Aucune remarque pour cette diapositive
The work I will present today has been focussed on one particular protein, Gadd45b, one of a number of related proteins involved in the response to cellular stress. Upon DNA damage, and various other types of cellular stress, expression of these proteins is augmented. CLICK In the case of the Gadd 45 proteins, these can subsequently induce cell cycle arrest CLICK And these proteins are also invovled directly in DNA repair CLICK and have also been implicated in tumour suppression and epigenetic control of gene expression
In order to confirm this observation , the amount of fibers was objectively quantified in each animal from this study. Each lobe of each mouse was analyzed and approximately 800 images were generated from 40 animals included in 3 independent experiments This slide shows two examples of liver sections from a nontransgenic and a transgenic animal, both treated with CCl4, with, below, the image generated in silico for quantification of fiber deposition. The histogram on the right summarizes the results of this analysis. As you can see, there was no difference between non-transgenic and transgenic animals in the absence of CCl4 treatment. In contrast, in CCL4-treated animals, fibrosis was significantly more abundant in the liver of transgenic than non-transgenic animals, objectively confirming the histological observation.