Ce diaporama a bien été signalé.
Nous utilisons votre profil LinkedIn et vos données d’activité pour vous proposer des publicités personnalisées et pertinentes. Vous pouvez changer vos préférences de publicités à tout moment.

Medical mycology

8 449 vues

Publié le

Medical Mycology

Publié dans : Santé
  • I have suffered from a widening array of allergies and constant cravings for sugar and bread. I was miserable most of the time while taking allergy medication. After following your step-by-step directions for 6 weeks, I am recovering! I am now, as I write this lines, almost symptom free. I recommend anyone who is affected by allergy caused directly or indirectly by candida yeast infection to read your book. It is so easy to read (even for me - English is not my native language) and to apply. I cannot thank you enough for your counseling service and for the knowledge and health you have brought into my life. ●●● https://tinyurl.com/y4uu6uch
       Répondre 
    Voulez-vous vraiment ?  Oui  Non
    Votre message apparaîtra ici
  • Six weeks into the program and I no longer suffer from the debilitating symptoms that had practically ruined my life. There are no more migraines, no more joint and knee pains, no more menstrual pain, no more rashes on my chest and no more seborrhea on my eyebrows. It is truly a miracle that you have given me...  http://scamcb.com/index7/pdf
       Répondre 
    Voulez-vous vraiment ?  Oui  Non
    Votre message apparaîtra ici
  • Six weeks into the program and I no longer suffer from the debilitating symptoms that had practically ruined my life. There are no more migraines, no more joint and knee pains, no more menstrual pain, no more rashes on my chest and no more seborrhea on my eyebrows. It is truly a miracle that you have given me...  http://scamcb.com/index7/pdf
       Répondre 
    Voulez-vous vraiment ?  Oui  Non
    Votre message apparaîtra ici
  • Hello! High Quality And Affordable Essays For You. Starting at $4.99 per page - Check our website! https://vk.cc/82gJD2
       Répondre 
    Voulez-vous vraiment ?  Oui  Non
    Votre message apparaîtra ici
  • very interesting
       Répondre 
    Voulez-vous vraiment ?  Oui  Non
    Votre message apparaîtra ici

Medical mycology

  1. 1. Classification, Lab diagnosis and Important diseases caused by Fungi 2/13/2015 1 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  2. 2. Objectives To know about important characteristics of fungi To know various classification systems To know medically important fungi To know the lab diagnosis of fungi To know treatment of fungal diseases 2/13/2015 2 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  3. 3. Mycology • Study of fungi – mycology • Mykos – mushroom • All fungi are eukaryotic • Natural habitat- soil, water and decaying organic debris • Obligate or facultative aerobe • Chemotrophic organisms 2/13/2015 3 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  4. 4. • Differences from bacteria – Posses rigid cell wall – Contain chitin, mannan and polysacharide – Cytopalsmic membrane contain sterols – True nuclei with Nuclear membrane, mitochondria and endopalsmic reticulum – Unicellular or multicellular – Divide by asexually, sexually or by both 2/13/2015 4 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  5. 5. Classification A. Taxanomic classification  Phyllum Thallophyta  Four calsses of fungi B. Morphological classification  Yeasts  Yeasts like fungi  Moulds  Dimorphic fungi 2/13/2015 5 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  6. 6. Thallophyta Thallophyta Irregular plant masses lacking definite root, stem and leaf structures Fungi Algae (No chlorophyll) (Chlorophyll) 4 classes Zygomycetes Ascomycetes Basidiomycetes Deuteromycetes or fungi imperfecti • Lower fungi having non-septate hyphae • Forms asexual spores sporangiospores • Sexual spores zygospores and oospres • Septate hyphae • Sexual spores (ascospores) are present within the sac or ascus • Septate hyphae •Sexual spores are basidiospores on a basidium • Septate hyphae •Lack a known sexual state. Most fungi medically important fungi belongs to this class 2/13/2015 6 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  7. 7. Yeasts i. Round to oval unicelluar ii. Reproduce by budding iii. Creamy mucoid colonies iv. E.g. Cryptococcus neoformans 2/13/2015 7 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  8. 8. Yeasts like fungi • Partly as yeasts and partly as elongated budding cells • Germ tube to demonstrate pseudohyphae 2/13/2015 8 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  9. 9. Moulds 1. Grow as branching filaments – hyphae 2. Hyphae septate or nonseptate 3. Continue growth called as mycelium 4. E.g Dermatophytes, Aspergillus, Penicillium and Rhizophus 2/13/2015 9 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  10. 10. Dimorphic fungi • They exists as yeasts in host tissue and in cultures as mycelial growth • Blastomyces dermatitidis, Paracoccidioides, Coccidiodes immitis, Histoplasma, Sporothrix 2/13/2015 10 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  11. 11. Reproduction and sporulation • Sexual spores – oospres, ascospores, zygospores and basidiospores • Vegetative spores – – Blastospores:formed by budding from parent cell – Arthrospores: cross septa into hyphae • Aerial spores: Conidiospores, Microconidia, Macroconidia, Sporangiospores 2/13/2015 11 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  12. 12. Laboratory Diagnosis A. Direct microscopy – KOH preparation – Gram staining – India ink preparation B. Culture - SDA C. Slide culture test 2/13/2015 12 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  13. 13. Classification of fungal diseases A. Superficial mycoses- skin nails hair B. Subcutaneous mycoses – myetoma C. Systemic mycoses - aspergillosis 2/13/2015 13 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  14. 14. Dermatophytes • They infect superficial keratinized tissue (skin, nail and hair) without involving tissue • They break down and utilizes keratin • Incapabale of penetrating subcutaneous tissue • Cause dermatomycoses also known as ringworm 2/13/2015 14 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  15. 15. Cylindrical macroconidia Fusiform macroconidia Club shaped macroconidia 2/13/2015 15 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  16. 16. 2/13/2015 16 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  17. 17. Classification – Trichophyton - hair, skin and nail – Microsporum – hair , skin – Epidermophyton – skin and nail  Lab diagnosis:  Specimen: skin, hair or nail  Direct microscopy – LPCB mount  Culture  Treatment 2/13/2015 17 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  18. 18. Subcutaneous Mycoses • Mycetoma : crhonic granulomatous infection of subcutaneous tissue usually affects foot • Also known as Madura foot • Mainly in tropical countirs • Common in Tamilnadu • Caused by Actinomycetes and filamentous fungi • Enter through minor trauma • Diagnosis made by observing granules • Treatment- sulphonamides sometime amputation 2/13/2015 18 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  19. 19. Sporotrichosis • Is nodular, ulcerating disease of skin and subcutaneous tissue • Acces through thorn pricks or injuries • Spread through lymphatics upto regional lymph nodes • Sporothrix schenckii – dimorphic fungi • Lab diagnosis by culture 2/13/2015 19 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  20. 20. Rhinosporidiosis • Is a chronic granulomatous disease characterized by formation of friable polyps, usually confined to nose, mouth or eye • Causative agent Rhinosporidium seeberi • Reported from Srilanka and India • Mode of infection is not known • Lab diagnosis done by demonstration of sporangia 2/13/2015 20 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  21. 21. Rhinosporidiosis 2/13/2015 21 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  22. 22. Systemic mycoses 1. Histoplasmosis – Histoplasma capsulatum – Reticuloendothelial system – Source – soil with excreta of birds – Asymptomatic – Sputum, bone marrow, blood, scrapings, biopsies – Geimsa stain or Wright stain – Culture- SDA – Amphotericin B 2/13/2015 22 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  23. 23. 2. Blastomycosis – Blastomyces dermatitidis- dimorphic fungi – Infection mainly to skin, bone and genitourinary tract – Inhalation of conidia – Asymptomatic – Sputum, pus or scrapings – 10% KOH, H&E stain and PAS stain – Culture-SDA – Amphotericin B 2/13/2015 23 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  24. 24. 3. Paracoccidiomycosis – Paracoccidioides brasilensis- dimorphic fungi – Inhalation of spores – Same 4. Coccidiodomycosis 5. Crptococcosis - Crptococcus neoformans - Inhalation of dust - Seen in immunocompromised - demosntration of capsule by india ink 6. Opportunistic mycoses- candiasis, aspergillosis, zygomycoses 2/13/2015 24 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  25. 25. Aspergillus fumigatus 2/13/2015 25 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  26. 26. Aspergillus terreus 2/13/2015 26 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  27. 27. Aspergillus fumigatus 2/13/2015 27 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  28. 28. ASpergillus niger 2/13/2015 28 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  29. 29. Typical growth of Aspergillus spp 2/13/2015 29 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  30. 30. ASpergillus flavus 2/13/2015 30 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  31. 31. Mucor 2/13/2015 31 Mohammad Mukhit Kazi, Lecturer SDCH Pune
  32. 32. Take home message • Fungi are eukaryotes • Medically important fungi belongs to Fungi imperfecti or Deuteromycetes • Sabourauds dextrose agar is used to culture • Slide culture to demonstrate better morphological characters • KOH mount or LPCB mount for demonstration • Mainly opportunistic infections • E.g. Candidiasis, Cryptococcosis, Aspergillosis 2/13/2015 32 Mohammad Mukhit Kazi, Lecturer SDCH Pune

×