1. DIAGONOSIS OF MYCOBACTERIUM
TUBERCULOSIS
BY
MUHAMMAD ZADA

2. Introduction
 Pathogenic bacteria of mycobacteraceae family
 Aerobic bacilli –non spore forming
non motile
 Cell wall –rich in lipids
 Acid-fast bacilli
 Very slow growing
 Route of transmission- aerosole

3.  TB drug resistance: resistant to one or more of first
line drugs: isoniazid, rifampin, pyrazinamide and
ethambutol (~ 5% in Canada)
 MDR-TB: resistance to at least INH and rifampin (~
0.7% in Canada)
 XDR-TB: resistance to all first line therapeutic drugs
(rare)

4. Diagnostic Testing
 Culture is still the recommended method for
diagnosing TB
 Detection of AFB in respiratory secretions
 Chest X-ray
 Tuberculin skin test (TST)
 Molecular diagnostic tests

5. procedure
 Pulmonary or extra pulmonary samples are first
decontaminated (NAL soln.)
 Smear formation
 Inoculation to LJ and MGIT media.

6.  Smear formation

7.  After inoculation of specimen the MGIT are kept in
bactec for 42 days
 If any tube appears positive,
 It should be sub cultured, acid fast stained and
treated as presumptive positive

11.  thank you