The document summarizes a study on using an HPV mRNA test to detect high-risk HPV infections in 302 patients. The study found the mRNA test had a low invalid rate and provided useful results. In patients with abnormal cytology results of ASCUS or LSIL, 66% and 56% respectively tested negative for high-risk HPV using the mRNA test. The test also detected active HPV infections in 10 out of 48 patients with unclear cytology results. The mRNA test was found to be a fast and reliable method for HPV detection and typing.
ỨNG DỤNG CHẨN ĐOÁN PHÂN TỬ TRONG NHÓM BỆNH UNG THƯ
HPV mRNA Test Detects Active Infections in 302 Patients
1. Molecular diagnostics of HPV infection using E6 and E7 mRNA detection: experience in 302 patients Jeremie M. Gras, M.D. Clinical and Molecular laboratory Cliniques Sud Luxembourg, Arlon, Belgium bioMérieux Satellite Symposium Eurogin Congress, Monte-Carlo, 18th February 2010
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11. 2. Patients and Methods Methods 1. Cytology first performed, then PreservCyt® vial was transmitted to our lab for preparation (40 mins) 2. Extraction on the easyMAG ® (40 mins) 3. Incubation (40 mins) 4. HPV mRNA amplification and detection using the EasyQ ® test ( NASBA ) (120 mins) TAT for 24 samples: 4 hours
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13. 3. Results Overall Low rate of invalid results
14. 3. Results ASCUS ASCUS: 66% have a negative HPV test
15. 3. Results L-SIL L-SIL: 56% have a negative HPV test
16. 3. Results H-SIL Negative samples have been retested with a DNA method N.B: HPV 61 and 81 are not HR HPVs
17. 3. Results Unclear cytology (N=48) 10 active infections with HR HPVs
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24. Thanks Molecular Biology Laboratory, Cliniques Sud Luxembourg ARLON, Belgium Nicolas Hougardy Head Molecular Lab Pierre Goffinet Lab Director Brigitte Léger Nathalie Pinon Bernadette Legère MLTs Andrée Lemaire Françoise Soblet Maggy Conrardy
26. Molecular diagnostics of HPV infection using E6 and E7 mRNA detection: experience in 302 patients Jeremie M. Gras, M.D. Clinical and Molecular laboratory Cliniques Sud Luxembourg, Arlon, Belgium bioMérieux Satellite Symposium Eurogin Congress, Monte-Carlo, 18th February 2010
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