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Lipids of physiologic significance
1. Dr. Farhana Atia
Assistant Professor
Department of Biochemistry
Nilphamari Medical College, Nilphamari
Bangladesh
Biomolecules: Lipids
2. Lipids
Lipids are heterogeneous group of compounds
that are related more by their physical than by
their chemical properties
They contain C, H, O ± P, N & S
They have common properties of being
1. Insoluble in water
2. Soluble in nonpolar solvents as ether and
benzene
3. Associated with fatty acids and utilized by
living organisms
3. Biomedical Importance
Major source of energy for the body (High caloric value=
9.3 Kcal/g)
Important dietary constituent, fat soluble vitamins &
essential fatty acid are contained in the fat of natural
food
Fat is stored in adipose tissue
Serve as thermal insulator in subcutaneous tissues &
around certain organs
Act as electrical insulator, allowing rapid propagation of
depolarization waves along myelinated nerves
Combination of lipid & protein (LP) serve as the means
of transporting lipid in blood
4. Biomedical importance
Provide hydrophobic barrier that permits partitioning of
the aqueous contents of cells & subcutaneous
structures
Some fat soluble vitamins have regulatory or coenzyme
function
Prostaglandins & steroid hormones play a major roles in
the control of the body’s homeostasis
Have essential role in nutrition & health
Imbalance of lipid metabolism may lead to some major
clinical problems
Atherosclerosis
Obesity
Diabetes Mellitus
5. Lipids: Classification
1. Simple lipid: Esters of FA with various alcohol
Fat: Esters of FA with glycerol. [Oil- in liquid state]
Waxes: Esters of FA with HMW monohydric alcohol
2. Complex lipid: Containing groups in addition to
alcohol & FA
(1) Phospholipids: Containing a phosphoric acid
residue
Glycerophospholipid [alcohol- glycerol]
Sphingophospholipid [alcohol- sphigosine]
(2) Glycolipids (glycosphingolipid): Contain FA,
Sphingosine & carbohydrate
(3) Other: Sulpholipid, Aminolipid, Lipoproteins
6. Lipids: Classification
3. Derived lipid : Formed from hydrolysis of both
simple and complex lipids.
Fatty acid
Glycerol
Steroids
Other alcohol
Fatty aldehyde
Ketone body
Hydrocarbon
Lipid soluble vitamins
Hormones
8. Fatty acids
(Aliphatic carboxylic acid)
Long hydrocarbon chain with a polar carboxyl group
at its end.
Amphipathic molecule
Polar / hydrophilic: COO⁻
Nonpolar / hydrophobic: Long HC chain
Present in body
Mainly as esters in natural fat & oils
Unesterified/ FFA [transported in plasma]
Natural FA: usually even number of C atom (14-24)
9. Fatty acids: Nomenclature
FAs are named after corresponding hydrocarbon
Numbering of C atom
• From carboxyl carbon (no. 1) - 2, 3, 4 α, β, γ
• Terminal methyl carbon- ω / n
Number & position of double bond
• ∆⁹: between 9/10 C
• ω9: On 9th counting from ω C
• 3 series of FA family: ω9, ω6, ω3
10. Fatty Acids: Classification
1. Saturated FA: No double bonds
Palmitic acid (16C), Stearic acid (18C), Lauric
acid (12C)
Source: Animal & plant fat, coconut oil, butter,
nutmeg, cinnamon, palm kernel, waxes
2. Unsaturated FA : One or more double bonds
3. Eicosanoids : Derived from eicosa (20-carbon)
polyenoic fatty acid.
11. Unsaturated Fatty acids
1. Monounsaturated FA: one double bond.
• Oleic acid (18:1;9)
• Source: nearly all fats, high in olive oil
2. Polyunsaturated FA (PUFA): 2/more double bonds.
• Linoleic (18:2;9,2)
• α-Linolenic (18:3;9,12,15)
• Arachidonic (20:4;5,8,11,14)
• Cervonic (22:6;4,7,10,13,16,19)
• Source: Corn, peanut, cotton seed, soy bean,
linseed oil, Fish oil (cod liver, salmon oil),
phospholipids in brain
12. Fatty Acids: Classification
Eicosanoids: Comprises-
Prostanoides
Prostaglandins/ PGs
Prostacyclins/ PGIs
Thromboxanes/ TXs
Leukotreins/ LTs
Lipoxins/ LXs
PGs exist in all mammalian
tissue
Synthesized in vivo
According to Chain length
fatty acids are-
Short chain FA: 2-10
carbon.
Acetic acid (2C), butyric
acid (4C)
Long chain FA: >10
carbon.
Palmitic (16C), stearic
(18C)
13. Essential fatty acids
Polyunsaturated fatty acids
Human body is unable to synthesize
Must be supplied in diet
Linoleic acid, ω₆ FA , ( AA Prostaglandins)
α-Linolenic acid, ω₃ FA (Important for growth &
development)
Arachidonic acid, (ω₆ FA), become essential if
linoleic acid is absent in diet
14. Essential fatty acids
Maintain fluidity of membrane structure
Deficiency rare, occur in
• Infant on formula diet
• On IV nutrition exclusively for long time
Result in
• Scaly dermatitis (icthyosis)
• Hair loss
• Visual abnormality
• Neurologic abnormality
• Poor wound healing
15. ω₆/n₆ Family
From vegetable oil: nuts,
avocado, olive, soy bean,
sesame, cotton seed, corn
↓plasma cholesterol
↓ LDL, also ↓HDL
Excess intake (↑PUFA)
oxidation of FA ↑free
radicals
Cancer
Inflammatory Disease
Atherosclerosis
Aging
ω₃ / n₃ Family
From plants: linseed oil
Fish oil (DHA & EPA)
Have anti-inflammatory
effect. So beneficial in
cardiovascular disease
Cancer
Rheumatoid arthritis
Alzheimer disease
In infant- helps in brain
development
16. Eicosanoids
Prostaglandins & related compounds, thromboxanes &
leukotriens are collectively known as eicosanoids
Origin: PUFA with 20C
Extremely potent compounds, elicit a wide range of
responses
• Physiologic [inflammatory]
• Pathologic [hypersensitivity]
Functionally compared to hormone, but
Produce in small amount in all tissue
Act locally
Not stored, extremely short half life
Action mediated by plasma mem. G protein coupled receptor
18. Functions of Eicosanoids
PGF₂α
Produce by- most tissue
Vasoconstriction
Contraction of smooth
muscle
Uterine contraction
PGE₂
Produced-most tissue, kidney
Vasodilatation
Relax smooth muscle
Used to induce labour
PGI₂
Produced in endothelium
Vasodilatation
Inhibit platelet aggregation
TXA₂
Produced by platelets
Promote platelet
aggregation
Vasoconstriction
Mobilize IC calcium
Contraction of smooth
muscle
19. LTA₄
Produce- leukocytes, platelets,
mast cell, heart & lung vascular
tissue
LTC₄/ LTD₄/ LTE₄
Contraction of smooth muscle
Broncho-constriction
Vasoconstriction
↑vascular permeability
Make up slow reacting
substances [SRS] of anaphylaxis
Involved in pathophysiology of
asthma
LTB₄
↑ chemotaxis of
PMN leukocytes
Release of lysosomal
enzyme
Adhesion of WBC
LXs
Anti-inflammatory
role
Immuno-regulatory
function
20. Triacylglycerol
Esters of trihydric
alcohol and fatty acids
Glycerol : Alcohol with
3 OH groups
Main storage forms of
fatty acids
Thermal insulator
21. Phospholipids
Esters of fatty acids, which contain an alcohol &
one or more fatty acids and a phosphoric acid
residue.
They frequently have nitrogen containing bases
(choline) & other substituents
Glycerophospholipid
[alcohol- glycerol]
Sphingophospholipid
[alcohol- sphigosine]
22. Glycerophospholipids Sphingophospholipids
Phosphatidylcholine (lecithin)
Most abundant PL in membrane
Acetylcholine
Dipalmitoyl lecithin (surfactant)
Phosphatidyl ethanolamine
(cephalin)
Phosphatidyl serine
Phosphatidyl inositol (2nd
messenger)
Plasmalogens (Brain, heart)
Cardiolipins (mitochondria)
Spingomyelins
Component of cell
membrane, nervous
tissue and myelin
sheath
Play role in cell
signaling & apoptosis
Ceramide:
combination of
sphingosine plus fatty
acid
23. Biomedical Importance of Phospholipids
They are main lipid constituents of cell membrane
Provide arachidonic acid for eicosanoids
Surfactant- prevent adherence of inner surface of lung
Play an important part in cell signaling & membrane
trafficking
Keep cholesterol in solution (in bile)
Play role in apoptosis (programmed cell death)
Acetylcholine is important in nervous transmission
Cardiolipin- decrease level cause heart failure,
hypothyroidism
Plasmalogens may have a protective effect against reactive
oxygen species
24. Glycolipids
Glycosphingolipids
Contain a fatty acid, sphingosine, and carbohydrate
Widely distributed in every tissue (particularly in brain)
Example: Cerebrosides, Gangliosides
Biomedical Importance
Component of biological membrane
Present in brain, other nervous tissue & myelin
Cell surface receptor (glycocalyx)
Cellular communication, cell-cell recognition
Receptors for hormone & bacterial toxins (cholera toxin)
25. Steroids
Derived lipid
Complex molecule consisting of four
fused carbon ring (17C)
Phenanthrene nucleus: Three 6-
carbon ring (A,B, C)
Cyclopentane: D ring
10, 13: methyl; 17: side chain
Common cyclic ring called “steroid
nucleus”/
cyclopentanoperhydrophenanthrene
ring
26. Important steroids include
A. Bile acids and salts
B. Adrenocortical hormones
C. Sex hormones
D. Vitamin D
E. Cardiac glycosides
Steroids
Bile salt
27. Sterols
If the steroid has 1/ more OH groups & no
carbonyl/ carboxyl group, it is a sterol. The name
terminates in –ol.
Cholesterol, ergosterol, sitosterol, coprosterol
Cholesterol : Major sterol in animal tissue
3: OH; 17: Aliphatic side
Ergosterol: In plant & yeast, precursor of vitamin D
28. Cholesterol
Cholesterol is the major sterol in animal tissues.
Body cholesterol-
50% Synthesized in body (700 mg/d)
50% from Diet
70 kg man: 140 gm cholesterol
Source:
Egg yolk
Meat
Liver
Brain
[No vegetable oil contains any cholesterol]
29. Biomedical importance
Cholesterol is widely distributed in all cells of the body
but particularly in nervous tissue
It is an amphipathic lipid & is an essential structural
component of cell membrane. It is important for the
maintenance of correct permeability & fluidity
Major constituent of plasma lipoprotein
Present in tissue & plasma as free/ combined with a
LCFA as cholesteryl ester (storage form)
Occurs in animal but not in plants or bacteria
Precursor of all steroids in body including
corticosteroids, sex hormones, bile acids, vitamin D
30. Plasma LDL is the vehicle that supplies cholesterol &
cholesteryl ester to many tissues
Free cholesterol is removed from the tissues by
plasma HDL & transported to liver.
It is eliminated from the body either unchanged or
after conversion of bile acids (reverse cholesterol
transport)
Cholesterol is a major constituent of gall stone
Chief factor in the genesis of atherosclerosis and
heart disease
Biomedical importance