Tape stripping - Three potential alternatives for cosmetic science

Notes de l'éditeur

2. Thank you to IFSCC for giving me the possibility to present my work and thank you CHAIRMAN introducing me. -> Detail what we do
3. Skin science: Penetration (actif), Skin structure, properties Skin from killed pigs
4. Skin science: Penetration (actif), Skin structure, properties Skin from killed pigs
5. Skin science: Penetration (actif), Skin structure, properties Skin from killed pigs
6. Skin science: Penetration (actif), Skin structure, properties Skin from killed pigs
7. Skin science: Penetration (actif), Skin structure, properties Skin from killed pigs
8. Skin science: Penetration (actif), Skin structure, properties Skin from killed pigs
9. Skin science: Penetration (actif), Skin structure, properties Skin from killed pigs
10. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
11. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
12. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
13. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
14. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
15. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
16. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
17. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
18. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
19. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
20. TS one very common method - showing two alternative methods (trypsin, cyano) and discuss their pros and cons-
21. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron Analytical methods: Histological imaging and by CRM - Why CRM? Will be detailed but I can betray that it serves also as an alternative. -> CRM
22. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron Analytical methods: Histological imaging and by CRM - Why CRM? Will be detailed but I can betray that it serves also as an alternative. -> CRM
23. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron Analytical methods: Histological imaging and by CRM - Why CRM? Will be detailed but I can betray that it serves also as an alternative. -> CRM
24. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron Analytical methods: Histological imaging and by CRM - Why CRM? Will be detailed but I can betray that it serves also as an alternative. -> CRM
25. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron Analytical methods: Histological imaging and by CRM - Why CRM? Will be detailed but I can betray that it serves also as an alternative. -> CRM
26. Why also CRM? How does it work also an alternative and other advantages
27. Advantages of CRM -> Technique 1. SC thickness
28. Advantages of CRM -> Technique 1. SC thickness
29. Advantages of CRM -> Technique 1. SC thickness
30. Advantages of CRM -> Technique 1. SC thickness
31. Advantages of CRM -> Technique 1. SC thickness
32. Advantages of CRM -> Technique 1. SC thickness
33. Advantages of CRM -> Technique 1. SC thickness
34. Advantages of CRM -> Technique 1. SC thickness
35. Advantages of CRM -> Technique 1. SC thickness
36. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
37. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
38. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
39. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
40. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
41. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
42. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
43. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
44. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
45. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
46. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
47. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
48. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
49. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
50. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
51. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
52. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
53. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
54. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
55. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
56. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
57. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
58. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
59. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
60. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
61. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
62. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
63. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
64. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
65. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
66. Baseline Linear von Punkt 2500 bis 3900 2931 ν(CH3) asymmetric stretching vibration 3450 ν(OH) stretching vibration 3329 ν(NH) Vibration of protein 2910-2950 Integral Protein and 3350-3550 Water ν = stretch 2852 ν(CH2) symmetric 2883 ν(CH2) asymmetric Measured at 0µm and ~6µm -> H2O vs Depth
67. Different methods of SC limit Interpretation steep slope -> Practical; Tape Stripping
68. Different methods of SC limit Interpretation steep slope -> Practical; Tape Stripping
69. Different methods of SC limit Interpretation steep slope -> Practical; Tape Stripping
70. Different methods of SC limit Interpretation steep slope -> Practical; Tape Stripping
71. Different methods of SC limit Interpretation steep slope -> Practical; Tape Stripping
72. D-Squame® adhesive tapes (diameter 22 mm, surface 3.8 cm2, Monaderm, Monaco) for 10 seconds using a 500 g mass stick -> Histological Image
73. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
74. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
75. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
76. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
77. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
78. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
79. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
80. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
81. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
82. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
83. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
84. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
85. Hematoxylin–Phloxin–Saffron: Blue: Cell nuclei by hematoxylin; Pink: cytoplasm by phloxine; Orange/Yellow: extracellular matrix of connective tissue by saffron 9 strips: 50% 28 strips 20% remains -> CRM
86. before : 10µm after: irregulaire
87. before : 10µm after: irregulaire
91. 0.0016 wt-% trypsin (Sigma-Aldrich) in PBS pH 7.2 for 2-3 hours @ 40°C Stop reaction with a trypsin inhibitor is possible. SC separated surgical blade no. 11 after rinsing off skin surface with phosphate buffered saline (PBS) buffer -> Histological Image
94. Before: 7.33 ± 0.67 After: insignificative
95. Before: 7.33 ± 0.67 After: insignificative
98. microscope slide (76 x 26 mm; Roth, Karlsruhe, Germany) coated w cyanoacrylate resin (Loctite Super Glue-3 from Henkel France) applied on SC for defined polymerization times: 3 x 2min and 2 x 15 min Removing slide detached SC [64]. -> Histological Image
99. 3 times 2 minutes
100. 3 times 2 minutes
101. 3 times 2 minutes Before: 7.5±0.5 µm After: 5±0.58µm -> 2 times 15 min Histological Image
102. 3 times 2 minutes Before: 7.5±0.5 µm After: 5±0.58µm -> 2 times 15 min Histological Image
103. 2 times 15 minutes
104. 2 times 15 minutes
105. 3 times 2 minutes Before: 10 ± 0.82 µm After: 1±1µm -> Resume via direct comparison of Histological Image
106. 3 times 2 minutes Before: 10 ± 0.82 µm After: 1±1µm -> Resume via direct comparison of Histological Image
107. -> Resume via table
108. -> Resume via table
109. -> Let me conclude the results of this study
110. -> Let me conclude the results of this study
111. -> Let me conclude the results of this study
113. Conclusion: Cyano and Trypsin are best, but CRM seems to has potential -> Detail with data:
114. Conclusion: Cyano and Trypsin are best, but CRM seems to has potential -> Detail with data:
115. Conclusion: Cyano and Trypsin are best, but CRM seems to has potential -> Detail with data:
116. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
117. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
118. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
119. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
120. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
121. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
122. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
123. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
124. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
125. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
126. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
127. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
128. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
129. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
130. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
131. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
132. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
133. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
134. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
135. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
136. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
137. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
138. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
139. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
140. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
141. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
142. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
143. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
144. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
145. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
146. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
147. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
148. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
149. For opening discussion! TS In vivo: minimal invasive CRM Standardized: characteristical peak for actives etc. furrows better with cyano than ts and also glands ->Let me repeat the conclusion
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180. CRM is a innovative, new method and has to be developed a lot more during the next years -> Discussion
181. CRM is a innovative, new method and has to be developed a lot more during the next years -> Discussion
182. It will be a pleasure answering any question concerning these methods and invite you for an expierience exchange