1. Neonatal Jaundice
Dr. Kalpana Malla
MD Pediatrics
Manipal Teaching Hospital
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3. Normal Physiology
โข Bilirubin -breakdown of hemoglobin
โข Unconjugated bilirubin (insoluble in water)
transported to liver- Bound to albumin
โข Transported into hepatocyte (Ligandin / y-
protein ) & conjugated - With glucuronic acid
โ now water soluble
โข Secreted into bile
4. Normal Physiology
โข Secreted into bile
โข In ileum & colon, converted to stercobilin
โข 10-20% (Deconjugated by ฮฒ glucuronidase)
reabsorbed into portal circulation
(Enterohepatic circulation )and re-excreted
into bile or into urine by kidneys -
urobilinogen
6. NEWBORN JAUNDICE
(PHYSIOLOGICAL)
Etiology
1. Decreased RBC survival 90 days, increased RBC
vol /Kg, polycythemia of NB
2. Poor hepatic uptake due to immature liver-
decreased ligandin or Y- protein
3. Poor conjugation due to enzyme deficiency-
UDPG-T activity
7. NEWBORN JAUNDICE
(PHYSIOLOGICAL)
4. Increased enterohepatic circulation due to
- High level of intst beta-glucoronidase
- delayed colonization by bacteria
- Decreased gut motility
5.Decreased hepatic excretion of bilirubin
8. PHYSIOLOGICAL JAUNDICE
โข Seen both in term and preterms
โข Self limiting
โข Develops after 24 hours
โข Peaks by day 4- 5 in terms and day 7-8 in
preterms
โข Peak levels -12mg/dl in term & 15mg/dl in
preterm
โข Gradually subsides by 10-14 days
โข No Treatment necessary
9. PATHOLOGICAL JAUNDICE
Suspect if...โข Jaundice in first 24 hours
โข Rise of >5mg/24 hours or 0.5
mg/dl/hr
โข Jaundice beyond physiological
limits
โข Conjugated bilirubin- >2mg or 20%
of total
โข Beyond 2 weeks
โข Signs of underlying illness ++
10. Pathological Jaundice - Hemolytic
causes (unconjugated)
Coombs' test positive Coombs' test
โ Rh incompatibility negative
โ ABO โ Red blood cell
incompatibility membrane defects
โ Red blood cell
enzyme defects
โ Drugs
โ Hemoglobinopathies
โ Sepsis
18. After 72 hrs (within 2 weeks)
โข Septicemia
โข Neonatal Hepatitis, other IU infections
โข Extra hepatic Biliary atresia
โข Breast milk jaundice
โข Metabolic diseasesโgalactosaemia, CF, alpha-
1 antitrypsin deficiency, hypothyroidism
โข Hypertrophic Pyloric stenosis
19.
20.
21. Diagnosis
1)HistoryโAntenatal
Drugs
Trauma
Family H/O of jaundice
Liver disease
H/O delayed feeding
Sepsis
Sibling jaundice
Splenectomy in family
22. 2. General exam
โข Cramerโs Index
1.Face-4-6 mg/dl
2.Chest &Upper trunk โ 8-10 mg/dl
3.Lower abdomen,thigh-12 -14mg/dl
4.Forearms &lower legs -15 -18 mg/dl
โข Palms & sloes->15-20 mg/dl
24. 3) Lab investigations
1. Hemoglobin, PCV with peripheral smear
2. Total Bilirubin (Total / Direct & Indirect)
- >12 mg /<24hr
- <12 mg/ >24 hr
3. Bilirubin level โSpecial tests โ
โ TORCH titres - Thyroid function tests
โ Metabolic work up - Sepsis screen
โ USG / X ray abdomen
โข Blood group and Rh typing
โข Reticulocyte count
25. Investigations in RH incompatibility
โข Antenatal - (mother Rh-ve, previous baby Rh
+ ve, father Rh +ve.
1) H/o of abortion, H/o having taken Anti D
gammaglobulin
2) USG for baby maturation ,HSM, ascites,
hydrominos, gen. anasraca
26. Investigations in RH incompatibility
โข Antenatal -
- Blood grp (ABO & Rh) of father ,earlier baby
- Indirect Coombโs test โ to detect antibodies in
motherโs serum
IgG Anti body Titre to D TO be estimated at 12-16,28-
32 and 36 weeks. If anti D antibody Titre 1:16 it
should be tested serially
- Ab titre in motherโs blood ->1:64 dignostic of HDN-
TO CONSIDER TERMINATION OF PREGNANCY.
27. Investigations in RH incompatibility
โข Anmiocentesis:
- Look for lecithin sphingomyelin ratio to suggest
maturity.
- Shake test for 15 sec. with equal vol etanol 95%-
allowed to stand-ring of buble at the disc
- Optical density-by spectrophotometer OD.>0.15
denotes maturity of lungs
- Alpha feto protein level increased โrh issoimun
- Fetal bloob grp prenatally โ amniocentesis
28. POSTNATAL INVESTIGATION BABY
Cord bloodโall babies of Rh-ve mothers, all Unknown
blood groups, all with prior h/o jaundice in earlier
babies
Blood group-both mother and baby
- For evidence of hemolysis โ
Direct Coombs test
Reticulocyte count - >10 suggest hemolysis.
Hemoglobin cord
Peripheral smear -RBC morphology
Bilirubin
39. Phototherapy
โข Safe and effective method for treatment of
neonatal jaundice
โข Bilirubin absorbs light maximum at 420-460
nm
40. Mechanism of Action
Conversion of insoluble Bilirubin into soluble
bilirubin
1.Photo-isomerization-conversion into soluble
form โ takes place in extravascular space of skin โ
conversion to less toxic polar isomer-diffuses into the
blood โexcreted easily into bile
2.Structural isomerization - conv to lumirubin -
rapidly excreted in bile and urine
3. Photo-oxidation- of Bilirubin to water soluble
polymers colourless by product.
41. Indications for Phototherapy
โข TSB > 15 mg % in term
โข TSB > 12 mg% in preterm
โข TSB > 5 mg% within 24 hours
โข Adjuvant to exchange transfusion
โข Prophylactic PT โ ELBW, bruised babies,
hemolytic disease of NB,VLBW with
Perinatal risk factors
42. Indications
โข Precautions
โ Cover the eyes and Genitals
โ Supplemental hydration
โ Watch for side effects
43.
44. Procedure
โข Best is narrow spectral blue lights (425-
475nm)
โข White lamps (380-700nm)
โข Distance from skin โ 45cm
โข Intensive PT โ 15-20 cm
โข Shield eyes & genitalia
โข Space of 5-8cm between phototherapy
unit & incubator
45. โข Double surface PT โ can be given by
fiber-optic blankets (biliblankets)
โข Change position once in every 2-4 hrs
โข Skin bleached by PT
โข Level to be checked every 10-20 hrs
โข Frequent temperature monitoring &
daily weight check
47. โข Late โ
โ Risk of skin malignancies
โ Damage to intracellular
DNA
โ Retinal damage
โ Disturbance in circadian
rhythm
Testicular damage
49. DRUGS
โข Phenobarbitone โ increase y and z ligands
-induces liver ezymes - โโ conjugation๏
phenobarbitone
โข Metalloporphyrins (tin and zinc
porphyrins and meso prophyrins)
-inhibits heme oxygenase
50. โข IVIG - Inhibit haemolysis
โข Oral agar, Cholestyramine-โ enterohepatic
circ
โข Albumin infusions๏ Inc albumin binding
51. โข Exchange blood transfusion -- changing
the babies blood with the other blood.
โข Usually in hemolytic disease of
newborn.
โข It removes partially hemolysed and
antibody coated RBCs and also
billirubin
52. Methods of exchange
โข Single volume exchange- 80ml/kg
โข Double volume exchange- 160ml/kg
(87% of infant blood volume exchanged
with new blood)
โข Triple volume exchange.
54. Exchange Transfusion
Indications:
โข Rh and ABO incompatibility
โข Unconjugated billirubin > 20 -25mg/dl in
term, >15 -18mg/dl preterm babies. Sick
neonates exchange at lower level
โข Septicemia /DIC/ sclerema
โข Neonatal ITP
โข Severe anemia due to any cause with HF
55. Exchange Transfusion
(Indications)
โข Early Kernicterus
โข Cong H Sperocytosis
โข G-6- PD deficiency
โข Hepatic coma
56. In Hemolytic disease of the
newborn (ABO / Rh)
โข H/O previous severely affected infant
โข Cord Hb <10gm% & bilirubin > 5mg/dl
โข Rate of rise of bilirubun > 0.5mg/100ml/hr
โข Jaundice in first 24 hrs of life
โข Signs of hemolysis-clinical or lab
โข Maternal ab titer > 1in 64
โข Positive DCT
โข Preterm LBW with hyperbilirubinemia
โข Reticulocyte >10
57. Rh incompatibility
โข Due to Rh D-Ag
โข < 1 mL of Rh-positive fetal blood is sufficient to
sensitize the mother
โข 90% sensitization during delivery/abortion
โข So , most first born infants are not affected due
to the short period of exposure which is
insufficient to produce a significant maternal Ig G
antibody response.
58. Rh incompatibility
โข Sensitized mother produces Ab โIgG typesโ
crosses placenta
โข Once sensitized โsmall doses of Ag stimulate
high Ab titer .
โข So, risk and severity of sensitization response
increases with each subsequent pregnancy with
Rh-positive blood fetus
59. ABO incompatibility
โข Mother is type O and the baby is either type A or
B.
โข O +ve Mothers makes antibodies which are IgM
& (IgG) types - IgG types crosses the placenta
โข No effects if the mother & baby have same blood
group or baby is grp O, as there is nothing to
make antibodies against.
60. ABO incompatibility
โข If mother - type A or B Makes antibodies
(IgM) type so does not cross the placenta
So, even if baby has a different blood type no
effect
61. Selection of blood
โข Blood group O โ no antigen
Ab โanti -A, anti-B
โข Blood group A โ antigen A
Ab - Anti-B
โข Blood group B โantigen B
Ab โ anti -A
63. Selection of blood
โข In Rh incompatibility: (O,A,B,AB-Negative)
choice -Rh negative โ
- Preferably babyโs ABO
- O group cross matched against maternal
serum
โข In ABO incompatibility โ โOโ blood group same as
babyโs Rh ( +/-) with low titre of Anti A and Anti B
antibodies OR ABO type specific blood cross
matched against infant serum
- Septicemia โ Same as babyโs ABO and Rh
65. Procedure
โข IN NICU OR OT
โข Radiant warmer, Monitor HR, BP and other
vitals, infants arms and legs are restrained.
โข Assistant to record volume in & out, to
check vitals.
โข Blood pre warmed to 37 c
โข Dried umbilical cord soaked with wet
gauze.
โข Canulation of umbilical vein- 12 oโclock
66. โข Catheter inserted till free flow of blood
or SHOULDER UMBILICAL LENGTH.
โข Small aliquots of blood removed 5
to10ml -PUSH PULL method.
โข Blood in the bag gently mixed.
โข Procedure over 1 to 2 hr.
โข Tie around the cord for 1 hr, or hold
tightly at the end of procedure.
67. Complications
โข Hypocalcemia and Hypomagnesemia -
Citrate in CPD blood.
โข Hypoglycemia
โข Metabolic alkalosis or acidosis.
โข Hyperkelemia.
โข CVS: overload and arrythmias
โข Infections: HBV HIV
โข Hemolysis
โข Hypothermia, NEC.
68. Other roots for exchange
โข Umbilical vein cut down- incision
above umbilicus in midline.
โข Femoral vein canulation with radial
artery canulation.
70. Breast milk jaundice
โข Late onset
โข Due to factors in breast milk โInterfere with
bilirubin conjugation:
- Pregnanediol
- Free fatty acids
- ฮฒ-glucoronidase
โข Instead of โby 7 days it continues to rise may
go upto 20-30mg/dl by 2nd-3rd wks of age &
return to normal by 4-12 wks
71. Management
โข Stop breast feeding -48 hrs
โข Again resume it, bilirubin may rise again but
not reach previous high level
72. Breast feeding jaundice
โข Decreased intake of milk leads to increased
enterohepatic circulation
โข Higher levels on day 4 compared to
formula fed babies due decreased
intake of milk
73. Prevention
1. Anti D to be given to the mother after delivery of
the baby-within 48hrs. Also can be given to all
unsensitized mothers at 28-32 weeks of
gestation
2. Amniocentesis and IU transfusion to severely
affected babies
3. Preterm delivery of severely affected babies
4. Cord blood studies-followed by Phototherapy
5. Exchange transfusion
74. KERNICTERUS
โข Entry of unbound bilirubin into brain as
free or albumin bound bilirubin
โข Acidosis affects bilirubin solubility
โข Hyperosmolarity, anoxia and hypercarbia
disrupt BBB
75. โข Yellow staining of brain assc with
neuronal injury
โข Affects basal ganglia, cranial nerve
nuclei, brain stem nuclei, hippocampus
and AHC of spinal cord (cortex usually
spared)
โข Necrosis, neuronal loss and gliosis
โฆpathological findings
76. ACUTE BILIRUBIN
ENCEPHALOPATHY
โข STAGE 1: hypotonia, lethargy, high
pitched cry and poor suck (D1-3)
โข STAGE 2: hypertonia, opisthotonus,
rigidity, oculogyric crisis, retrocollis,
fever, seizures. (2nd week)
โข Those who survive develop chronic
bilirubin encephalopathy
โข STAGE 3: Hypotonia replaces hypertonia
after 3rd week age
77. CHRONIC BILIRUBIN
ENCEPHALOPATHY
โข Choreo-Athetosis
โข Partial or complete sensorineural hearing
loss
โข Limitation of upward gaze
โข Dental dysplasia
โข Intellectual deficits
78. LOW BILIRUBIN KERNICTERUS
โข In LBW babies, preterms
โข Overt changes not seen
โข Other factors: IVH, drugs, benzyl alcohol
โข More likely to suffer from anoxia,
hypercarbia and sepsis
79. TREATMENT
โข Phototherapy
โข Exchange transfusion
โข Albumin infusion
โข Anticonvulsants: phenobarbitone
โข BERA at follow up