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Aim, Principle, Materials, Procedure and Result for Serial Dilution Technique
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Enumeration is counting of microorganisms present in a sample. This is done to know the intense of presence of the spoilers in the spoiled food. To detect which type of organism is responsible for the spoilage. Mostly this is done two important methods. Viable count Total count VIABLE COUNT: A viable cell count allows one to identify the number of actively growing or dividing cells in a sample. The plate count method or spread plate method relies on bacteria growing a colony on a nutrient medium. Number of colonies can be counted. Plate count agar is used for general count MacConkey agar is used for Gram negative organisms. TOTAL COUNT: The initial analysis is done by mixing serial dilution of sample in liquid nutrient agar which is then poured into bottles. The bottles are then sealed and laid on their sides to produce a slopping agar surface. The colonies are then counted by eye.The total number of colonies are said as Total Viable Count. The initial analysis is done by mixing serial dilution of sample in liquid nutrient agar which is then poured into bottles. The bottles are then sealed and laid on their sides to produce a slopping agar surface. The colonies are then counted by eye.The total number of colonies are said as Total Viable Count. Pour plate method: The same procedure is done for this till serial dilution. The serially diluted sample is then mixed with the molten nutrient agar. Then poured onto the sterile petridish. Incubated under appropriate temperature amd the colonies where counted. ConclusionThe enumeration of these spoiled food samples are important to encounter the type of microbe is causing the spoilage. And hence this is used to prevent the same type of spoilage. This can be avoided by making the environmental changes which inhibits the organism which is responsible for the spoilage.
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Serial dilution
1.
Announcement Next test
(Test 2) is on March 22nd, 2011 Tuesday at the big Hall 8pm Test 4 is on April 5th, 2011 Tuesday at the big Hall 8pm
2.
Serial Dilution Pay
attention as I’m going to Quiz you on this TODAY
3.
Serial Dilution
4.
Too much growth,
hard to count colonies Growth not as much, but still hard to count colonies OK to count but there are some colonies that are not single colonies, they are so close together Definitely the best plate to estimate the CFU
5.
Serial Dilution cont’d
6.
Try this!!!!!
7.
Did you get
5.6 x 108???
8.
9.
10.
11.
12.
5.6 x 108
CFU/ml
Download now