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DEVELOPMENT OF
  IMPROVED COCOA
CLONES FOR FARMERS:
    THE CASE OF
     PHILIPPINE
  CLONES/HYBRIDS

 ROMULO L. CENA, Ph.D.
    University of Southern Mindanao
    Kabacan, Cotabato, Philippines
        Landline: +63642482426
           Mobile #639207338887
         romulo55cena@yahoo.com
FACTS AND FINDINGS
 Around 10,000 to 15,000 cacao farmers
  nationwide.
 Annual consumption in beans equivalent is
  about 25,000 MT
 Average cacao beans production: 6-7 T MT
 Current average yield per tree is 1.30 kg dried
  beans.
 Average value of imports: USD 42M, chocolate
  products and consumable not included
 Average value of exports:
  USD 3.5 million
 Major growing regions are :
  Davao      Region,    Northern
  Mindanao, CARAGA, Western
  Mindanao,       and    Eastern
  Visayas.
 Davao Region produced 77%
  of cacao beans supply in the
  last 5 years (2006-2011).
A. Germplasm Collection
1. Eighty-eight (88) accessions collected
   all over the country.
2. Twenty-five (25) collected from abroad.
3. Twelve (17) new hybrids introduced
   into the country through the INGENIC
   Asia- Pacific Program for Cocoa.
4. Coordinated and financed by MARS
   Inc., and World Cocoa Foundation
   (WCF)
Germplasm collection
• 125 total accessions
  maintained
  – Forastero
  – Trinitario
  – Criollo
  – Theobroma bicolor
  – 100 clones to be
    requested by the
    University of
    Reading
Accession     Clone      Accession    Clone      Accession    Clone
   No.                                              No.
                            No.
Ccr-001       UF-42      Ccr-0016    Catongo     Ccr-0031     BR-25
Ccr-002      EET-376     Ccr-0017    UIT/PA7     Ccr-0032      S-5
Ccr-003       ICS-8      Ccr-0018     ICS-60     Ccr-0033    GU-235Q
Ccr-004       UF 650     Ccr-0019     ICS-40     Ccr-0034    UF-676
Ccr-005       EET-94     Ccr-0020     UF-29      Ccr-0035    GU-133P
Ccr-006       CC-18      Ccr-0021     EET-19     Ccr-0036    GU-258P
Ccr-007        R-117     Ccr-0022     SCA-12     Ccr-0037    GU-228P
Ccr-008       GA-11      Ccr-0023       P-7      Ccr-0038    GU-123P
Ccr-009       SCA-94     Ccr-0024     SPA-4      Ccr-0039    GU-414E
Ccr-0010    Amelionad0   Ccr-0025      R-29      Ccr-0040     CC-44
Ccr-0011      SCA-6      Ccr-0026      SIAL      Ccr-0041     SPA-9
Ccr-0012      SIC-12     Ccr-0027     UF-242     Ccr-0042    SCA-49
Ccr-0013      NA-36      Ccr-0028     UF-613     Ccr-0043      C-7
Ccr-0014      ICS-6      Ccr-0029    Forastero   Ccr-0044    UF-667
Ccr-0015      EET-12     Ccr-0030     A-246      Ccr-0045    THS-64
Cacao clones to be acquired this
            year 2013
100 clones of cacao to be acquired from the
International Cocoa Quarantine Center
UF18    M01           K2




 TS03    Cr-01
                 PBC 123
B. Characterization using morphological
  markers
C. Characterization using molecular markers
D. Conduct Genetic Diversity
  Analysis as basis for Hybrid
           Breeding

1. Morphological analysis

2. Isozyme analysis

3. Molecular marker analysis
A. Breeding of Desirable Cacao Germplasm

 a.   Selection of parents
 b.   Emasculation/hybridi
      zation
 c.   Evaluation of hybrids
 d.   Selection of desirable
      hybrid plant
 e.   Clonal evaluation
Crosses Made:
 1. BR 25 x K2    8. P 1 x P8
 2. BR 25 x K1    9. UF 18 x BR 25
 3. BR 25 x S5   10. UF x K1
 4. K2 x K1      11. UF x K2
 5. K1 x K2      12. UF x S5
 6. BR 25 x P1   13. UF x PBC 123
 7. BR 25 x P8   14. S5x K2
BR 25 X K2
BR 25 X K2
BR 25 X K1
PARTICIPATING COUNTRIES
 USM, Philippines
 MCB, Malaysia
 ICCRI, Indonesia
 CCI, Papua New Guinea
 NLU, Vietnam
 CPCRI, India
Hybrids harvested this 2nd of November 2012.
Table 1. Kind crosses, no. of flowers pollinated, no. of fruits
         developed and percentage (%) success pollination of cacao.
       CROSSES         No. of flowers   No. of fruits   Percentage of success
  Female       Male     pollinated      developed          pollination (%)

  UF 18        BR 25        186              10                  5.4
  UF 18          K2         206              45                 21.8
  UF 18          K1         152              31                 20.3
  UF 18     PBC 123         188              30                 15.9
  UF 18          S5         154              8                   5.2
  S5             K2         20               5                   25
  K9             S5         30               7                  26.5
       TOTAL               936              136
Philippine cacao hybrid seedlings
transplanted in the field (April 2012)
Vietnam Clones
Papua New Guinea Hybrids
                           Indonesian Hybrids
PNG Cacao
                                     Hybrid
                                     Trees



                                                               KEE 42 X K 82
KEE 42 X K72-124




                   KEE 42 X K 82               KEE 42 X K 82
MALAYSIAN HYBRIDS




                  PBC 123 x LAFI 7




                    PBC 123 x LAFI 7
QH22 x NA 33
Table 2. Number of plants, no. of plants with fruits, stem
          girth and jorquette height of two hybrids from
          PNG and one hybrid from Malaysia planted in
          2009 at USM, Kabacan, Cotabato.
                                                  No. of
                                   No. of         plants   Stem Girth      Jorquette
               Crosses
                                   plants          with       (cm)        Height (cm)
                                                  fruits
       Female         Male
    1. KEE 42       K72-124          30             5        14.2                114.9
    2. KEE 42       K82              34             4        16.0                119.0
    3. MCBC 3       KKM 22           1              1        19.4                137.0




KEE 42 X K72- 124             KEE 42 X K72- 124              KEE 42 X K72- 124
Table 3. Dry bean data of cacao crosses coming from
         Malaysia and PNG.
       Crosses           Length of   Width of Thickness   Weight of
                           bean       bean      (cm)       100 dry
    Malaysia               (cm)       (cm)                beans (g)
  QH 22 X NA 33            24.6        1.3       0.8        105.9
 PBC 123 X QH 22           25.0        1.3       1.0        127.2
 PBC 123 X LAFI 7          24.6        1.3       1.0        130.6
 CCI, Papua New Guinea

 KEE 42 x K72-124          26.6        14.0      1.0       127.1
   KEE 42 x K 82           24.0        13.4      0.8       120.4
      Control
      BR 25                23.0        1.3      0 .9       106.3
      UF-18                26.0        1.4      0. 9       141.3
Three cacao crosses from Malaysia




                     QH 22 X NA 33
                                     PBC 123 X QH22
Cocoa Pod Borer (Conopomorpha cramerella)
                                       Life Cycle (3‐5 weeks)




                    Adult(3‐7 days)


Pupa (5‐7 days)



                                          Egg (3‐7 days)




Emerging Larva    Larva (14‐18 days)
1. Development of Artificial Diet for Cocoa Pod Borer




                                Video file (double click)
2.Vascular Streak Die Back and Pod
Rot Studies
VSD
3. Research at the nursery level
• Effect of different size of polybag on the
  growth of UF18 cacao seedling
• Effect of different media combination on the
  growth of UF18 cacao seedling
• Study on different propagation methods
4. Intercropping Cacao under 20-year old
             rubber plantation.
5.     Determination of
Theobromine Content,
Butterfat content, etc.
Sample of cacao pods of NSIC approved
varieties

           S5




           K2
                       K1
1. Establishment of Budwood Garden in Strategic
   Location in the Philippines

•   USM, Kabacan, Cotabato         •   Naga City
•   Sta. Maria, Davao del Sur      •   Lambunao, Iloilo City
•   San Isidro, Davao del Norte    •   Coner, Apayao
•   Mati, Davao Oriental           •   Sorsogon
•   Ninoy Aquino, Sultan Kudarat
•   Zamboanga City
•   Zamboanga Sibugay
•   Dipolog, Zamboanga del Norte
•   Brookes Point, Palawan
•   Puerto Princesa, Palawan
2. Survey, Identification and Tagging of Existing
   Budwood Garden
Summary
• 125 accessions maintained in our
  genebank
• Breeding of cacao and others studies in
  going-on.
• Exchange of hybrids with 5 other countries
  Malaysia, Indonesia, PNG, Vietnam,India
. 10 NSIC approved varieties of cacao in
  the Philippines
.
SUMMARY
• 3 more new varieties indorse by the NSIC
  secretariate yesterday to the seed council.
     PBC 123, USM CH 01, USM CH 02.

 . Seedling Available for Sale in Cacao
  Nurseries all over Mindanao are mostly
  UF 18, BR 25, K2, K9 ande K1.
Pure Tablea. Chokolate de
Universidad. PICRI, USM,
Kk day 2 am 2nd speaker dr. romy cena

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Kk day 2 am 2nd speaker dr. romy cena

  • 1. DEVELOPMENT OF IMPROVED COCOA CLONES FOR FARMERS: THE CASE OF PHILIPPINE CLONES/HYBRIDS ROMULO L. CENA, Ph.D. University of Southern Mindanao Kabacan, Cotabato, Philippines Landline: +63642482426 Mobile #639207338887 romulo55cena@yahoo.com
  • 2. FACTS AND FINDINGS  Around 10,000 to 15,000 cacao farmers nationwide.  Annual consumption in beans equivalent is about 25,000 MT  Average cacao beans production: 6-7 T MT  Current average yield per tree is 1.30 kg dried beans.  Average value of imports: USD 42M, chocolate products and consumable not included
  • 3.  Average value of exports: USD 3.5 million  Major growing regions are : Davao Region, Northern Mindanao, CARAGA, Western Mindanao, and Eastern Visayas.  Davao Region produced 77% of cacao beans supply in the last 5 years (2006-2011).
  • 4.
  • 5. A. Germplasm Collection 1. Eighty-eight (88) accessions collected all over the country. 2. Twenty-five (25) collected from abroad. 3. Twelve (17) new hybrids introduced into the country through the INGENIC Asia- Pacific Program for Cocoa. 4. Coordinated and financed by MARS Inc., and World Cocoa Foundation (WCF)
  • 6. Germplasm collection • 125 total accessions maintained – Forastero – Trinitario – Criollo – Theobroma bicolor – 100 clones to be requested by the University of Reading
  • 7. Accession Clone Accession Clone Accession Clone No. No. No. Ccr-001 UF-42 Ccr-0016 Catongo Ccr-0031 BR-25 Ccr-002 EET-376 Ccr-0017 UIT/PA7 Ccr-0032 S-5 Ccr-003 ICS-8 Ccr-0018 ICS-60 Ccr-0033 GU-235Q Ccr-004 UF 650 Ccr-0019 ICS-40 Ccr-0034 UF-676 Ccr-005 EET-94 Ccr-0020 UF-29 Ccr-0035 GU-133P Ccr-006 CC-18 Ccr-0021 EET-19 Ccr-0036 GU-258P Ccr-007 R-117 Ccr-0022 SCA-12 Ccr-0037 GU-228P Ccr-008 GA-11 Ccr-0023 P-7 Ccr-0038 GU-123P Ccr-009 SCA-94 Ccr-0024 SPA-4 Ccr-0039 GU-414E Ccr-0010 Amelionad0 Ccr-0025 R-29 Ccr-0040 CC-44 Ccr-0011 SCA-6 Ccr-0026 SIAL Ccr-0041 SPA-9 Ccr-0012 SIC-12 Ccr-0027 UF-242 Ccr-0042 SCA-49 Ccr-0013 NA-36 Ccr-0028 UF-613 Ccr-0043 C-7 Ccr-0014 ICS-6 Ccr-0029 Forastero Ccr-0044 UF-667 Ccr-0015 EET-12 Ccr-0030 A-246 Ccr-0045 THS-64
  • 8. Cacao clones to be acquired this year 2013 100 clones of cacao to be acquired from the International Cocoa Quarantine Center
  • 9. UF18 M01 K2 TS03 Cr-01 PBC 123
  • 10. B. Characterization using morphological markers
  • 11. C. Characterization using molecular markers
  • 12. D. Conduct Genetic Diversity Analysis as basis for Hybrid Breeding 1. Morphological analysis 2. Isozyme analysis 3. Molecular marker analysis
  • 13.
  • 14. A. Breeding of Desirable Cacao Germplasm a. Selection of parents b. Emasculation/hybridi zation c. Evaluation of hybrids d. Selection of desirable hybrid plant e. Clonal evaluation
  • 15.
  • 16.
  • 17. Crosses Made: 1. BR 25 x K2 8. P 1 x P8 2. BR 25 x K1 9. UF 18 x BR 25 3. BR 25 x S5 10. UF x K1 4. K2 x K1 11. UF x K2 5. K1 x K2 12. UF x S5 6. BR 25 x P1 13. UF x PBC 123 7. BR 25 x P8 14. S5x K2
  • 18. BR 25 X K2
  • 19. BR 25 X K2
  • 20. BR 25 X K1
  • 21.
  • 22. PARTICIPATING COUNTRIES  USM, Philippines  MCB, Malaysia  ICCRI, Indonesia  CCI, Papua New Guinea  NLU, Vietnam  CPCRI, India
  • 23. Hybrids harvested this 2nd of November 2012.
  • 24.
  • 25. Table 1. Kind crosses, no. of flowers pollinated, no. of fruits developed and percentage (%) success pollination of cacao. CROSSES No. of flowers No. of fruits Percentage of success Female Male pollinated developed pollination (%) UF 18 BR 25 186 10 5.4 UF 18 K2 206 45 21.8 UF 18 K1 152 31 20.3 UF 18 PBC 123 188 30 15.9 UF 18 S5 154 8 5.2 S5 K2 20 5 25 K9 S5 30 7 26.5 TOTAL 936 136
  • 26. Philippine cacao hybrid seedlings transplanted in the field (April 2012)
  • 28. Papua New Guinea Hybrids Indonesian Hybrids
  • 29. PNG Cacao Hybrid Trees KEE 42 X K 82 KEE 42 X K72-124 KEE 42 X K 82 KEE 42 X K 82
  • 30. MALAYSIAN HYBRIDS PBC 123 x LAFI 7 PBC 123 x LAFI 7 QH22 x NA 33
  • 31. Table 2. Number of plants, no. of plants with fruits, stem girth and jorquette height of two hybrids from PNG and one hybrid from Malaysia planted in 2009 at USM, Kabacan, Cotabato. No. of No. of plants Stem Girth Jorquette Crosses plants with (cm) Height (cm) fruits Female Male 1. KEE 42 K72-124 30 5 14.2 114.9 2. KEE 42 K82 34 4 16.0 119.0 3. MCBC 3 KKM 22 1 1 19.4 137.0 KEE 42 X K72- 124 KEE 42 X K72- 124 KEE 42 X K72- 124
  • 32. Table 3. Dry bean data of cacao crosses coming from Malaysia and PNG. Crosses Length of Width of Thickness Weight of bean bean (cm) 100 dry Malaysia (cm) (cm) beans (g) QH 22 X NA 33 24.6 1.3 0.8 105.9 PBC 123 X QH 22 25.0 1.3 1.0 127.2 PBC 123 X LAFI 7 24.6 1.3 1.0 130.6 CCI, Papua New Guinea KEE 42 x K72-124 26.6 14.0 1.0 127.1 KEE 42 x K 82 24.0 13.4 0.8 120.4 Control BR 25 23.0 1.3 0 .9 106.3 UF-18 26.0 1.4 0. 9 141.3
  • 33. Three cacao crosses from Malaysia QH 22 X NA 33 PBC 123 X QH22
  • 34.
  • 35.
  • 36. Cocoa Pod Borer (Conopomorpha cramerella) Life Cycle (3‐5 weeks) Adult(3‐7 days) Pupa (5‐7 days) Egg (3‐7 days) Emerging Larva Larva (14‐18 days)
  • 37.
  • 38. 1. Development of Artificial Diet for Cocoa Pod Borer Video file (double click)
  • 39. 2.Vascular Streak Die Back and Pod Rot Studies
  • 40. VSD
  • 41.
  • 42. 3. Research at the nursery level • Effect of different size of polybag on the growth of UF18 cacao seedling • Effect of different media combination on the growth of UF18 cacao seedling • Study on different propagation methods
  • 43. 4. Intercropping Cacao under 20-year old rubber plantation.
  • 44. 5. Determination of Theobromine Content, Butterfat content, etc.
  • 45.
  • 46.
  • 47. Sample of cacao pods of NSIC approved varieties S5 K2 K1
  • 48.
  • 49.
  • 50.
  • 51.
  • 52. 1. Establishment of Budwood Garden in Strategic Location in the Philippines • USM, Kabacan, Cotabato • Naga City • Sta. Maria, Davao del Sur • Lambunao, Iloilo City • San Isidro, Davao del Norte • Coner, Apayao • Mati, Davao Oriental • Sorsogon • Ninoy Aquino, Sultan Kudarat • Zamboanga City • Zamboanga Sibugay • Dipolog, Zamboanga del Norte • Brookes Point, Palawan • Puerto Princesa, Palawan
  • 53.
  • 54. 2. Survey, Identification and Tagging of Existing Budwood Garden
  • 55.
  • 56. Summary • 125 accessions maintained in our genebank • Breeding of cacao and others studies in going-on. • Exchange of hybrids with 5 other countries Malaysia, Indonesia, PNG, Vietnam,India . 10 NSIC approved varieties of cacao in the Philippines .
  • 57. SUMMARY • 3 more new varieties indorse by the NSIC secretariate yesterday to the seed council. PBC 123, USM CH 01, USM CH 02. . Seedling Available for Sale in Cacao Nurseries all over Mindanao are mostly UF 18, BR 25, K2, K9 ande K1.
  • 58. Pure Tablea. Chokolate de Universidad. PICRI, USM,