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BT-202 Netaji Subhas Institute of Technology, Dwarka, New Delhi. Dr. Amita Pandey Aug 24, 2011
Syllabus for mid-term Properties of water  ✔ Acids and bases and buffers  ✔ Covalent bond ✔ Non-covalent interactions in biological system  ✔ Carbohydrates  ✔ Proteins Enzymes Lipids Nucleic acids Vitamins and Co-enzymes Separation technique for biomolecules
Learning check! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Polysaccharides ,[object Object],[object Object],[object Object],[object Object],[object Object]
 
[object Object],[object Object],[object Object],Homopolysaccharides as stored fuel
Starch and Glycogen
Starch ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],Glycogen
Homoploysaccharides as structural biomolecules ,[object Object],[object Object],[object Object]
Cellulose ,[object Object],[object Object],[object Object]
Chitin ,[object Object]
Heteropolysaccharides as structural components ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
Polysaccharides as information carriers ,[object Object],[object Object],[object Object],[object Object]
Glycoconjugates ,[object Object],[object Object],[object Object]
Glycoproteins ,[object Object]
Glycoproteins ,[object Object],Oligosaccharide chains are attached either posttranslation or cotranslation modification.
[object Object],[object Object]
[object Object],[object Object],[object Object]
Separation techniques for Carbohydrates
Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object]
Gel Filteration ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Affinity chromatography ,[object Object],[object Object],[object Object]
Affinity Chromatography ,[object Object],[object Object]
High Performance Liquid Chromatography (HPLC) ,[object Object],[object Object],[object Object]
Mass Spectrometery ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Oligosaccharides in a group of glycoproteins analyzed  by MALDI MS
Learning check! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
Amino Acids, Peptides, and Proteins
Amino acids ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Zwitterions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Zwitterions ,[object Object],[object Object]
Classification of Amino Acids ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Non-polar, aliphatic R groups
Polar, uncharged R group
Disulfide Bonds
Aromatic R groups
UV-Vis Spectroscopy ,[object Object]
Acidic and Basic Amino Acids ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Uncommon amino acids ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Acid-base Properties ,[object Object],pK 1  ~ 2.2 (protonated below 2.2) pK 2  ~ 9.4 (NH 3 +  below 9.4) pK R (when applicable)
3-letter and 1-letter abbreviations
pH and Ionization
Titration of Glycine ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Titration curve of histidine -Aas with ionizable R groups have three pKa  values
pI  ,[object Object],[object Object],[object Object],[object Object],[object Object]
Stereochemistry of AAs ,[object Object],[object Object]
D and L Configurations  ,[object Object],[object Object],[object Object]
Importance of Stereochemistry ,[object Object],[object Object],[object Object],[object Object],[object Object]
Learning Check! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
The Peptide Bond
Polypeptides ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Naming  Peptides ,[object Object],[object Object],[object Object]
Learning Check! Ser-Gly-Tyr-Ala-Leu serylglycyltyrosylalanylleucine
Calculate number of AAs present in a protein ,[object Object],[object Object]
Separation and purification ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Concentration of salts ,[object Object],[object Object],[object Object],[object Object]
Types of Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Types of Chromatography ,[object Object],[object Object],[object Object]
Electrophoresis Separation of proteins based on their charge in an electric field.
[object Object],[object Object],[object Object],[object Object]
Isoelectric focusing ,[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
Agar ,[object Object],[object Object],[object Object]
 
 
Ion Exchange Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Ion Exchange Chromatography
Affinity Chromatography

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