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Inclusion bodies
1. Viruses cause…
Cell death (cytocidal effect)
Eg: Poliovirus.
Cellular proliferation
Eg:Molluscum contagiosum.
Malignant transformation
Eg:Oncogenic viruses.
Some virus and host cell enter into a peaceful
coexistence-’Steady state infection’.
2. Seen in tissue culture
Viral infection of cells leads to readily observable
cellular changes called cytopathic effects
Enteroviruses- crenation of cells and degeneration
of cell sheet HEMA
Measles- syncytium formation
Adenovirus – bunch of grapes
Herpes virus – discrete focal degeneration
3. Cytopathic effects
Lead to observable cellularLead to observable cellular
changes in tissue culture.changes in tissue culture.
4. Inclusion bodies….(SN,MCQs)
Structures with distinct size, shape, location and
staining properties that can be demonstrated in virus
infected cells under the light microscope.
CLASSIFICATION
They may be situated at:
Cytoplasm (intracytoplasmic)- pox virus
Nucleus (intranuclear)- herpes
Both - Measles
5. Appearance of Inclusion body….
• They are generally acidophilic.
• Seen as pink structures when stained with giemsa or
eosin methylene blue.
• Some viruses form basophilic inclusions.
Eg: Adenovirus.
6. General features…
May be crystalline aggregates of
virions.
Made of virus antigens present at the
site of virus synthesis.
Represent degenerative changes
produced by viral infection.
7. Intracytoplasmic inclusion bodies
Negri bodies-Presumptive diagnosis of rabies.
Guarineri bodies- small multiple inclusions.
eg- Vaccinia(small pox) virus.
PASCHEN Bodies
Bollinger bodies- large inclusions.
eg- Fowl pox virus.
Molluscum bodies- very large 20-30µ.
eg- Molluscum contagiosum.
15. Intranuclear inclusion bodies
Classified by Cowdry- 2types
Cowdry type A inclusions: variable and
granular
Eg-herpesvirus, yellow fever virus.
Cowdry type B inclusions: circumscribed and
multiple
Eg- Adenovirus, Poliovirus.