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endorfinin (31 amino asit kalnts ieren bir peptit) hidrolizi aadaki amino asitleri retir:
Tyr(1), Giy(3), Phe(2), Met(1), Thr(3), Ser(2), Lys(5), Gln(2), Pro(1), Leu(2), Val(2), Asn(2),
Ala(2), Ile(1), His(1) ve Glu ( 1).
I Karboksipeptidaz ile tedavi Gln'yi serbest brakr.
II DNFB ile tedavi, DNP-Tyr'i serbest brakr.
III Tripsin ile tedavi, aadaki peptitleri verir:
Lys
Gly-Gln
Asn-Ala-Ile-Val-Lys
Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys
Asn-Ala-His-Lys
Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe-Lys
Kimotripsin ile IV Tedavi aadaki peptitleri retir
Lys-Asn-Ala-Ile-Val-Lys-Asn-Ala-His-Lys-Lys-Gly-Gln
-Gly-Gly-Phe
Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe

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endorfinin 31 amino asit kalnts ieren bir peptit hidroliz.pdf

  • 1. endorfinin (31 amino asit kalnts ieren bir peptit) hidrolizi aadaki amino asitleri retir: Tyr(1), Giy(3), Phe(2), Met(1), Thr(3), Ser(2), Lys(5), Gln(2), Pro(1), Leu(2), Val(2), Asn(2), Ala(2), Ile(1), His(1) ve Glu ( 1). I Karboksipeptidaz ile tedavi Gln'yi serbest brakr. II DNFB ile tedavi, DNP-Tyr'i serbest brakr. III Tripsin ile tedavi, aadaki peptitleri verir: Lys Gly-Gln Asn-Ala-Ile-Val-Lys Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys Asn-Ala-His-Lys Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe-Lys Kimotripsin ile IV Tedavi aadaki peptitleri retir Lys-Asn-Ala-Ile-Val-Lys-Asn-Ala-His-Lys-Lys-Gly-Gln -Gly-Gly-Phe Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe