5. Specimen Rejection Criteria:
• Improperly labeled or unlabeled
• Improperly collected or preserved
• Submitted without properly completed request form
• Contaminated form
• Improperly volume & leakage sample
6. Patient’s specimen:
• A properly labelled sample is essential so that the results of the test
should match the patient.
• Label with patient’s ID or the sample number provided for the
7. Sites of Blood Collection
Blood can be collected from 3 different sources:
• Capillary blood.
• Venous blood.
• Arterial blood.
8. Capillary Blood Collection
Method of Collection :
• Select the least used finger.
• Cleanse the site with alcohol swab.
• Puncture across the grain of the skin
• And transfer blood to a strip or small container.
• To draw a small amount of blood
• In a microtube or strip for blood sugar and
• Bleeding time tests.
• For infants and young children.
12. Venous Blood Collection
• Equipment required
• Vacutainer and syringe
• Alcohol swab
• Majority of routine tests are performed on venous blood.
• Blood can be taken directly from the vein.
• The best site - deep veins of the ante-cubital fossa.
• Fore arm vein:
• Other sites are: Dorsum of hand:
• Femoral vein:
• Jugular vein
• Scalp vein
21. - Fasting blood Sample
- Postprandial blood sample
Approximately 8 to12 hours fast before blood
-Drink only water
- Regular drug allow
2 Hours after Regular major Meal
22. Method Of Blood Collection
1. With Syringe and non-vacuum Vacutainer
2. With vacuum Vacutainer
3. With Butterfly Needle
23. 1) With Syringe and Non-vacuum Vacuette
• Not extra accessory require
• Improper volume – especially when blood
collection is require for coagulation profile
24. 2) With Vacuum Vaccutiner
• Maintain proper volume of blood
• Safe & Speedy
• Reducing the risk of haemolysis
• not suited for small veins.
25. 3) With Butterfly Needle
• The risk of hemolysis
• It is difficult to collect large quantity of blood as well as in multiple
• Useful in infants & children
27. Preanalytic Interference in Sample
• Reddish discoloration of serum/plasma due to rupture of RBCs.
• Factor causing haemolysis
• Sampling = Inject forcefully in vacutte
• Store = Frozen = Due to high or low temp,
• Vigorously shaking of blood - trasnporatation
• Yellowish discoloration of Serum due to high bilirubin.
• Milky or Turbid appearance of Serum due to high Triglyceride
29. Top Color Additives Principle Uses
-The strongest anti-coagulant
-Ca+2 chelating agent
- Blood bank
Light Blue Sodium
Ca+2 chelating agent - PT
Heparin binds to Thrombin and
inhibits the second step in the
K+, Mg+, Cl)
Plasma Separating Tubes (PST)
30. Top Color Additives Principle Uses
Gray -Sodium Fluoride
Top Tubes Additives Principle Uses
Sometimes it has gel
or silicon at the
bottom of tube to
Blood cross matching
Serum Separating Tubes (SST)
41. Preservation of Blood
• For many purposes blood may be safely persevered at 4ºC in
• EDTA is best preservative for Hemogram.
• Tri-sodium citrate best for coagulation study.
• Before procedure, the blood should be first allowed to warm up to
room temperature, then mixed, preferably by rotation, for at least 2