Lab bench activity on mitosis and meiosis

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Name:_____________________
Mitosis and Meiosis
Lab-bench activities
Go to: http://www.phschool.com/science/biology_place/labbench/lab3/intro.html
Click on Introduction
1- What organisms are used to study Mitosis?

2- What organism is used for the study of Meiosis?

3- What is the difference between Haploid and diploid?

4Mitosis

Meiosis

# of cell divisions
# of daughter cells
# of Chromosomes

5-What is the cell cycle? Is it present in both cell divisions?

6-What is the longest phase of the cell cycle? What does the cell do during this phase?

7- What are Homologous chromosomes? Explain Sister Chromatids? You may Sketch the Homologous
chromosomes and label the sister Chromatids

2

7- Name the phases of Mitosis

8- What is Cytokinesis?

9- Can you recognize the phases in real tissue?

10- Explain alleles.

11Stage of Mitosis

# of cells in this phase

12- Take the quiz and put your answers below

Duration of the stage=%of cells
in the stage/1440 min.

3
Meiosis
12-How is Prophase I in Meiosis different from Prophase in Mitosis?

13-Name 3 differences you see when comparing Mitosis to Meiosis

14-Explain Synapsis and Crossing over. Sketch a diagram to represent both. Use colored pencils to show
this event.

15-What is the genetic significance of crossing over?

16-What is Sordaria? How does fertilization occur in Sordaria?

17-What is an ascus?

18- What happens after fertilization?

4
19-What would constitute a crossing over in an ascus?

20-Sketch one ascospore without crossing over, and another one with crossing over.

21-Count crossing overs in Experiment II. Were you accurate in your counting?

22-What does the frequency of crossing over allow scientists to do?

23- What is a map unit? How do you go about calculating the map distance between the color gene of
the spores and the centromere in Sordaria?

24- Calculate the map distance using the formula given in this exercise. Show your work.

25- Why do you divide the percentage of crossing over by 2?

26-Compare Mitosis to Meiosis and check your answers. What is the one fact you learned in your
answering the questions?

27- Take the self- quiz and write your answers below.

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Experiment 2: The Importance of Cell Cycle Control Some environmental factors can cause genetic mutations which result in a lack of proper cell cycle control (mitosis). When this happens, the possibility for uncontrolled cell growth occurs. In some instances, uncontrolled growth can lead to tumors, which are often associated with cancer, or other biological diseases. In this experiment, you will review some of the karyotypic differences which can be observed when comparing normal, controlled cell growth and abnormal, uncontrolled cell growth. A karyotype is an image of the complete set of diploid chromosomes in a single cell. Materials *Computer Access *Internet Access *You Must Provide Procedure 1. Begin by constructing a hypothesis to explain what differences you might observe when comparing the karyotypes of human cells which experience normal cell cycle control versus cancerous cells (which experience abnormal, or a lack of, cell cycle control). Record your hypothesis in Post-Lab Question 1. Note: Be sure to include what you expect to observe, and why you think you will observe these features. Think about what you know about cancerous cell growth to help construct this information 2. Go online to find some images of abnormal karyotypes, and normal karyotypes. The best results will come from search terms such as “abnormal karyotype”, “HeLa cells”, “normal karyotype”, “abnormal chromosomes”, etc. Be sure to use dependable resources which have been peer-reviewed 3. Identify at least five abnormalities in the abnormal images. Then, list and draw each image in the Data section at the end of this experiment. Do these abnormalities agree with your original hypothesis? Hint: It may be helpful to count the number of chromosomes, count the number of pairs, compare the sizes of homologous chromosomes, look for any missing or additional genetic markers/flags, etc. 4. Take a picture of your results. Include a note with your name and date on an index card in the picture. Insert picture here. Data 1. 2. 3. 4. 5. Experiment 1: Following Chromosomal DNA Movement through Meiosis In this experiment, you will model the movement of the chromosomes through meiosis I and II to create gametes. Materials 2 Sets of Different Colored Pop-it® Beads (32 of each - these may be any color) 8 5-Holed Pop-it® Beads (used as centromeres) Procedure: Part 1: Modeling Meiosis without Crossing Over As prophase I begins, the replicated chromosomes coil and condense… 1. Build a pair of replicated, homologous chromosomes (Figure 3). 10 beads should be used to create each individual sister chromatid (20 beads per chromosome pair). Two five-holed beads represent each centromere. To do this... Figure 3:Bead set-up. The blue beads represent one pair of sister chromatids and the black beads represent a second pair of sister chromatids. The black and blue pair are homologous. a. Start with 20 beads of the same color to create your first sister chrom ...

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1) Most of the chromosomes in humans are sex chromosomes. True/False 2) The same sequence of genes is found on ______chromosomes 3) In the human life cycle, somatic cells are: a)diploid, while gametes are diploid. b)diploid, while gametes are haploid. c)haploid, while gametes are diploid. d)haploid, while gametes are haploid. 4) If the human reproductive cycle only included diploid cells, the offspring of each generation would have twice as much genetic material in each cell as in the parent cells. true/false 5) If the somatic cells of a species have 25 pairs of homologous chromosomes, the number of chromosomes in haploid gametes would be____ 6) Sister chromatids are separated during mitosis and _____ 7)In what types of organism(s) and where does meiosis occur? What type of cell division occurs in bacteria? 8) How many cells are produced at the end of meiosis? 9) How many chromosomes does each of the daughter cells contain? 10) Are these daughter cells haploid, diploid, or tetraploid? Explain. 2) The same sequence of genes is found on ______chromosomes 3) In the human life cycle, somatic cells are: a)diploid, while gametes are diploid. b)diploid, while gametes are haploid. c)haploid, while gametes are diploid. d)haploid, while gametes are haploid. 4) If the human reproductive cycle only included diploid cells, the offspring of each generation would have twice as much genetic material in each cell as in the parent cells. true/false 5) If the somatic cells of a species have 25 pairs of homologous chromosomes, the number of chromosomes in haploid gametes would be____ 6) Sister chromatids are separated during mitosis and _____ 7)In what types of organism(s) and where does meiosis occur? What type of cell division occurs in bacteria? 8) How many cells are produced at the end of meiosis? 9) How many chromosomes does each of the daughter cells contain? 10) Are these daughter cells haploid, diploid, or tetraploid? Explain..

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Lab Template Week 4: Meiosis Submitted by: <your name here> As you complete the lab, record your answers in this template. Save the document as LastName_FirstName_BIO1020_W4A3, and submit it to the Dropbox. Full lab instructions and the rubric with which you will be evaluated can be found in the online classroom. Activity Cellular reproduction in Eukaryotes involves either mitosis or, in the case of sex cells, meiosis. Mitosis involves the reproduction of a cell into two identical daughter cells. Meiosis, however, is a reduction division where a parental diploid cell produces four haploid gametes. Upon fusion, two haploid gametes (in humans the sperm and the egg) will result in one diploid zygote. In this activity you will track chromosomes through meiosis using colored beads. Experiment 1 Questions 1. Why is crossing over important in heredity? (10 points) 2. Provide two ways that meiosis I and meiosis II are diﬀerent. (10 points) 3. (10 points) a. In the lab, how many chromosomes were present in each cell when meiosis I started? b. How many chromosomes were present in each daughter cell at the end of meiosis II? 4. If humans have 46 chromosomes in each of their body cells, determine how many chromosomes you would expect to find in the following (10 points): Sperm: Egg: Daughter cell from mitosis: Daughter cell from meiosis II: 5. Why is it necessary to reduce the chromosome number of gametes, but not of other cells of an organism? (10 points) 41 Meiosis Lab 4 42 Lab 4: Meiosis 43 Introduc on Meiosis only occurs in organisms that reproduce sexually. The process generates haploid (1n) cells called gametes (sperm cells in males and egg cells in fe- males), or spores in some plants, fungi, and pro sts, that contain one complete set of chromosomes. Haploid cells fuse together during fer liza on to form a diploid cell with two copies of each chromosome (2n). Genes are the units of heredity that have speciÞc loci (loca ons) on the DNA strand and code for inheritable traits (such as hair color). Alleles are alterna ve forms of the same gene (brown vs. blue eyes). Homol- ogous chromosomes contain the same genes as each other but o en di erent alleles. Non-sex cells (e.g. bone, heart, skin, liver) contain two alleles (2n), one from the sperm and the other from the egg. Mitosis and meiosis are similar in many ways. Meiosis, however, has two rounds of division—meiosis I and meiosis II. There is no replica on of the DNA between meiosis I and II. Thus in meiosis, the parent cell produces four daughter cells, each with just a single set of chromosomes (1n). Meiosis I is the reduc on division– the homologous pairs of chromosomes are separated so that each daughter cell will receive just one set of chromosomes. During meiosis II, sister chroma ds are separated (as in mitosis). Concepts to explore: Meiosis Diploid cells Haploid cells ...

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1. 1 Name:_____________________ Mitosis and Meiosis Lab-bench activities Go to: http://www.phschool.com/science/biology_place/labbench/lab3/intro.html Click on Introduction 1- What organisms are used to study Mitosis? 2- What organism is used for the study of Meiosis? 3- What is the difference between Haploid and diploid? 4Mitosis Meiosis # of cell divisions # of daughter cells # of Chromosomes 5-What is the cell cycle? Is it present in both cell divisions? 6-What is the longest phase of the cell cycle? What does the cell do during this phase? 7- What are Homologous chromosomes? Explain Sister Chromatids? You may Sketch the Homologous chromosomes and label the sister Chromatids

2. 2 7- Name the phases of Mitosis 8- What is Cytokinesis? 9- Can you recognize the phases in real tissue? 10- Explain alleles. 11Stage of Mitosis # of cells in this phase 12- Take the quiz and put your answers below Duration of the stage=%of cells in the stage/1440 min.

3. 3 Meiosis 12-How is Prophase I in Meiosis different from Prophase in Mitosis? 13-Name 3 differences you see when comparing Mitosis to Meiosis 14-Explain Synapsis and Crossing over. Sketch a diagram to represent both. Use colored pencils to show this event. 15-What is the genetic significance of crossing over? 16-What is Sordaria? How does fertilization occur in Sordaria? 17-What is an ascus? 18- What happens after fertilization?

4. 4 19-What would constitute a crossing over in an ascus? 20-Sketch one ascospore without crossing over, and another one with crossing over. 21-Count crossing overs in Experiment II. Were you accurate in your counting? 22-What does the frequency of crossing over allow scientists to do? 23- What is a map unit? How do you go about calculating the map distance between the color gene of the spores and the centromere in Sordaria? 24- Calculate the map distance using the formula given in this exercise. Show your work. 25- Why do you divide the percentage of crossing over by 2? 26-Compare Mitosis to Meiosis and check your answers. What is the one fact you learned in your answering the questions? 27- Take the self- quiz and write your answers below.