests include Composites, Nano-Technology and Cryogenics Dr. H.G.Hanumantharaju presently working as assistant professor in UVCE, Bangalore university India. He obtained his B.E degree in mechanical engineering from Mysore University, India (1991) and ME in Machine Design from UVCE, Bangalore University (2004). He obtained his Ph.D in the field of Bio-Materials from Bangalore University (2012). He has guided more than 20 ME students and currently guiding 5 research scholars. His field of interests include bio-materials, composites, stress analysis etc., Mr. Vignesh M received his B.E degree in Mechanical Engineering from VTU (2012), presently doing his M.E degree in advanced materials & technology at UVCE, Bangalore University. Presently
  • 7. IJRET: International Journal of Research in Engineering and Technology eISSN:2319-1163 | pISSN: 2321-7308 _______________________________________________________________________________________ Volume: 03 Issue: 11 | Nov-2014, Available @ http://www.ijret.org 258 his project is based on the field of Biomaterials, Functional Bone fixation Devices. His research interests include Composites, Nano-Technology, Cellular Materials and Cryogenics. Mr. Akash.S is presently working as ISAGO Trainee member at Menzies aviation Bangalore, India and completed B.E in industrial engineering and management from Sir MVIT, Bangalore (2014). His research interests include Bio-Materials and industrial relation and management.