GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Anti-GABAA Rδ antibody (STJ93192): http://www.stjohnslabs.com/gabaa-rd-antibody?filter_name=STJ93192 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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ANTIBODY CUSTOMERREVIEW
Findings: “Single band.” .- External Lab
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Protocol
Gel
Electrophoresis
12% SDS-PAGE. Membrane
wash
1X TBS-Tweenwash for 3 times.
Transfer
Transfer to nitrocellulose
Secondary
antibody
Probing
Secondaryantibody was added to
the solution of TBS 1:1000 dilution
ratio, for1 hr.
Blocking 1X TBST with 5% skimmed milk
powder for1 hr.
Membrane
Wash
1X PBST wash for 3 times
Primary
antibodyprobing
Primary antibody was added to
the solution of 1X TBST, 4˚C for
overnight
Visualisation ECL-PLUSimagining
Model Number STJ93192
Antibody Name Anti-GABAA Rδ antibody
Application Western Blot
Species Rat
Tissue Cerebellum
Dilution 1:1000
Secondary Antibody goat anti-rabbit HRP
Dilution: 1:5000
Fig 1. Western blot after immunoprecipitation
(antibody used for both applications).The antibody did not
very well immunoprecipitate the antigen, but showed a
single band at the right molecular weight in the Western
blot. 1 = input, 2 = flow-through, 3 = eluate (sample buffer
withoutreducingagent, so upper band very likely due to
co-eluted antibody).