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Mamatha Chivukula MD, FASCP, FCAP Assistant Professor  Associate Director of Immunohistochemistry lab  Magee-Women’s Hospital of UPMC Pittsburgh, PA Study cases and application of  two-color immunochemistry
Objectives   ,[object Object],[object Object]
Cytomorphology of ASC-H ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Case 1 ,[object Object]
 
 
Groups of atypical cells  (without distinct cell borders)
-
Hyperchromatic nuclei  - Coarse to fine chromatin
[object Object]
 
“ Syncytial cell” pattern Source:  Chivukula M ,  Shidham V . CytoJournal 2006, 3:14
Case 2 ,[object Object]
[object Object],[object Object]
[object Object]
 
p16
KI -67
Atrophic “ Single-cell” pattern Source:  Chivukula M ,  Shidham V . CytoJournal 2006, 3:14
Case 3 ,[object Object]
 
Cohesive clusters
-Prominent nucleoli - Low N/C ratio
[object Object]
 
“ School of fish” Repair – like pattern Source:  Chivukula M ,  Shidham V . CytoJournal 2006, 3:14
Case 4 ,[object Object]
 
 
 
[object Object]
 
“ Isolated cell pattern” Source:  Chivukula  M ,  Shidham  V . CytoJournal 2006, 3:14
Case 5 ,[object Object]
 
- HCG of parabasal cells - Small dark nuclei - Variable, usually scant cytoplasm
[object Object]
 
Atrophic “ Parabasal cell groups” Source:  Chivukula M ,  Shidham V . CytoJournal 2006, 3:14
Application of two-color immunochemistry ,[object Object],[object Object],[object Object]
[object Object],[object Object],State of the art….work in progress
 
 
[object Object],Thank you

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02 Asc H Continued Chivkula