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First Chapter

Plant Cells

one Form and Structure of Plant Cells
★ Definition
The structural and functional unit of a tissue ,
and consequently of the plant body itself , is known
as a cell . Some species or kind of plants consist of
single cell , but large type are make up of
uncountable millions , or even billions , of these
structural units.
Chlamydomonas

Nostoc commune Vauch
1.Shape and Size of Plant Cells
It has various shapes and sizes.


Cell , which distribute in differ part of frond and
perform differ function , has differ form and size .
plant cell , commonly 10 ~ 100μm(diameter)


least cell , as if
bacterial diameter only
0.2μm



Big cell , like cell of
storage parenchyma
1mm



Fiber cell of flax 4cm
long



Fiber of ramee 55cm
long
2.Basic Structure and Function
of Plant cells
Definition
Microscopic structure: there can be seen the cell

structure with microscope, called microscopic
structure.

Submicroscopic structure: there can be seen the cell

structure with electron microscope , termed
submicroscopic structure.
Microscopic structure

1. nuclear
membrane
2. nucleolus
3.chromosome
4. Cell wall
5. plasmalemma
6. vacuole

membrane
7. vacuole
8. Chloroplast
9.Mitochondria
10.microtuble
11. Endoplasmic

submicroscopic
1.Cell wall 2.nuclear membrane
structure
3.nucleochylema 4.nucleolus
5.plasmalemma 6.Cytoplasm
7.vacuole membrane 8.chloroplast
9.vacuole

reticulum
12.Ribosome
13.14.microbody15.
golgi body
一、 cell wall

★ constitute

二、 protoplast
三、 ergastic substance
Intercellular Layer :
It is thin layers owned in common between cell
wall and cell wall .
Primary Wall:
By cellulose , hemicellulose and pectin material
that protoplasm secrete, increase in interior side of
intercellular layer.
Secondary Wall:
After the cell wall stops growing, are
accumulating some materials coming into being in
the interior of the primary wall on each gradually,
make the cell wall increase and take shape on
concentric storey thick.


Ⅰ. pit  


simple pit
bordered pit
half bordered pit

 Ⅱ.plasmodesmata
 Pit
 The

secondary wall does not increase thick

evenly , leave some and has not increased
the thick part in a lot of places during the
process of thickenning, called the pit.
Ⅰ.Simple pit Ⅱ.bordered pit Ⅲ.half boredered pit
1.tangent plane view 2.exterior view
Bordered pit of pine’s stem and Radix et Rhizoma Rhei
 Ⅱ.plasmodesmata


There are a lot of very
thin protoplasm silks that
cross and come into
being the very small hole
on the primary wall and
get in touch each other
among the cells, this kind
of protoplasm silk is
called plasmodesmata.
Plasmodesmata of Persimmon core


 Ⅲ.



Specification of Cell Walls

lignification
suberization
cutinization
mucilagization
mineralization
 1.lignification:

The cell wall has increased
more lignin while adding and growing.
①Distinguish: it was stained Red when
adding meta benzene and three phenol +
dense hydrochloric acid is.
 ②Example: vessel , wood fiber , stone cell.


2. suberization: Have increased the fat phellem in
the cell wall.



①Distinguish :it was dyed Red when adding
Sudan Ⅲ test solution(TS) is.



②Example: Brown outer bark trunk outside.
 3.

cutinization : Fat cutin except not being

pack the cell wall , form a layer of cuticles
outside epidermis of stem often.
 ①Distinguish

:it was dyed when adding

Sudan Ⅲ test solution is orange.
 ②Example:

The seed of the castor-oil

plant , stem peel of the sugarcane.
 4.mucilagization:

Cellulose and such

composition as the pectin ,etc. in the cell
wall change but forms the mucus .
 ①Distinguish

:it was stained Red when

adding Red reagent of ruthenium .
 ②Example:

In the epidermis cells of

plantain , linseed.
 5.

mineralization: Contain the silicon or the

calcium ,etc. in the cell wall.
 ①Distinguish

:The silicon can be dissolved

in the hydrogen fluoride(HF), but does not
dissolve in the acetic acid or denser
sulphuric acid .
 ②Example:Gramineous

Stem and leaf



②protoplast

cytoplasm
nucleus




nuclear membrance
nuclear sap
nucleolus
chromatin



plastids



Organelle



chloroplast
leucoplast
chromoplast

vacuoles



 There

are all life matter in the cell,
called protoplast .
 Chloroplast:green

little grains(leaf)
 leucoplast:achromatous and global
grains (immature organ )
 Chromoplast:anomalous red,yellow,and
orange grains(flower,fruit,seed)
 Three kinds of plastid can convert
mutually.
 Vacuoles:there are many little vacuoles
in the delicate cell , but in mature ones
there is only one big vacuole can be
seen which occupied most part of the
cell.
Mutually converting of plastic
 ③Ergastic


Substance

The vegetable cell in the life process, produces
various non- life matter as a result of the
metabolism activity, called Ergastic
Substance.

 Ⅰ.starch grain: hilum , annular striation

lamellae
reserve starch:
①simple starch grain
②compound starch grain
③half compound starch grain
1.2.3.4.simple starch grain
(1.lambdoidal hilum 2.short gap)
5.compound starch grain
 Ⅱ.Inulins
 Fan-shaped

and yellow



Ⅲ.Proteins(aleurone grain)
Those are composed together by protein crystal ,
spherical albuminous body and amorphous
stuffed gelatin. Add one drop iodine-potassium
iodide TS , the protein turns yellow.
 Ⅳ.Fats

and Fatty Oils

several drops of Sudan Ⅲ TS( 试液 ) oil
droplet contained in the cell stained red.

 Add
 Ⅴ.Crystals
①


calcium oxalate crystal

solitary crystal : square-shaped, diamond,
rectangle or double-conical octahedrons


acicular crystal : scattered or needle-like
cluster
 cluster

crystal: asteroid or clusters
 crystal

sand: irregular, polyhedrons
 columnar

crystal : long is cylindrical, the
length approximately is above diameter 4
times
②

calcium carbonate crystal

 (cystolith):

like a string of grape [locate in

Moraceae or Acanthaceae ]
Calcium carbonate crystal
Ⅰcysyolith ( tangent plane view ) in fig leaf
Ⅱ exterior view
 Besides

, physiological active substance

like enzyme , vitamin , auxin , antibiotic ,
tannins , etc.
Two Division of Plant cells
 Cell

division is necessary and play
important role for embryo to little frond to
mature frond( possessing root , stem and
leaf , flower , fruit , seed).

 Three

types:( 一 )mitosis
( 二 )amitosis ( 三 )meiosis
㈠

mitosis

 ①interphase

: the cytoplasm is denser ,

the nucleus is big , nuclear menbrane with
the nuclear sap can be seen clearly.
 ②prophase
 There

inside the nucleus emerge
condensed granulose chromatin and then
gradually become the clubbed
chromosome.
 ③metaphase:

the nuclear membrane , the
nuclear sap disappear , the chromosome
and the spindle fibers connect with each
other , and arrange in the surface of the
equator.
 ④anaphase:

the centromere detaching
,each pair of chromatids set apart from the
equatorial plate to move to the two poles,
thereby to compose the independent
daughter chromosome respectively.
 ⑤telophase

: the two daughter
chromosomes arrive at the two poles ,
chromosome gradually fall into slender ,
emerging of the nuclear membrane with
nuclear sap again . Meanwhile , divide into
two cells in the two poles.
Mitosis
1.Interphase 2-4.prophase 5.metaphase
6-7.anaphase 8.telophase 9.two daughter cells
㈡


amitosis

Direct nuclear division and it is most simple
and common type of division . (in lower plant)
㈢

meiosis

 one especial mitosis , as a result , divide
into four cells in process of sexual
reproductive.
 Review

Questions

一

.Definition
 Pit , plasmodesmata , protoplast , ergastic
substance
二

.simple answer
 1.How about the composing of the cell wall?
 2.what are included in cell wall
characteristics?
 3.which types has crystal?

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1. plant cell

  • 1. First Chapter Plant Cells one Form and Structure of Plant Cells ★ Definition The structural and functional unit of a tissue , and consequently of the plant body itself , is known as a cell . Some species or kind of plants consist of single cell , but large type are make up of uncountable millions , or even billions , of these structural units.
  • 3. 1.Shape and Size of Plant Cells It has various shapes and sizes.  Cell , which distribute in differ part of frond and perform differ function , has differ form and size . plant cell , commonly 10 ~ 100μm(diameter)
  • 4.  least cell , as if bacterial diameter only 0.2μm  Big cell , like cell of storage parenchyma 1mm  Fiber cell of flax 4cm long  Fiber of ramee 55cm long
  • 5. 2.Basic Structure and Function of Plant cells Definition Microscopic structure: there can be seen the cell structure with microscope, called microscopic structure. Submicroscopic structure: there can be seen the cell structure with electron microscope , termed submicroscopic structure.
  • 6. Microscopic structure 1. nuclear membrane 2. nucleolus 3.chromosome 4. Cell wall 5. plasmalemma 6. vacuole membrane 7. vacuole 8. Chloroplast 9.Mitochondria 10.microtuble 11. Endoplasmic submicroscopic 1.Cell wall 2.nuclear membrane structure 3.nucleochylema 4.nucleolus 5.plasmalemma 6.Cytoplasm 7.vacuole membrane 8.chloroplast 9.vacuole reticulum 12.Ribosome 13.14.microbody15. golgi body
  • 7. 一、 cell wall ★ constitute 二、 protoplast 三、 ergastic substance
  • 8. Intercellular Layer : It is thin layers owned in common between cell wall and cell wall . Primary Wall: By cellulose , hemicellulose and pectin material that protoplasm secrete, increase in interior side of intercellular layer. Secondary Wall: After the cell wall stops growing, are accumulating some materials coming into being in the interior of the primary wall on each gradually, make the cell wall increase and take shape on concentric storey thick.
  • 9.  Ⅰ. pit    simple pit bordered pit half bordered pit  Ⅱ.plasmodesmata
  • 10.  Pit  The secondary wall does not increase thick evenly , leave some and has not increased the thick part in a lot of places during the process of thickenning, called the pit.
  • 11. Ⅰ.Simple pit Ⅱ.bordered pit Ⅲ.half boredered pit 1.tangent plane view 2.exterior view
  • 12. Bordered pit of pine’s stem and Radix et Rhizoma Rhei
  • 13.  Ⅱ.plasmodesmata  There are a lot of very thin protoplasm silks that cross and come into being the very small hole on the primary wall and get in touch each other among the cells, this kind of protoplasm silk is called plasmodesmata. Plasmodesmata of Persimmon core
  • 14.    Ⅲ.   Specification of Cell Walls lignification suberization cutinization mucilagization mineralization
  • 15.  1.lignification: The cell wall has increased more lignin while adding and growing. ①Distinguish: it was stained Red when adding meta benzene and three phenol + dense hydrochloric acid is.  ②Example: vessel , wood fiber , stone cell.
  • 16.  2. suberization: Have increased the fat phellem in the cell wall.  ①Distinguish :it was dyed Red when adding Sudan Ⅲ test solution(TS) is.  ②Example: Brown outer bark trunk outside.
  • 17.  3. cutinization : Fat cutin except not being pack the cell wall , form a layer of cuticles outside epidermis of stem often.  ①Distinguish :it was dyed when adding Sudan Ⅲ test solution is orange.  ②Example: The seed of the castor-oil plant , stem peel of the sugarcane.
  • 18.  4.mucilagization: Cellulose and such composition as the pectin ,etc. in the cell wall change but forms the mucus .  ①Distinguish :it was stained Red when adding Red reagent of ruthenium .  ②Example: In the epidermis cells of plantain , linseed.
  • 19.  5. mineralization: Contain the silicon or the calcium ,etc. in the cell wall.  ①Distinguish :The silicon can be dissolved in the hydrogen fluoride(HF), but does not dissolve in the acetic acid or denser sulphuric acid .  ②Example:Gramineous Stem and leaf
  • 21.  Chloroplast:green little grains(leaf)  leucoplast:achromatous and global grains (immature organ )  Chromoplast:anomalous red,yellow,and orange grains(flower,fruit,seed)  Three kinds of plastid can convert mutually.  Vacuoles:there are many little vacuoles in the delicate cell , but in mature ones there is only one big vacuole can be seen which occupied most part of the cell.
  • 23.  ③Ergastic  Substance The vegetable cell in the life process, produces various non- life matter as a result of the metabolism activity, called Ergastic Substance.  Ⅰ.starch grain: hilum , annular striation lamellae reserve starch: ①simple starch grain ②compound starch grain ③half compound starch grain
  • 24. 1.2.3.4.simple starch grain (1.lambdoidal hilum 2.short gap) 5.compound starch grain
  • 26.   Ⅲ.Proteins(aleurone grain) Those are composed together by protein crystal , spherical albuminous body and amorphous stuffed gelatin. Add one drop iodine-potassium iodide TS , the protein turns yellow.
  • 27.  Ⅳ.Fats and Fatty Oils several drops of Sudan Ⅲ TS( 试液 ) oil droplet contained in the cell stained red.  Add
  • 28.  Ⅴ.Crystals ①  calcium oxalate crystal solitary crystal : square-shaped, diamond, rectangle or double-conical octahedrons
  • 29.  acicular crystal : scattered or needle-like cluster
  • 32.  columnar crystal : long is cylindrical, the length approximately is above diameter 4 times
  • 33. ② calcium carbonate crystal  (cystolith): like a string of grape [locate in Moraceae or Acanthaceae ]
  • 34. Calcium carbonate crystal Ⅰcysyolith ( tangent plane view ) in fig leaf Ⅱ exterior view
  • 35.  Besides , physiological active substance like enzyme , vitamin , auxin , antibiotic , tannins , etc.
  • 36. Two Division of Plant cells  Cell division is necessary and play important role for embryo to little frond to mature frond( possessing root , stem and leaf , flower , fruit , seed).  Three types:( 一 )mitosis ( 二 )amitosis ( 三 )meiosis
  • 37. ㈠ mitosis  ①interphase : the cytoplasm is denser , the nucleus is big , nuclear menbrane with the nuclear sap can be seen clearly.
  • 38.  ②prophase  There inside the nucleus emerge condensed granulose chromatin and then gradually become the clubbed chromosome.
  • 39.  ③metaphase: the nuclear membrane , the nuclear sap disappear , the chromosome and the spindle fibers connect with each other , and arrange in the surface of the equator.
  • 40.  ④anaphase: the centromere detaching ,each pair of chromatids set apart from the equatorial plate to move to the two poles, thereby to compose the independent daughter chromosome respectively.
  • 41.  ⑤telophase : the two daughter chromosomes arrive at the two poles , chromosome gradually fall into slender , emerging of the nuclear membrane with nuclear sap again . Meanwhile , divide into two cells in the two poles.
  • 43.
  • 44. ㈡  amitosis Direct nuclear division and it is most simple and common type of division . (in lower plant)
  • 45. ㈢ meiosis  one especial mitosis , as a result , divide into four cells in process of sexual reproductive.
  • 46.
  • 47.  Review Questions 一 .Definition  Pit , plasmodesmata , protoplast , ergastic substance 二 .simple answer  1.How about the composing of the cell wall?  2.what are included in cell wall characteristics?  3.which types has crystal?