1. F505 constitutively active
A273 inactive mutant
1. James, PW (2000) Semin. Immunol. 12:23-34.
Kabouridis, PS (1997) EMBO. J 16: 4983-4998.
Yurchak, LK. (1995) Mol. Cell. Biol. 15, 6914-6922.
2. Kosugi, A. (2001) Immunol. Lett. 76:133-138.
Yurchak, LK. (1995) Mol. Cell. Biol. 15, 6914-6922.
Rodgers, W. (1994) Mol. Cell. Biol. 14, 5384-5391
SH4 –Lipid Modification
Unique
File: Lck 1
SH3 -Proline-Rich Domain Binding
SH2 -Phosphotyrosine Binding
SH1 - Catalytic Domain
505
394
20
22
-Cysteine
-Tyrosine
-Lysine
273
14
88
250
516
Gly2-Cys3-X-X-Cys5
Myr Palm Palm 1
Lipid Microdomains 2
SH3
SH2
p56lck
M:CH3
(CH2
)12
COO-
P:CH3
(CH2
)14
COO-