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Types of Microscope
•‫کوپ‬‫س‬‫کرو‬‫ئ‬‫ا‬‫م‬‫ٹ‬‫س‬‫ا‬‫ر‬‫ی‬‫ن‬‫ک‬ ‫ز‬‫ی‬‫ف‬Phase Contrast Microscope
•‫کوپ‬‫س‬‫کرو‬‫ئ‬‫ا‬‫م‬‫ان‬‫ر‬‫کی‬‫ی‬‫ال‬Electron Micrscope
•‫کوپ‬‫س‬‫کرو‬‫ئ‬‫ا‬‫م‬‫ٹ‬‫سن‬ ‫لوری‬‫ف‬Flourescent microscope
Phase Contrast ‫ک‬‫س‬‫کرو‬‫ئ‬‫ا‬‫م‬‫ٹ‬‫س‬‫ا‬‫ر‬‫ی‬‫ن‬‫ک‬ ‫ز‬‫ی‬‫ف‬‫وپ‬
•‫ے‬‫ی‬‫ک‬ ‫ن‬‫ی‬‫ٹ‬‫س‬‫ر‬‫ی‬‫غ‬‫ی‬‫کو‬ ‫زوں‬‫ی‬‫چ‬‫ت‬‫ح‬‫ت‬‫کے‬ ‫ار‬‫ک‬ ‫ہ‬‫ق‬‫ی‬‫ر‬‫ط‬‫اس‬‫ے‬‫ہ‬‫ا‬‫ی‬‫ک‬‫س‬ ‫ا‬‫ج‬‫ا‬‫ی‬‫ک‬ ‫دہ‬‫اہ‬‫ش‬‫م‬ ‫ے‬‫وئ‬‫ہ‬
‫صوص‬‫خ‬‫م‬‫ھ‬‫ات‬‫س‬ ‫کے‬‫سر‬‫ن‬‫ی‬‫ڈ‬‫ی‬‫ک‬ ‫ں‬‫ی‬‫م‬ ‫ہ‬‫ق‬‫ی‬‫ر‬‫ط‬‫اس‬phase contrast‫د‬‫ا‬‫گ‬‫ل‬‫س‬‫ن‬‫ی‬‫ر‬‫آپ‬‫ا‬‫ئ‬
‫ے‬‫ہ‬‫ا‬‫ائ‬‫ج‬
‫کوپ‬‫س‬‫کرو‬‫ئ‬‫ا‬‫م‬‫ان‬‫ر‬‫کی‬‫ی‬‫ال‬Electron Micrscope
•‫ی‬‫ن‬‫ع‬‫ی‬‫ی‬‫ھوٹ‬‫چ‬ ‫ں‬‫ی‬‫م‬‫کوپ‬‫س‬‫کرو‬‫ئ‬‫ا‬‫م‬‫اس‬short wave length‫ی‬‫ن‬‫س‬‫رو‬‫کی‬
‫ے‬‫ہ‬‫ی‬‫وٹ‬‫ہ‬‫ال‬‫م‬‫ع‬‫ت‬‫س‬‫ا‬.‫ال‬‫ج‬‫م‬‫ی‬‫ب‬‫ان‬‫ر‬‫کی‬‫ی‬‫ال‬ ‫ور‬‫ت‬‫ق‬‫ا‬‫ط‬‫ک‬‫ئ‬‫ا‬vacum‫دی‬ ‫گزر‬ ‫سے‬‫ں‬‫ی‬‫م‬
‫کہ‬ ‫و‬‫ج‬‫ے‬‫ہ‬‫ی‬‫اٹ‬‫ج‬magnetic field‫ز‬‫ی‬‫چ‬‫س‬‫ج‬‫اور‬ ‫ے‬‫ہ‬‫ی‬‫اٹ‬‫ج‬‫ی‬‫وٹ‬‫ہ‬ ‫ں‬‫ی‬‫م‬‫دہ‬‫اہ‬‫ش‬‫م‬
‫ے‬‫ہ‬‫ی‬‫اٹ‬‫ج‬ ‫کی‬ ‫ی‬‫ش‬‫ک‬ ‫ر‬‫صوپ‬‫ی‬ ‫کی‬ ‫اس‬‫ے‬‫ہ‬‫ا‬‫وئ‬‫ہ‬ ‫ا‬‫ئ‬‫ر‬‫ک‬.
•‫کے‬‫م‬‫س‬‫ق‬ ‫اص‬‫ج‬‫ے‬‫ش‬‫ن‬‫س‬electromegnatic‫ں‬‫ی‬‫ہ‬‫ے‬‫وئ‬‫ہ‬.‫ی‬‫چ‬‫ھی‬‫ت‬‫شی‬‫ک‬‫کو‬ ‫ز‬
‫ے‬‫ہ‬‫ا‬‫ائ‬‫ج‬‫ا‬‫ھ‬‫ک‬‫ئ‬‫د‬‫کر‬ ‫ا‬‫ڑ‬‫پ‬‫اہ‬‫ی‬‫گ‬ ‫ار‬‫ز‬‫ہ‬‫اس‬‫چ‬‫ت‬‫سے‬‫ا‬‫ی‬‫گ‬ ‫ار‬‫ز‬‫ہ‬ ‫دس‬.‫ے‬‫ائ‬‫ج‬ ‫ھی‬‫ک‬‫ئ‬
‫د‬‫ک‬‫س‬ ‫ے‬‫ئ‬
‫و‬‫ہ‬ ‫ہ‬‫ن‬‫وں‬‫ت‬‫ک‬ ‫ی‬‫ہ‬‫کرون‬‫ی‬‫م‬ ‫دو‬‫ا‬‫ئ‬‫ک‬‫ئ‬‫ا‬ ‫ز‬‫ی‬‫چ‬‫والی‬.
FLOURESCENT MICROSCOPE
•‫ھ‬‫چ‬‫ک‬dye‫کی‬ultraviolet‫ے‬‫ہ‬‫ی‬‫وٹ‬‫ہ‬‫ت‬‫صن‬‫ا‬‫ج‬‫کی‬ ‫ے‬‫ی‬‫ک‬‫م‬‫چ‬ ‫ں‬‫ی‬‫م‬.
•Flourescent microscope‫ں‬‫ی‬‫م‬object‫کو‬
fluorochrome‫ے‬‫ہ‬‫ا‬‫ائ‬‫ج‬‫ا‬‫ی‬‫ک‬ ‫ن‬‫ی‬‫ٹ‬‫س‬‫سے‬.
•‫ج‬‫ا‬‫ڑ‬‫ھی‬‫چ‬‫کو‬ ‫ان‬ ‫ا‬‫ئ‬‫ے‬‫ہ‬‫ی‬‫اٹ‬‫ج‬ ‫دی‬‫ت‬‫ک‬‫ر‬‫ح‬ ‫ب‬‫چ‬‫کو‬ ‫ا‬‫ی‬‫س‬‫ا‬‫ض‬‫ع‬‫ی‬‫ے‬‫ہ‬‫ا‬‫ائ‬stimulate‫و‬‫ت‬
‫ں‬‫ی‬‫م‬‫شلہ‬‫ل‬‫س‬ ‫اس‬‫ے‬‫ہ‬‫ی‬‫ٹ‬‫ر‬‫ک‬ ‫ارج‬‫ج‬‫ی‬‫ن‬‫س‬‫رو‬‫وو‬ultraviolet‫ج‬‫لی‬ ‫مدد‬ ‫کی‬‫ئ‬‫او‬‫ع‬‫ش‬‫ی‬‫اٹ‬
‫کو‬ ‫ن‬‫ج‬‫ے‬‫ہ‬short wave blue rays‫ں‬‫ی‬‫ہ‬ ‫ے‬‫ی‬‫ہ‬‫ک‬.
Types of Fluorochrome
•Rhodamine
•Auramine
•‫اس‬ ‫ے‬‫ہ‬‫ا‬‫وئ‬‫ہ‬ ‫صود‬‫ق‬‫م‬‫ہ‬‫ن‬‫ب‬‫ا‬‫ع‬‫م‬‫ا‬‫ک‬ ‫ز‬‫ی‬‫چ‬‫س‬‫ج‬ ‫ں‬‫ی‬‫م‬‫صورت‬‫دوسری‬‫ے‬‫ی‬‫ک‬‫م‬‫چ‬ ‫صوص‬‫خ‬‫م‬‫کو‬
‫ی‬‫ن‬‫ب‬‫ا‬‫ارے‬‫س‬‫ت‬‫ہ‬‫ب‬ ‫ار‬‫ک‬ ‫ہ‬‫ق‬‫ی‬‫ر‬‫ط‬‫ہ‬‫ن‬‫ں‬‫ی‬‫ہ‬ ‫ے‬‫ی‬‫ل‬‫م‬‫ے‬‫وئ‬‫ہ‬‫ن‬‫ج‬،‫ی‬‫ا‬‫کو‬ ‫الپ‬‫م‬‫کے‬‫اڈی‬‫ئ‬‫ی‬‫ن‬‫ن‬
‫ے‬‫ہ‬‫ا‬‫وئ‬‫ہ‬‫ال‬‫م‬‫ع‬‫ت‬‫س‬‫ا‬ ‫ے‬‫ی‬‫ل‬ ‫کے‬‫ے‬‫ی‬‫ھ‬‫ک‬‫ئ‬‫د‬.
Lecture no. 7 microscope 3

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Lecture no. 7 microscope 3