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Gene Editing
What is CRISPR?
Kevin M. Folta
Professor and Chairman
Horticultural Sciences Department
kfolta.blogspot.com
@...
Research
Funding and Reimbursement: www.kevinfolta.com/transparency
Slides: www.slideshare.net/kevinfolta
What is our starting point?
10
potato
apple
Mechanisms of Genetic Improvement
Central Dogma of Molecular Biology
DNA – genetic material, Hard
copy safe in the nucleus of a cell
RNA – Transient copy of...
Regeneration from single cells
Plant cells can “re-program”
Transgenics
What people usually think of as “GMO”
Addition of a gene, or small number of genes
New information on the hard...
Transgenics
Can add traits from across species (like the Bt gene for insect resistance)
Can suppress traits or viruses usi...
Cisgenics/Intragenics
Transfer of specific
genes from the same
species
Cis-genic = as-is
Intra-genic = all
‘native’ sequen...
Gene Editing = Genome Editing
(changing a single letter in a single document on the
hard drive)
The Gene Editing Revolution
The Gene Editing Revolution
Products of Genetic Engineering
Add a Transgene
ON/OFF GENE
Natural Mutations
ACGAGTCGATCAGTCTAG
Gene Editing
ACGAGTCGATCA...
Applications
Gene Editing
Horn Gene Horn Gene
NO HORNS!!!
Good beef
Bad milkHORNS!!!
Bad beef
Great milk
Cross….
Mix of bad beef, bad m...
Gene Editing
Horn Gene Horn Gene
NO HORNS!!!
Good beef
Bad milkHORNS!!!
Bad beef
Great milk
Horn Gene
NO HORNS!!!
Bad beef...
Gene Editing
Changing
domestic pigs’
DNA to provide
resistance to
African Swine
Fever
Episode 037
X
Farmers
Consumers
Environment
Needy
Low Acrylamide, non Browning Potatoes
X
X
Farmers
Consumers
Environment
Needy
X
Now with Late Blight
resistance and better...
Stopping Citrus Greening
Several genes show
promise
Earliest deregulation is
2019
X
Farmers
Consumers
Environment
Needy
X
X
Health Implications
Health Implications
Health Implications
Universal donor cells to
attack certain leukemias
have been developed using
gene editing to delete gen...
Technical Explosion
Technical Explosion
Technical Explosion
Sweden will not regulate crops produced with gene
editing
USDA appears to be in a similar situation
Ac...
PUBLIC COMMENT PERIOD.
Share your stories to support the development of
gene editing tools and ensure low barriers to
comm...
kfolta.blogspot.com
@kevinfolta
kfolta@ufl.edu
www.talkingbiotechpodcast.com
iTunes, Stitcher, Player FM
Thank you.
All fu...
“Don’t tell me it can’t be done,
tell me what needs to be done
and help me do it.”
Gene Editing in Ag and Medicine
Gene Editing in Ag and Medicine
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Gene Editing in Ag and Medicine

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Presented to the Florida Farm Bureau winter conference, February 7, 2017 in Ocala, FL. The presentation covers the use of new gene editing techniques in animals, plants and medical application. The techniques are contrasted against conventional transgenic approaches. Regulatory concerns were stressed as was the need for ag producers to step up and into the conversation.

Publié dans : Sciences
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Gene Editing in Ag and Medicine

  1. 1. Gene Editing What is CRISPR? Kevin M. Folta Professor and Chairman Horticultural Sciences Department kfolta.blogspot.com @kevinfolta kfolta@ufl.edu www.talkingbiotechpodcast.com
  2. 2. Research Funding and Reimbursement: www.kevinfolta.com/transparency Slides: www.slideshare.net/kevinfolta
  3. 3. What is our starting point? 10 potato apple
  4. 4. Mechanisms of Genetic Improvement
  5. 5. Central Dogma of Molecular Biology DNA – genetic material, Hard copy safe in the nucleus of a cell RNA – Transient copy of the same information (+/-) Protein – Does the work! Enzymes, structures, etc.
  6. 6. Regeneration from single cells Plant cells can “re-program”
  7. 7. Transgenics What people usually think of as “GMO” Addition of a gene, or small number of genes New information on the hard drive!
  8. 8. Transgenics Can add traits from across species (like the Bt gene for insect resistance) Can suppress traits or viruses using RNAi (as in the papaya and potato)
  9. 9. Cisgenics/Intragenics Transfer of specific genes from the same species Cis-genic = as-is Intra-genic = all ‘native’ sequence with some re- arrangement
  10. 10. Gene Editing = Genome Editing (changing a single letter in a single document on the hard drive)
  11. 11. The Gene Editing Revolution
  12. 12. The Gene Editing Revolution
  13. 13. Products of Genetic Engineering Add a Transgene ON/OFF GENE Natural Mutations ACGAGTCGATCAGTCTAG Gene Editing ACGAGTCGATCAGTCTAG ACGAGTCGATGAGTCTAG ACGAGTCGATGAGTCTAG =
  14. 14. Applications
  15. 15. Gene Editing Horn Gene Horn Gene NO HORNS!!! Good beef Bad milkHORNS!!! Bad beef Great milk Cross…. Mix of bad beef, bad milk production
  16. 16. Gene Editing Horn Gene Horn Gene NO HORNS!!! Good beef Bad milkHORNS!!! Bad beef Great milk Horn Gene NO HORNS!!! Bad beef Good milk
  17. 17. Gene Editing
  18. 18. Changing domestic pigs’ DNA to provide resistance to African Swine Fever Episode 037
  19. 19. X Farmers Consumers Environment Needy
  20. 20. Low Acrylamide, non Browning Potatoes X X Farmers Consumers Environment Needy X Now with Late Blight resistance and better cold storage
  21. 21. Stopping Citrus Greening Several genes show promise Earliest deregulation is 2019 X Farmers Consumers Environment Needy X X
  22. 22. Health Implications
  23. 23. Health Implications
  24. 24. Health Implications Universal donor cells to attack certain leukemias have been developed using gene editing to delete genes that lead to the treatment to be rejected from the body.
  25. 25. Technical Explosion
  26. 26. Technical Explosion
  27. 27. Technical Explosion Sweden will not regulate crops produced with gene editing USDA appears to be in a similar situation Activist groups claim that this is an insidious use of technology– wanted it labeled as “GMO” China invested $4 Billion in this technology
  28. 28. PUBLIC COMMENT PERIOD. Share your stories to support the development of gene editing tools and ensure low barriers to commercialize improved genetics! It is critical to be part of this discussion! https://www.regulations.gov/document?D=FDA-2016-N
  29. 29. kfolta.blogspot.com @kevinfolta kfolta@ufl.edu www.talkingbiotechpodcast.com iTunes, Stitcher, Player FM Thank you. All funding, reimbursement: www.kevinfolta.com/transparency All slides: www.slideshare.net/kevinfolta
  30. 30. “Don’t tell me it can’t be done, tell me what needs to be done and help me do it.”

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