2. Diesel engine oil is the major product of crude
It is a major source of pollution in our
Petroleum is a aromatic compound.
Oil is the Xenobiotic and recalcitrant.
Diesel oil spills on agricultural land generally
reduced plant growth.
Petroleum hydrocarbon can be degraded by
3. A large number of organism capable of
degrading polyaromatic hydrocarbons
Many different species , can degrade
petroleum compound such as pseudomonas,
micrococcus, bacillus, corneybactetium.
Hydrocarbon degrading bacteria are widely
distributed in marine fresh water and soil
Oil degrading organism basically used alkane
as a energy source and help in bioremediation.
4. Aims and objective
The Aim of our present
study to isolate the
petroleum engine oil
from garage soil.
The Aim present study
is to identify and
Bacteria are able to grow on to the enriched
media and used diesel engine oil as a energy
The bacteria have high potential to degrading oil
and producing biosurfactant layer due to which
reduce a surface tension of soil and water.
Isolated bacteria is Acinetobacter sp. may be.
Biochemical test were performed with
susceptibility was done with bacterial isolates.
It can be used to in big level of future purpose
using a gene &produce level for future.
12. FURTHER ASPECTS
Soil sample can stored in -80c for further
carry out PCR and sequencing.
identify 16s rna from potential bacteria.