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20
DB                                 

                                   
       2008/08/07

     kaneko.satoko(at)ocha.ac.jp
molecular_function [GO:0003674]
   molecular transducer activity [GO:0060089]
       signal transducer activity [GO:0004871]
           receptor activity [GO:0004872]
                transmembrane receptor activity [GO:0004888]
                    G-protein coupled receptor activity [GO:0004930]
                        rhodopsin-like receptor activity [GO:0001584]
22.66kb               Reverse strand




8 exons, CDS 1446bp (481a.a.)
(5) processed (pseudo)gene,
                 unprocessed (pseudo)gene

                                        a
                             a'
                                                           unprocessed gene
                                                            duplicated gene
                  a
                 mRNA               splicing           

                                    
                                                            


                                        
                           a''
                 processed gene 
pseudogene   premature stop codon            (insertion)        (deletion)
ID   ID
1

        [apple mark+C]
  2 CotEditor
           [apple mark+V]
     makorin.fasta

               blat


    3       BLAST
1
    
                             [apple mark+V]




        2                       Mus musculus
    
    dna database

        3 Search Tool BLAT

       RUN

        BLAT
[apple mark+A]
          [apple mark+C]
CotEditor            [apple mark
+C] makorin.blat
[Alignment Locations vs. Karyotype]




[Alignment Locations vs. Query]
query


[Alignment Summary]
query
query
(6)                        
BLAST (Basic Local Alignment Search Tool)
                        alignment
                                                   

            
         
           
    
                     Arg Ser Val

        
             
       
                         Thr Ser Gly
                                                       Tyr    Val Arg
        
                 
       
                5'-tacgtcaggta-3'                
        
             
           
            
                                                   3'-atgcagtccat-5'
                                                       Met Gln Ser
        
                 
   
                
         Cys Ser Pro
                                                             Ala Val     His
        
             
       
            


BLAT (Blast Like Alignment Tool)
query/database 4,11(amino acid, DNA)-mer

     EST
080807
080807
080807
080807
080807
080807
080807
080807

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080807

  • 1. 20 DB 2008/08/07 kaneko.satoko(at)ocha.ac.jp
  • 2.
  • 3. molecular_function [GO:0003674] molecular transducer activity [GO:0060089] signal transducer activity [GO:0004871] receptor activity [GO:0004872] transmembrane receptor activity [GO:0004888] G-protein coupled receptor activity [GO:0004930] rhodopsin-like receptor activity [GO:0001584]
  • 4.
  • 5. 22.66kb Reverse strand 8 exons, CDS 1446bp (481a.a.)
  • 6.
  • 7.
  • 8. (5) processed (pseudo)gene, unprocessed (pseudo)gene a a' unprocessed gene duplicated gene a mRNA splicing a'' processed gene pseudogene premature stop codon (insertion) (deletion)
  • 9. ID ID
  • 10.
  • 11.
  • 12.
  • 13. 1 [apple mark+C] 2 CotEditor [apple mark+V] makorin.fasta blat 3 BLAST
  • 14. 1 [apple mark+V] 2 Mus musculus dna database 3 Search Tool BLAT RUN BLAT
  • 15.
  • 16. [apple mark+A] [apple mark+C] CotEditor [apple mark +C] makorin.blat
  • 17.
  • 18. [Alignment Locations vs. Karyotype] [Alignment Locations vs. Query] query [Alignment Summary] query
  • 19. query
  • 20.
  • 21.
  • 22.
  • 23.
  • 24. (6) BLAST (Basic Local Alignment Search Tool) alignment Arg Ser Val Thr Ser Gly Tyr Val Arg 5'-tacgtcaggta-3' 3'-atgcagtccat-5' Met Gln Ser Cys Ser Pro Ala Val His BLAT (Blast Like Alignment Tool) query/database 4,11(amino acid, DNA)-mer EST