Ezhil2.Ppt

ISOLATION OF HUMAN FETAL LIVER STEM CELLS WITH ALFA FETO PROTEIN, CK18 & ALBUMIN Presented by S. EZHIL MANGAI

[object Object],INTERNAL GUIDE : L.G. MOHANA DIVYA Lecturer Department Of Biotechnology KSR College Of Technology EXTERNAL GUIDE : DR. ALEEM KHAN Centre For Liver Research And Diagnostics Hyderabad.

INTRODUCTION ,[object Object],[object Object],[object Object],[object Object]

OBJECTIVE ,[object Object],[object Object],[object Object]

VIABILITY ASSAY ,[object Object],[object Object],[object Object]

IMMUNOCYTOCHEMISTRY ,[object Object],[object Object],[object Object],[object Object]

PROCEDURE ,[object Object],[object Object],[object Object]

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MTT ASSAY ,[object Object],[object Object]

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PROCEDURE ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

RESULTS ,[object Object],[object Object],[object Object],[object Object]

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MTT ASSAY Absorbance of MTT Formazan, the Standard Procedure to Access Cell Activity: 0.9945 250 1.1436 300 0.8398 200 0.6240 150 0.4421 100 0.2621 50 0.0000 Blank OD at 580 nm Concentration of cell activity (µg/ml)

Estimation of cell activity by MTT assay: 0.1790 39 0.1349 35 OD at 540 nm Percentage of activity (µg/ml)

WORK TO BE DONE ,[object Object],[object Object],[object Object],[object Object],[object Object]

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